Urine oxalic acid content detection system and detection method

A detection method, the technology of oxalic acid, which is applied in the direction of measuring devices, preparation of test samples, instruments, etc., can solve the problems of high requirements for operators, interference of inorganic acids, expensive instruments, etc., and achieve accurate detection results, stable detection, and easy operation. convenient effect

Pending Publication Date: 2021-11-16
CHONGQING BIOSTEC BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the interference of inorganic acids in the separation process of high-pressure liquid chromatography is serious, and the equipment...

Method used

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  • Urine oxalic acid content detection system and detection method
  • Urine oxalic acid content detection system and detection method
  • Urine oxalic acid content detection system and detection method

Examples

Experimental program
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Embodiment 1

[0051] Urine oxalate content detection system, including pretreatment kit and detection kit;

[0052] The pretreatment kit includes:

[0053] The pretreatment kit includes:

[0054] Sample processing tube and sample processing liquid, the sample processing liquid includes the following components:

[0055]pH5.7 buffer, EDTA and proclin300; the concentration of the pH5.7 buffer in the sample treatment solution is 0.1M, the concentration of EDTA is 2Mm, and the mass fraction of proclin300 is 0.2%;

[0056] The weight of activated carbon in the sample processing tube is 0.1g;

[0057] The detection kit includes R1 reagent and R2 reagent;

[0058] The R1 reagent comprises the following components:

[0059] Citric acid, DMAB and MBTH, the concentration of the citric acid in the R1 reagent is 0.07M, the concentration of the DMAB in the R1 reagent is 0.18mM; the concentration of the MBTH in the R1 reagent is 0.09mM;

[0060] R2 reagents include the following components:

[0061...

Embodiment 2

[0069] This embodiment is based on embodiment 1, and the difference with embodiment 1 is that the concentration of each component is different, specifically:

[0070] The concentration of the pH7.8 buffer solution in the sample treatment solution is 0.1M, the concentration of EDTA is 9Mm, and the mass fraction of proclin300 is 0.02%;

[0071] The weight of activated carbon in the sample processing tube is 0.07g;

[0072] The detection kit includes R1 reagent and R2 reagent;

[0073] The R1 reagent comprises the following components:

[0074] Citric acid, DMAB and MBTH, the concentration of the citric acid in the R1 reagent is 0.2M, the concentration of the DMAB in the R1 reagent is 0.29mM; the concentration of the MBTH in the R1 reagent is 0.2mM;

[0075] R2 reagents include the following components:

[0076] The buffer solution, oxalate oxidase and peroxidase that pH value is 5.8; The concentration of described buffer solution in R2 reagent is 0.2M, and the concentration o...

Embodiment 3

[0078] This embodiment is based on embodiment 1, and the difference with embodiment 1 is that the concentration of each component is different, specifically:

[0079] The concentration of the pH6.5 buffer solution in the sample treatment solution is 0.1M, the concentration of EDTA is 3Mm, and the mass fraction of proclin300 is 0.1%;

[0080] The weight of activated carbon is 0.5g;

[0081] The detection kit includes R1 reagent and R2 reagent;

[0082] The R1 reagent comprises the following components:

[0083] Citric acid, DMAB and MBTH, the concentration of the citric acid in the R1 reagent is 0.15M, the concentration of the DMAB in the R1 reagent is 0.2mM; the concentration of the MBTH in the R1 reagent is 0.15mM;

[0084] R2 reagents include the following components:

[0085] The buffer solution, oxalate oxidase and peroxidase that pH value is 5; The concentration of described buffer solution in R2 reagent is 0.13M, and the concentration of described oxalate oxidase in R...

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Abstract

The invention discloses a urine oxalic acid content detection system and detection method. The detection system comprises a pretreatment kit and a detection kit; the pretreatment kit comprises activated carbon and a sample treatment solution, wherein the detection kit comprises a reagent R1 and a reagent R2; the R1 reagent is prepared from the following components: citric acid, DMAB and MBTH; the reagent R2 comprises the following components: a buffer solution with the pH value of 5.6, oxalate oxidase and peroxidase; the concentration of the buffer solution in the R2 reagent is 0.1 M, the concentration of the oxalate oxidase in the R2 reagent is greater than 50 U/L, and the concentration of the peroxidase in the R2 reagent is greater than 5000 U/L; and the R2 reagent is in a dry powder state and is redissolved by water when being used. The method has a high detection limit, can be used for detecting the content of oxalic acid in urine, has high detection sensitivity, and can eliminate interference of substances such as vitamin C in urine, so that the detection result is more accurate.

Description

technical field [0001] The invention relates to the technical field of oxalic acid content detection in urine, in particular to a urine oxalic acid content detection system and a detection method. Background technique [0002] Oxalic acid has a strong chelation effect on calcium, so it can affect the body's absorption and metabolism of calcium. First, it can form a stable non-water-soluble chelate with dietary calcium, making it difficult for calcium to enter the environment in the human body and be absorbed by the body. Secondly, the oxalic acid produced by the human body through food intake and metabolism can form precipitation with calcium in the body and accumulate in the glomeruli, which is the main cause of urinary stones. The concentration of oxalic acid in urine is closely related to urinary stones. Accurate detection of urinary oxalate content is of great significance for basic research and clinical treatment of urinary stones. The determination methods of oxalic ...

Claims

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Application Information

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IPC IPC(8): G01N33/493G01N1/38G01N1/34G01N35/00
CPCG01N33/493G01N1/38G01N1/34G01N35/00
Inventor 黄性义
Owner CHONGQING BIOSTEC BIOTECH
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