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Application of PDCD4 regroup expression vector on process for preparing medicine curing glioma

An expression vector and glioma technology, applied in application, drug combination, anti-tumor drugs, etc., can solve the problems of solid tumors such as poor effect, easy drug resistance, and high cytotoxicity, so as to improve prognosis and have good application prospects , the effect of improving the quality of life

Inactive Publication Date: 2008-06-25
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of traditional anticancer drugs include: relatively narrow therapeutic window, poor effect on solid tumors, large adverse reactions, and easy drug resistance after long-term use
However, the first and second-generation adenoviral vectors still have many shortcomings, such as small packaging capacity, high cytotoxicity, strong immunogenicity and short expression time, etc.

Method used

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  • Application of PDCD4 regroup expression vector on process for preparing medicine curing glioma
  • Application of PDCD4 regroup expression vector on process for preparing medicine curing glioma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0041] The recombinant expression vector preferably used in the test of the present invention is a viral vector (purchased from Wuhan University Institute of Virology), and preferably used in the viral vector is an adenovirus:

[0042] 1. Construction and Production of PDCD4 Recombinant Adenovirus

[0043] (1) Construction of adenovirus expression vector

[0044] 1) Cloning of PDCD4 gene into pMD-18T plasmid: Design a pair of specific oligonucleotide primers according to the cDNA sequence of PDCD4 (including signal peptide). The upstream primer sequence is: 5'-CTGAGCACATATGATGAATCAAACTGCGATTCTGAT-3', and the downstream primer sequence is: 5'-GAGGATCCTTAAGGAGATCTTTTTAGACATTTC-3'. Using the phage human lung cDNA library as a template, using the above-mentioned upstream and downstream primers, the target gene PDCD4 was amplified with Pyrobest high-fidelity DNA polymerase. The PCR parameters are 94°C, 30s; 53°C, 1min; 72°C, 1min, 30 cycles. The PCR products were recovered by 1%...

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Abstract

This invention discloses the application of a recombinant expression vector PDCD4 in preparation of glioma pharmaceuticals and in treatment of glioma. The invention prepares the glioma chemical pharmaceuticals and treats the glioma by inserting nucleotide sequence with gene of PDCD4 code into the recombinant expression vector, facilitating highly effective expression of the gene of PDCD4 code in tumour cell to treat malignant brain tumor and restrain the growth and malignancy thereof. The invention provides powerful experiment basis for development of anti-cancer pharmaceuticals and possesses important theoretical value and promising potential application.

Description

technical field [0001] The invention relates to the application of a PDCD4 recombinant expression vector, in particular to the application of a PDCD4 recombinant expression vector in the preparation of drugs for treating glioma. Background technique [0002] (1) Promoting tumor cell apoptosis is one of the development directions of current anticancer drug research [0003] Currently, cancer ranks second in the cause of death worldwide, second only to heart disease. Taking the United States as an example, 500,000 people died of cancer in 2000. Traditional anticancer drugs are mostly cytotoxic drugs, and their clinical efficacy often depends on the sensitivity of cancer cells to drugs. The main disadvantages of traditional anticancer drugs include: relatively narrow therapeutic window, poor effect on solid tumors, large adverse reactions, and easy drug resistance after long-term use. [0004] Studies in recent years have found that the expression of certain genes involved i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00C12N15/861A61P35/00
Inventor 张利宁高飞王晓燕张霞王群朱法良郭春
Owner SHANDONG UNIV
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