Competitive particle immunoassay methods utilizing fluorescence microscopy

A fluorescent microscope, microparticle technology, applied in the field of competitive particle immunoassays using fluorescence microscopy, which can solve the problem of unreliable determination of sex and other characteristics of unhatched birds

Inactive Publication Date: 2008-07-09
EMBREX INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although conventional candling techniques are relatively effective at differentiating between live and dead eg...

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  • Competitive particle immunoassay methods utilizing fluorescence microscopy
  • Competitive particle immunoassay methods utilizing fluorescence microscopy
  • Competitive particle immunoassay methods utilizing fluorescence microscopy

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Embodiment Construction

[0026] The invention will now be explained more fully hereinafter with reference to the accompanying drawings, in which preferred embodiments of the invention are shown. This invention may, however, be embodied in different forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that those skilled in the art will fully convey the scope of the invention fully. Like numbers refer to like elements throughout. In the drawings, the thickness of some lines, layers, components, elements or features may be exaggerated for clarity. Dashed lines illustrate optional features or operations unless indicated otherwise.

[0027] The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the singular forms "a", "an", and "the" are intended to include the plural unless the context clearly indicates otherwise. It should be furt...

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Abstract

The present invention provides a method for measuring an analyte in a sample by a competitive immunoassay using fluorescence microscopy. In a specific embodiment, the present invention provides a method for determining the sex of an avian embryo in ovo by determining the presence of an estrogenic steroid compound in a sample of embryonic fluid (eg, allantoic fluid or blood) of an avian egg (Figure 1).

Description

[0001] Related application materials [0002] This application claims the benefit of US Patent Provisional Application No. 60 / 679,717, filed May 11, 2005, the contents of which are hereby incorporated by reference in their entirety. field of invention [0003] The present invention relates generally to methods of measuring analytes in a sample, and more particularly to methods of measuring analytes using competitive particle immunoassays. Background of the invention [0004] Egg discrimination based on certain observable qualities is a well known and long practiced practice in the poultry industry. Candling is a common term for one such technique, a term derived from the custom of examining eggs by candlelight. As those familiar with eggs know, although the shell may appear opaque under the best lighting conditions, it is actually somewhat translucent, allowing the egg's contents to be seen when held in front of direct light. [0005] In hatchery management, it may be des...

Claims

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Application Information

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IPC IPC(8): G01N33/00G01N33/53G01N33/543G01N21/76
CPCA01K45/00A01K45/007G01N21/6452G01N33/5005G01N33/54313G01N33/582G01N33/743G02B21/16
Inventor J·特茨科夫斯基D·马哈托A·查尔克
Owner EMBREX INC
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