Genus Miscanthus Miscanthus giganteus tissue culture propagations method

A technology of Qigang group and plant, which is applied in the field of Qigang tissue culture, rapid propagation and complete production technology to achieve the effects of robust root system, high proliferation rate and stable proliferation.

Inactive Publication Date: 2008-08-27
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Qigang tissue culture rapid propagation techn

Method used

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  • Genus Miscanthus Miscanthus giganteus tissue culture propagations method

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Embodiment 1

[0022] In late June 2005, the 6-leaf-year-old Qigang stems were cut in the Xiaotangshan base of the Beijing Grass Industry and Environmental Research and Development Center, and the 4-5 stem nodes upward from the base were cut, leaving 1.5cm at the top and bottom, and the leaves were removed. Keep the leaf sheath, first spray the surface with 70% alcohol, then soak it with 1% sodium hypochlorite solution in the ultra-clean workbench for 20 minutes, and then rinse it with sterile water for 3 times. Cut off 0.5 cm from the upper and lower stem nodes and inoculate them on the axillary bud induction medium. After 10 days, when the axillary buds grow to a height of 2-3 cm, cut the base of the axillary buds as explants and inoculate them on the primary culture medium for cluster bud induction, at 26±1°C, light for 16 hours a day, and the light intensity is 40-50 μmol m -2 the s -1 Clustered shoots were obtained after 30 days of induction under the same conditions, and the induction...

Embodiment 2

[0028] The culture procedures were all carried out according to Example 1, the difference was that the cutting time of the odd-gang stem node was the 8-leaf stage in mid-July, the primary culture explant was the middle segment of the axillary bud, and the cluster bud induction medium was MS medium Additional 6-benzylaminoadenine 6-BA 4mg / L, NAA 0.1-0.5mg / L, 6 H 2 O Magnesium Chloride MgCl 2 ·6H 2 O 500-1000mg / L, sucrose 20g / L, crystal agar Gelrite 2g / L. The subculture medium is MS medium supplemented with 6-benzylaminoadenine 6-BA 3mg / L, NAA 0.3mg / L, 6 H 2 O Magnesium Chloride MgCl 2 ·6H 2 O500mg / L, sucrose 20g / L, crystal agarose Gelrite 2g / L. The rooting induction medium for clustered shoots is: 1 / 2MS medium, NAA 1.5mg / L, magnesium chloride Mgcl 2 500mg / L, sucrose 20g / L, crystal agarose gelrite 3g / L, activated carbon 5g / L. The multiplication coefficients were all 5, and the rooting rate was 72%.

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Abstract

The invention provides a vitro propagation technology of miscanthus anderss giganteus. The technology includes a process of obtaining giganteus regenerated plantlet by induction from the primary culture of buds to the secondary reproduction culture, the root induction of the axillary buds and the acclimation transplant with the giganteus sterile with the sterile giganteus axillary bud as the explants. The technology has a high axillary bud growth coefficient, an orderly rootage, and a high survival rate after the vitro propagation of the acclimation transplant, which is applicable to the commercial production of the giganteus plant seeds.

Description

technical field [0001] The invention relates to the tissue culture rapid propagation technology of Miscanthus Xgiganteus. Specifically, it involves the complete production technology of using Qigang sterile axillary buds as explants, obtaining clustered buds through organogenesis, and multiplying, rooting, domesticating and transplanting in large quantities. Belongs to the field of biotechnology. Background technique [0002] Qigang is a perennial herbaceous plant of Gramineae, which is a natural hybrid triploid whose parents are M. ). In the natural environment, Qigang reproduces by rhizomes. New plants grow from the rhizomes from April to May every year. The height of the plants is 3-4 meters from August to September. Inflorescences are drawn out in October, and the aboveground parts wither in December. The leaves are slender and sword-shaped throughout the growth period, and the color is bright green; the stem is node-shaped, hollow, and has 12-13 nodes when mature; th...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00C12N5/04
Inventor 武菊英陈慧萍段留生滕文军
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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