Cell migration assay

A cell migration, cell technology, applied in the field of preparation and use of these compositions, compositions for transendothelial migration assays, can solve problems such as being unsuitable for high-throughput screening, complex preparation, etc.
CN101263223AInactive Publication Date: 2008-09-10GE HEALTHCARE BIO SCI CORP
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
GE HEALTHCARE BIO SCI CORP
Publication Date
2008-09-10
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The present invention provides compositions and methods for preparation of a three-dimensional transendothelial cell migration (TEM) assay. These compositions and methods are uniquely suited for the high throughput TEM assay, and for the analysis and identification of TEM mediators which inhibit or stimulate this process. The composition for detecting migration of cells comprises a solid layer comprising collagen gel; a first cellular layer in contact with the solid layer and comprising a first cell type; and a second cell type seeded on top of the first cellular layer. Optionally, gelatin is included in the collagen gel. A 96 well plate format is disclosed, the combination with a high throughput cellular scanner enables high throughput TEM assay.
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Description

[0001] Cross References to Related Applications

[0002] This application claims priority to U.S. Provisional Patent Application No. 60 / 718,057, filed September 16, 2005, and U.S. Provisional Patent Application No. 60 / 747,430, filed May 17, 2006, which disclose The contents of all are incorporated herein by reference. field of invention

[0003] The present invention generally relates to methods for assaying hematodialysis. More particularly, the present invention relates to compositions for use in transendothelial migration assays, and methods of making and using these compositions. Background of the invention

[0004] Migration of cells across the vascular endothelium is an important event in the pathophysiology of conditions like inflammation, arteriosclerosis and tumor metastasis. Methods for measuring cell migration in vitro have been developed over the years. The most common method uses an artificial barrier (membrane) and usually requires manual counting of migrat...

Claims

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