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Apparatus for carrying out real-time PCR reactions

A technology of reaction area and light-emitting diodes, which can be used in measuring devices, laboratory equipment, and material analysis through optical means, and can solve problems such as expensive

Inactive Publication Date: 2008-09-24
EPPENDORF AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The device is relatively expensive

Method used

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  • Apparatus for carrying out real-time PCR reactions

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Embodiment Construction

[0031] The device 10 comprises a schematically shown conventional thermal cycler 11 with a receptacle 12 . In operation, a reactor, not shown, is placed in the container 12, wherein the PCR filler with the above-mentioned fluorescent indicator or indicator is contained in the reactor.

[0032] A housing 13 with lighting with a plurality of light-emitting diodes 14 is arranged on the thermal cycler 11 . One light emitting diode 14 corresponds to one receptacle 12 . Preferably, the light emitting diodes 14 are arranged in an array. During the measurement, the light-emitting diodes 14 are preferably switched on and off in such a way that only the corresponding receptacle 12 is always illuminated.

[0033] Exemplary optical path lengths are shown at 15, 15'. Light 15 emerges from light-emitting diodes 14 and first passes through a short-pass filter 16 , by means of which the long-wave components are to be filtered out. The light 15 then passes through a beam splitter 17, which...

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Abstract

Apparatus for carrying out real-time PCR reactions, comprising a thermocycler having a reaction region with a plurality of temperature-regulable receptacles for reaction vessels, comprising an illumination device, which has a plurality of light-emitting diodes and is assigned to the reaction region and by means of which excitation light can be radiated into the receptacles, comprising a detector device, which generates measured values in a manner dependent on a measured light intensity, comprising optical devices defining a beam path that leads from the illumination device to the receptacles and from there to the detector device, comprising a reference device, which generates a reference measured value by measurement of the light intensity of a light-emitting diode, and comprising an evaluation device, which takes into account the reference measured value with the measured values,; wherein the reference device has a reference light-emitting diode, the light of which is coupled into the beam path behind the reaction region.

Description

technical field [0001] The invention relates to a device according to the preamble of claim 1 . Background technique [0002] Devices of this type are used to perform nucleic acid amplification methods (Nucleinsaeureamplfizierungsverfahren) (hereinafter referred to as PCR reactions), in which the formation of amplification products (PCR products) during the PCR reaction is measured optically. This particular form of PCR is called a real-time PCR reaction. [0003] In real-time PCR reactions, test substances are usually measured which comprise fluorescent indicators which, after excitation, emit a fluorescent signal whose intensity depends on the amount of PCR product formed. Typically, for real-time PCR reactions, the increase in PCR product can be tracked by an increase in the intensity of the measured fluorescent signal over the duration of the reaction. [0004] A known example of a suitable fluorescent indicator is, for example, the dye SYBR green (Sybrgreen), which is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/25G01N21/27
CPCG01N21/274G01N21/6452G01N21/253B01L7/525
Inventor 安德烈亚斯·斯尔格尔德·艾科特马库斯·莱普克兹纳
Owner EPPENDORF AG