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Color and shape compositely encoded hydrogel multi-component immune detection method

An immunological detection method and composite coding technology, which is applied in the field of multiple immunological detection of photonic crystal coding, can solve the problems of high cost and small coding amount, and achieve the effect of increasing the amount of coding, increasing the amount of coding, and high hydrophilicity

Inactive Publication Date: 2008-12-03
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Technical problem: Aiming at the disadvantages of high cost and small coding amount of the existing multiplex detection technology, the present invention provides a hydrogel multiplex immunoassay method coded by color and shape, which is stable when used in multiplex immunoassay High, large amount of coding, the advantages of effectively maintaining protein activity

Method used

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  • Color and shape compositely encoded hydrogel multi-component immune detection method
  • Color and shape compositely encoded hydrogel multi-component immune detection method
  • Color and shape compositely encoded hydrogel multi-component immune detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A hydrogel multiplex immunoassay method coded by color and shape, the detection steps are:

[0033] The first step is to prepare photonic crystal hydrogel films of various shapes and colors: Dilute non-close-packed silica colloidal crystals with a mass-volume ratio of 10% to a mass-volume ratio of 10% with a mixture of acrylamide and methylenebisacrylamide. A group of solutions with a volume ratio of 3% and 4% with different concentrations are added to the photoinitiator, the air is discharged, and poured into the mold, and the molds of the photonic crystal gel polymerization precursor solutions with different concentrations are sequentially covered with triangles. , round and square masks, let stand for 20 minutes, and then irradiate and polymerize with ultraviolet light for 60 minutes to obtain photonic crystal hydrogel films of different shapes and colors, and peel off the obtained photonic crystal hydrogel films from the mold. Store in pure water after washing;

[...

Embodiment 2

[0037] Detection of AIDS virus (HIV) and hepatitis B virus (HBV) in blood using photonic crystal hydrogel films:

[0038] (1) On the triangular and circular photonic crystal hydrogel films of the same color, HIV synthetic peptide antigen and hepatitis B surface antigen were respectively coated, and the triangular photonic crystals with different colors from the first two hydrogel films The crystalline hydrogel film was coated with bovine serum albumin as a control. The three coated coded hydrogel films were washed twice with phosphate buffered saline (PBS), and then blocked by adding bovine serum albumin (BSA) with a mass-volume ratio of 1%. After washing twice with phosphate buffered saline (PBS), the three coated coding hydrogel films were mixed to obtain multi-component immune hydrogel films with different specificities.

[0039] (2) Add the mixed three coded hydrogel films to 100ul serum to be tested, incubate at 37°C for 30 minutes, absorb the reaction solution, wash twi...

Embodiment 3

[0042] TORCH (TORCH refers to the pathogen that can cause congenital intrauterine infection and perinatal infection and cause perinatal malformation, it is the English name abbreviation of a group of pathogenic microorganisms, where T (Toxopasma) is Toxoplasma gondii, R (Rubella.Virus) is rubella virus, C (Cytomegalo.Virus) is giant cell, H (Herpes.Virus) is herpes simplex type I / II.) Diagnosis:

[0043] (1) Wrap T.gondii, Rubella virus, CMV, HSV Type II antigen and control bovine serum albumin on triangular or square photonic crystal hydrogel films of 5 different colors, and use phosphate buffered saline (PBS) Wash twice, add bovine serum albumin (BSA) with a mass volume ratio of 1% to block, wash twice with phosphate buffer saline (PBS), and mix 5 kinds of coated coding hydrogel films to obtain different specificities multi-component immune hydrogel films.

[0044] (2) Add the mixed 5 kinds of coded hydrogel films to 100ul serum to be tested, incubate at 37°C for 30 minutes...

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Abstract

The invention discloses a diversified immune detection method using hydrogel compositely coded by colors and shapes. The method adopts a photonic crystal hydrogel membrane with a code to ensure that an antibody or an antigen is combined with the photonic crystal hydrogel membrane; the category of the antibody or the antigen is marked by the code of the photonic crystal hydrogel membrane; through various antibodies or antigens marked by utilizing the photonic crystal hydrogel membrane, the corresponding antibody or the corresponding antigen in a same sample to be detected are detected simultaneously; the hydrogel membrane is encoded by utilizing the specific light reflection peak and different shapes of the photonic crystal; different antigens or antibodies are fixed on the surfaces of the hydrogel membranes with different codes; a plurality of hydrogel membranes with different codes are mixed with the same sample to be tested; and a plurality of objects to be analyzed in the sample can be tested simultaneously. The diversified immune analysis method based on the photonic crystal hydrogel membrane has the advantages of high sensitivity, large detection flux, simple carrier decoding, low detection cost, etc.

Description

technical field [0001] The invention relates to the field of clinical detection, in particular to a multiplex immune detection method encoded by photonic crystals. Background technique [0002] With the decoding of the human genome sequence, the function of genes and the interaction between the proteins encoded by genes have become research hotspots, and human beings have entered the post-genome era. A notable feature of the post-genome era is to study a large number of interactions between proteins and nucleic acids, proteins and proteins, and proteins and drugs. The study of these interactions is almost impossible with traditional experimental methods. Therefore, a high-throughput test platform is needed. The high-throughput test platform requires a high-throughput molecular carrier to identify the different interactions of different molecules, which is the so-called encoding process. Traditional methods, such as array biochips, use the fixed positions of molecules, that...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/532
Inventor 赵祥伟扈靖赵远锦李娟赵文举顾忠泽
Owner SOUTHEAST UNIV
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