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A kind of magnetic content coded polymer carrier microsphere and its preparation method

A polymer and polymer solution technology, which is applied in the preparation of microspheres, magnetic objects, microcapsule preparations, etc., can solve the problems of indistinguishable coding signals from detection signals, light fading, etc., and achieve low cost, large coding volume, and translation The effect of code simplicity

Active Publication Date: 2019-03-12
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are problems such as indistinguishability between the encoded signal and the detected signal, photofading and biocompatibility when using fluorescent encoding.

Method used

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  • A kind of magnetic content coded polymer carrier microsphere and its preparation method
  • A kind of magnetic content coded polymer carrier microsphere and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 Preparation of three differently coded magnetic polystyrene carrier microspheres:

[0035] 1. Polystyrene is dissolved in toluene to form a toluene solution of polystyrene, wherein the concentration of polystyrene in the solution is 0.2 g / mL.

[0036] 2. Add iron ferric oxide particles with an average particle size of 7nm to the polystyrene solution at a ratio of 20mg / mL, 40mg / mL, and 60mg / mL, and disperse evenly, and configure three different magnetic nanoparticle contents polystyrene toluene solution.

[0037] 3. Inject 3% polyvinyl alcohol solution into the three-way inlet A of the droplet generation device through a syringe pump, and inject a polystyrene toluene solution with a magnetic nanoparticle content of 20 mg / mL into inlet B to adjust the flow rate of the two-phase solution Let it form a uniform drop, and then receive the drop with a 3% polyvinyl alcohol solution at the outlet of the tee. Polystyrene toluene droplets with magnetic nanoparticles...

Embodiment 2 10

[0039] Example 2 Preparation of ten different coded magnetic polystyrene carrier microspheres:

[0040] 1. Polystyrene is dissolved in toluene to form a toluene solution of polystyrene, wherein the concentration of polystyrene in the solution is 0.2 g / mL.

[0041] 2. Diferric iron tetroxide particles with an average particle size of 7nm were prepared at 10mg / mL, 20mg / mL, 30mg / mL, 40mg / mL, 50mg / mL, 60mg / mL, 70mg / mL, 80mg / mL, 90mg / mL mL, 100mg / mL ratio was added to the polystyrene solution, and dispersed evenly, and configured into three polystyrene toluene solutions with different magnetic nanoparticle contents.

[0042] 3. Inject 3% polyvinyl alcohol solution into the three-way inlet A of the droplet generating device through a syringe pump, and inject a polystyrene toluene solution with a magnetic nanoparticle content of 10 mg / mL into inlet B to adjust the flow rate of the two-phase solution Let it form a uniform drop, and then receive the drop with a 3% polyvinyl alcohol so...

Embodiment 3

[0044] Example 3 Preparation of three differently coded magnetic polymethyl methacrylate carrier microspheres:

[0045] 1. Polymethyl methacrylate is dissolved in N, and the DMF solution that forms polymethyl methacrylate in N-methyl formamide (DMF), wherein the concentration of polymethyl methacrylate in the solution is 0.2g / mL.

[0046] 2. Add iron ferric oxide particles with an average particle size of 7nm to the polymethyl methacrylate solution at the ratio of 20mg / mL, 40mg / mL, and 60mg / mL, and disperse evenly, and configure three different magnetic Nanoparticle content in PMMA DMF solution.

[0047] 3. Inject 500cst of silicone oil into the tee inlet A of the droplet generating device through a syringe pump, and inject a polymethyl methacrylate DMF solution with a magnetic nanoparticle content of 20mg / mL in the inlet B, and adjust the flow rate of the two-phase solution so that Form a uniform droplet, and then receive the droplet with 500cst of silicone oil at the outl...

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Abstract

The invention relates to a content coded polymer carrier microsphere and a preparation method thereof. The content coded polymer carrier microsphere can serve as a coded suspension carrier for high-flux multi-protein and gene analysis or drug screening. The content coded polymer carrier microsphere is characterized in that magnetic nano particles are coated in a polymer microsphere, and a carrier microsphere taking magnetic content as a characteristic code is obtained according to different amounts of the coated magnetic nano particles. The diameter of the polymer coded microsphere is 50-500mu m, and the polymer coded microsphere can serve as a carrier for high-flux biological analysis. The coding mode and the preparation method of the content coded polymer carrier microsphere are simple and efficient, and the content coded polymer carrier microsphere has the advantages of simple coding and decoding, good stability, high flux, low cost and the like.

Description

technical field [0001] The invention relates to a polymer carrier microsphere using the content of wrapped magnetic particles as a characteristic code and a preparation method thereof. Background technique [0002] With the rapid development of biomedicine, high-throughput biological analysis is required in the fields of disease detection and drug discovery. In high-throughput analysis, the biomolecular carrier must be encoded first, and the tracking and detection of the biomolecular screening process can be realized by tracking and identifying the reaction information on the encoded carrier. The traditional array biochip uses the difference in fixed molecular position, that is, the coordinates as codes to distinguish different biomolecules. However, since the coordinates of the array biochip can only be fixed in two-dimensional space, the reaction speed and its application. In contrast, the mobile encoding vector can be freely dispersed into the reaction system without be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L25/06C08L33/12C08K3/22B01J13/02H01F1/36
CPCB01J13/02C08K3/22C08K2003/2275C08K2201/01H01F1/344H01F1/36C08L25/06C08L33/12
Inventor 谢卓颖陈姗赵远锦顾忠泽
Owner SOUTHEAST UNIV
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