Biological molecular detection method based on multilayer colloidal crystal

Abstract

The invention discloses a biological macromolecular detection method based on a multilayer colloidal crystal. The detection method is a simple and convenient high-flux detection method for performing biological macromolecular detection on a biological sample. In the biological molecular detection process, the biological macromolecular detection method taking a multilayer colloidal crystal film as a mobile carrier comprises the following steps of: 1, preparing a multilayer colloidal crystal film; 2, immobilizing a nucleic acid or protein probe molecule on the multilayer colloidal crystal film; 3, hybridizing target molecules; and 4, identifying the target molecules. The reflectance spectrum band of the multilayer colloidal crystal film is determined by utilizing a fiber optic spectrometer so as to identify varieties of bio-molecules, and whether the target molecules exist or not is determined according to the fluorescence signal or light absorbency on the target molecules. The method disclosed by the invention is high in hybridization speed and low in cost and is a simple and rapid high-flux biological detection method.

Description

technical field [0001] The invention is a simple and convenient high-throughput detection method for detecting biological macromolecules in biological samples. It can be widely used in clinical diagnosis and detection, drug screening, detection of microbial samples in the environment, forensic identification, customs inspection and quarantine of agricultural products import and export, gene sequence and functional analysis, microbial fractal, animal and plant breeding and other fields. Background technique [0002] With the advent of the genome era and the post-genome era, high-throughput gene detection and protein detection technologies have increasingly become the basic means and tools of this life science basic research. Parallel detection of high-throughput biomolecules can efficiently discover new functional genes and proteomes, and is widely used in the cloning, expression and proteome research of new functional genes, and in the development of new drugs, clinical test...

AI Technical Summary

Problems solved by technology

At present, in the detection of high-throughput biomacromolecules, it mainly relies on the position-encoded biochip with glass slides as the carrier and the liquid phase chip technology with fluorescent microspheres as the flow carrier, but both technologies have certain limitations. Disadvantages, although the biochip technology has a large amount of coding, it requires an expensive spotting system, and the hybridization speed is slow; while the liquid phase chip technology uses fluorescent microspheres as the carrier and adopts dynamic hybridization technology, but due to technical problems, the coding amount is small

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