Method for testing biomolecule based on colloid crystal

A colloidal crystal and detection method technology, which is applied in the field of high-throughput detection, can solve the problems of slow hybridization speed and the coding amount needs to be improved, and achieves the effects of reducing production cost, speeding up hybridization reaction speed and reducing detection cost.

Inactive Publication Date: 2005-11-16
NANJING DONGJIAN BIOLOGICAL TECH
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Problems solved by technology

At present, in the detection of high-throughput biomacromolecules, the array biochip technology based on position coding based on glass slides and the liquid phase chip technology using fluorescent microspheres as the flow carrier are mainly relied on, but both technologies exist. Certain defects, although the biochip technology has a large amount of coding, it requires an expensive spotting system and the hybridization speed is slow; while the liquid phase chip technology with fluorescent microspheres as the carrier greatly improves the hybridization rate due to the use of dynamic hybridization technology. speed
However, due to technical problems, the amount of coding needs to be improved

Method used

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  • Method for testing biomolecule based on colloid crystal
  • Method for testing biomolecule based on colloid crystal

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Embodiment Construction

[0028] figure 1 It is the preparation flow chart of the morphology coding and colloidal crystal mixed coding film and the structure diagram after the probe is fixed and hybridized. The first step is to infuse the bottom layer of PDMS1 on a glass or silicon wafer. After curing, it will be peeled off from the glass. The second step is the hydrophilization of the bottom layer of PDMS1. The third step is the colloidal crystal film on the hydrophilized bottom layer. PDMS1, the fourth step is photolithography to form a colloidal crystal film 2 with a specific morphology, the fifth step is the formation of the top PDMS film 3, and the sixth step is the bottom surface of the bottom PDMS film 1 and the top surface of the top PDMS film 3. Fix the probe 4, the seventh step is the hybridization of the target molecule 5 and the probe molecule.

[0029] figure 2It is a combination of shape coding and colloidal crystal barcode. Shape codes can be barcodes, Arabic numerals, English letters, va...

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Abstract

A method for detecting biological molecule based on colloid crystal includes preparing united code film of photon crystal and morphology, fixing probe molecule of nulein or protein, hybridizing target molecule, identifying target molecule ; using spectrograph to confirm reflecting spectrum and using microscope to observe film morphology, using integration of reflecting band and photoetched morphology as code to identify kind of biological molecule and confirming whether target molecule is existed or not according to fluorescent signal on target molecule.

Description

Technical field [0001] The invention is a simple and convenient high-throughput detection method for detecting biological samples. It can be widely used in clinical diagnosis and testing, drug screening, environmental microbial sample testing, forensic identification, customs agricultural import and export inspection and quarantine, gene sequence and function analysis, microbial fractal, animal and plant breeding and other fields. Background technique [0002] With the advent of the genomic era and the post-genomic era, high-throughput genetic testing and protein detection technologies have increasingly become the basic methods and tools of this basic life science research. Parallel detection of high-throughput biomolecules can efficiently discover new functional genes and proteomes. It has been widely used in the cloning, expression and proteomics research of new functional genes, and it has broad application prospects in the development of new drugs, clinical testing, and anima...

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Application Information

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IPC IPC(8): G01N21/00
Inventor 顾忠泽刘兆斌
Owner NANJING DONGJIAN BIOLOGICAL TECH
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