Method for testing biomolecule based on colloid crystal

The technology of a colloidal crystal and a detection method, which is applied in the field of high-throughput detection, can solve the problems such as the amount of coding needs to be increased, the hybridization speed is slow, etc., and achieve the effects of accelerating the hybridization reaction speed, reducing the production cost, and the method being simple and easy to implement.

Inactive Publication Date: 2008-12-24
NANJING DONGJIAN BIOLOGICAL TECH
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Problems solved by technology

At present, in the detection of high-throughput biomacromolecules, the array biochip technology based on position coding based on glass slides and the liquid phase chip technology using fluorescent microspheres as the flow carrier are mainly relied on, but both technologies exist. Certain defects, although the biochip technology has a large amount of coding, it requires an expensive spotting system and the hybridization speed is slow; while the liquid phase chip technology with fluorescent microspheres as the carrier greatly improves the hybridization rate due to the use of dynamic hybridization technology. speed
However, due to technical problems, the amount of coding needs to be improved

Method used

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  • Method for testing biomolecule based on colloid crystal
  • Method for testing biomolecule based on colloid crystal

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Embodiment Construction

[0029] figure 1 It is the flow chart of the preparation of the morphology coding and colloidal crystal mixed coding membrane and the structure diagram after probe immobilization and hybridization. The first step is the pouring of the underlying PDMS 1 on the glass or silicon wafer. After curing, it is peeled off from the glass sheet. The second step is the hydrophilization of the underlying PDMS1. The third step is on the hydrophilized underlying PDMS1. Self-assembled colloidal crystal film, the fourth step is to form a colloidal crystal film 2 with a specific morphology by photolithography, the fifth step is the formation of the top PDMS film 3, and the sixth step is to form the bottom surface of the bottom PDMS film 1 and the top PDMS film The upper surface of 3 is immobilized with probe 4, and the seventh step is the hybridization of target molecule 5 and probe molecule.

[0030] figure 2It is several forms of joint coding of shape coding and colloidal crystal barcode. ...

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Abstract

A method for detecting biological molecule based on colloid crystal includes preparing united code film of photon crystal and morphology, fixing probe molecule of nulein or protein, hybridizing target molecule, identifying target molecule ; using spectrograph to confirm reflecting spectrum and using microscope to observe film morphology, using integration of reflecting band and photoetched morphology as code to identify kind of biological molecule and confirming whether target molecule is existed or not according to fluorescent signal on target molecule.

Description

technical field [0001] The invention is a simple and convenient high-throughput detection method used for detection in biological samples. It can be widely used in clinical diagnosis and detection, drug screening, detection of microbial samples in the environment, forensic identification, customs inspection and quarantine of agricultural products import and export, gene sequence and functional analysis, microbial fractal, animal and plant breeding and other fields. Background technique [0002] With the advent of the genome era and the post-genome era, high-throughput gene detection and protein detection technologies have increasingly become the basic means and tools of this life science basic research. Parallel detection of high-throughput biomolecules can efficiently discover new functional genes and proteomes. It has been widely used in the cloning, expression and proteome research of new functional genes, and has broad application prospects in the development of new dru...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/544G01N21/27G01N21/64C12Q1/68G01N21/00
Inventor 顾忠泽刘兆斌
Owner NANJING DONGJIAN BIOLOGICAL TECH
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