Multilayer colloid crystal based biomolecule detecting method

Abstract

Biomolecule's detection method based on the multilayered colloid crystal carries on the biology macro-molecule examination is one kind of simple, convenience high flux examination method, in the examination process: First step, multilayered colloid crystal membrane and preparation; Second step, nucleic acid or protein probe molecular are fixed on multilayered colloid crystal membrane; Third step, goal molecular hybrid; Fourth step, goal molecular recognition. Using the optical fiber spectroscope to determine multilayer colloid crystal membrane reflection band to recognize biology molecular type, then on the basis of goal molecular fluorescence signal or light absorption degree sets a target molecular existence or not. This invention hybrid speed is high, the cost is low, which is one kind of simple, convenient and high flux examination method.

Description

technical field [0001] The invention is a simple and convenient high-throughput detection method for detecting biological macromolecules in biological samples. It can be widely used in clinical diagnosis and detection, drug screening, detection of microbial samples in the environment, forensic identification, customs inspection and quarantine of agricultural products import and export, gene sequence and functional analysis, microbial fractal, animal and plant breeding and other fields. Background technique [0002] With the advent of the genome era and the post-genome era, high-throughput gene detection and protein detection technologies have increasingly become the basic means and tools of this life science basic research. Parallel detection of high-throughput biomolecules can efficiently discover new functional genes and proteomes, and is widely used in the cloning, expression and proteome research of new functional genes, and in the development of new drugs, clinical test...

AI Technical Summary

Problems solved by technology

At present, in the detection of high-throughput biomacromolecules, it mainly relies on the position-encoded biochip with glass slides as the carrier and the liquid phase chip technology with fluorescent microspheres as the flow carrier, but both technologies have certain limitations. Disadvantages, although the biochip technology has a large amount of coding, it requires an expensive spotting system, and the hybridization speed is slow; while the liquid phase chip technology uses fluorescent microspheres as the carrier and adopts dynamic hybridization technology, but due to technical problems, the coding amount is small

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