Quantum dot for fluorescent lifetime codes and fluorescent lifetime coding method thereof

Abstract

The invention provides a quantum dot for fluorescent lifetime codes and further provides a target fluorescent lifetime coding method of the quantum dot.The quantum dot comprises at least two kinds of target quantum dots with different fluorescent lifetimes.Each target quantum dot comprises a cadmium telluride / cadmium sulfide quantum dot and cell-penetrating peptide used for recognizing cancer cells, the cell-penetrating peptide and the cadmium telluride / cadmium sulfide quantum dots are connected through coordination, and each cadmium telluride / cadmium sulfide quantum dot has a core-shell structure with cadmium telluride as a core and cadmium sulfide as a shell.The different fluorescent lifetime codes can be obtained by mixing the target quantum dots with the different lifetimes and changing the proportion of the target quantum dots, the quantum dots with the different lifetimes and for the fluorescent lifetime codes can produce a large number of different differentiable optical signals, and the coding information content is large.The quantum dot for the fluorescent lifetime codes can serve as fluorescence indicators to be widely applied to living imaging study.

Description

technical field [0001] The invention belongs to the technical field of biological analysis, in particular to a quantum dot used for fluorescence lifetime coding and a fluorescence lifetime coding method thereof. Background technique [0002] Existing live cell fluorescence imaging mostly uses organic dyes to achieve fluorescence microscopy. Organic dyes have a high degree of commercialization and a variety of categories, and can target different organelles for fluorescence imaging. However, the fluorescence intensity of organic dyes is dependent on laser intensity and dye concentration. In addition, organic dyes have disadvantages such as low quantum yield, relatively narrow absorption and emission, poor resistance to photobleaching, and short fluorescence lifetime. [0003] Semiconductor quantum dots (referred to as QDs, also known as semiconductor nanocrystals) have attracted the attention of researchers in the fields of photoelectric conversion and life science due to the...

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Problems solved by technology

However, the fluorescence intensity of organic dyes is dependent on laser intensity and dye concentration. In addition, organic dyes have disadvantages such as low quantum yield, relatively narrow absorption and emission, poor resistance to photobleaching, and short fluorescence lifetime.

[0003] Semiconductor quantum dots (referred to as QDs, also known as semiconductor nanocrystals) have attracted the attention of researchers in the fields of photoelectric conversion and life science due to their unique optical and electrical properties. Target recognition of a single cell in the environment. In addition, the existing quantum dots generally use the change of fluorescence intensity to encode living cells, but because the fluorescence intensity changes with the change of the concentration of quantum dots, the fluorescence intensity is unstable and difficult to perform Specific coding; at the same time, the existing near-infrared coding capacity of quantum dots is small, and it is difficult to realize simultaneous coding of multiple living cells or multiple biomolecules

These defects greatly limit the application of quantum dots in the biological field

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