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Greater wax moth feedstuff additive and feed containing the additive agent

A kind of feed additive, the technology of Galleria mellonella, which is applied in the field of Galleria mellonella feed and achieves the effect of low cost

Inactive Publication Date: 2008-12-17
GUANGDONG INST OF APPLIED BIOLOGICAL RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the use of Huaxiangworm tea as a feed additive for the greater wax moth

Method used

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  • Greater wax moth feedstuff additive and feed containing the additive agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Grind 100g of Huaxiangchong tea, soak in 2200mL of 95% ethanol by volume at 35°C for 24 hours, filter, collect the filtrate and filter residue respectively, add 2200mL of 95% ethanol by volume to the filter residue and soak at room temperature for 2 days, and collect the filtrate by filtration. The filtrates from the two soaks were mixed, and the ethanol was evaporated to obtain 500 mL of ethanol extraction concentrate of Huaxiangchong tea.

[0021] Add 800mL of distilled water and 800mL of petroleum ether to the ethanol extraction concentrate of Huaxiangchongcha, discard the petroleum ether layer after extraction, and discard the remaining chloroform layer after extraction with 800mL of ethyl acetate. The ethyl acetate layer was removed, and the rest was extracted with 800 mL of n-butanol, and the n-butanol layer was discarded to obtain a feed additive.

Embodiment 2

[0023] Mix 100g corn flour, 100g flour, 100g rice bran, 100g glycerin, 100g honey, 100g milk powder, 150kg beeswax, 0.25g complex vitamin B and 1.85g yeast flakes to obtain 752g of mealworm larvae feed, add the feed obtained in Example 1 Add 6mL of additives, mix well, and make the artificial diet of wax moth.

Embodiment 3

[0025] Get the wax moth artificial feed 50g that embodiment 2 obtains, be divided into 3 parts on average, as experimental group. In addition, get 50 g of common S. mellonella feed that does not add feed additive of the present invention, and divide into 3 parts on average, as control group 1, get the commonly used S. mellonella feed 50 g that has added 0.4mL distilled water, and averagely divide into 3 parts, as control group 2. The feeds of the experimental group and the control group were put into the feeding boxes with the wax moth respectively, and the 2nd instar larvae of the wax moth were picked out, and 10 wax moths were put in each feed, and the amount of wax moth in each feed was determined. After weighing the weight of the borer, the rearing box was covered with a ventilating lid and placed in an insect culture room at 25°C. And on the 7th day and the 14th day, the mortality rate, food intake, body weight change, etc. of the greater wax moth were measured respective...

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Abstract

The invention relates to a Galleria mellonella L. feed additive which is characterized in that platycarya strobilacea insert tea is immersed by using ethanol and concentrated, distilled water is added, petroleum ether, chloroform, ethyl acetate and n-butyl alcohol are sequentially extracted for preparation. The invention further relates to a Galleria mellonella L. feed containing the additive, the Galleria mellonella L. feed is characterized in that the total weight percentage is counted by 100 percent, and the Galleria mellonella L. feed is composed of 0.5 percent to 0.9 percent feed additive and 99.1 percent to 99.5 percent commonly used Galleria mellonella L. feed. The feed additive of the invention is safe and non-toxic on Galleria mellonella L. and has no adverse effects on Galleria mellonella L. larvae survival, pupation, pupae emergence, spawning of emerged adult and the like, the feed intake and the body weight of the Galleria mellonella L. larvae can be improved by 1.5 to 3.0 times compared with the commonly used feed for feeding by only adding a small amount into the commonly used Galleria mellonella L. feed.

Description

technical field [0001] The invention relates to a new feed additive, in particular to a feed additive for the greater wax moth extracted from huaxiang chrysanthemum tea, and also to a greater wax moth feed containing the additive. Background technique [0002] The greater wax moth (Galleria mellonella L.) is a pest that eats the honeycomb and destroys the honeycomb, but it is also an experimental insect for many types of biological experiments, such as the isolation and reproduction of nematodes and microorganisms, some active substances and toxic substances. The bioassay experiments of substances require the use of the greater wax moth. In addition, the greater wax moth can also be used as feed for animals such as fish, birds and insect natural enemies. Therefore, the cultivation of the greater wax moth is increasingly valued by people. Both are reported. However, the larvae of Melonella mellonella artificially cultivated at present are relatively small, and the cultivatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/00A23K10/30A23K20/00A23K20/10A23K40/00A23K50/90
Inventor 韩日畴张丽坤
Owner GUANGDONG INST OF APPLIED BIOLOGICAL RESOURCES
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