Construction method of rat hepatocirrhosis and osteoporosis animal model

Abstract

The invention relates to a method for constructing a rat model which has liver cirrhosis and osteoporosis. Corn meal, rat feed meal and dried hen egg yolk are mixed to get A. Cholesterol is put into molten lard to get B. B is put into A to be mixed to get C. Sodium deosycholate is dissolved by water and mixed with C. Afterwards, the water is added into mixture and the mixture takes sticky. The sticky mixture is baked and cooled to obtain model composite feed. 26 male SD rats at SPF level are selected and are randomly divided into two groups, group A and group B. The group A is for the model of the liver cirrhosis and the osteoporosis, so the rats in group A are fed with the model composite feed. In the first week, the rats in group A drink the ethanol water of 5 percent. In the second week, the rats in group A drink the ethanol water of 8 percent and sesame oil solution containing the carbon tetrachloride of 40 percent is applied to the rats by subcutaneous injection by 1.3ml/kg for every other five days. After 40 days, the ret model which has the liver cirrhosis and the osteoporosis is made. The group B is a normal group for reference, so the rats in group B is fed with usual feed and drink water. During 40 days, physiological saline is applied to the rats in group B by 1.3ml/kg for every other five days.

Description

technical field [0001] The invention belongs to the technical field of combining an animal model of liver cirrhosis with an animal model of osteoporosis, and in particular relates to a method for constructing an animal model of rat liver cirrhosis and osteoporosis. Background technique [0002] More and more studies suggest that liver cirrhosis is one of the high risk factors for osteoporosis (OP). The main reasons are as follows: (1) The liver plays a very important role in bone metabolism. Calcium in the basic structure of bones needs the assistance of vitamin D to be absorbed from the intestines, but vitamin D must first pass through the liver and kidneys. Hydroxylation is active and plays a physiological role. After liver cirrhosis, the hydroxylation (ie, "activation") function of vitamin D is significantly reduced, thereby significantly affecting calcium absorption, blood calcium stability, and bone calcium deposition. Most important is osteoblast dysfunction and decr...

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Problems solved by technology

(3) In addition to the decreased absorption of bone calcium, the digestive function of patients with liver cirrhosis is decreased, which will lead to insufficient intake of protein, trace elements, vitamin K, etc.

[0003] However, at present, a "bottleneck" problem that restricts the etiology, interaction mechanism, clinical prevention, basic research and new drug development of liver cirrhosis secondary osteoporosis (LC-OP) is the lack of suitable experimental animal models as research vehicles

At present, although there are some methods for establishing animal models of rat liver cirrhosis and osteoporosis in foreign countries, carbon tetrachloride is mainly used to induce the model, which has long modeling cycle, inconsistent administration cycle and intervention mode, and lack of consensus. Inclusion criteria for experimental animals and model evaluation criteria

At present, the mouse animal model is mainly used in China. The tolerance of experimental animals is poor, the accidental death rate is high, and the model time limit is short.

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