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Analogue model of skin with pigments for selecting stripping agent and construction method thereof

A construction method and analog technology, applied in the field of pigmented skin analogs and their construction, can solve the problems of large discrepancies in experimental results, and achieve the effect of good vitality, good condition and complete structure

Inactive Publication Date: 2009-01-21
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the differences in the cultured cell lines and culture conditions, the experimental results are quite different.

Method used

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  • Analogue model of skin with pigments for selecting stripping agent and construction method thereof
  • Analogue model of skin with pigments for selecting stripping agent and construction method thereof
  • Analogue model of skin with pigments for selecting stripping agent and construction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0038] 1. Cell Isolation and Culture

[0039] (1) Separation of specimens

[0040]The specimens of healthy people after circumcision (age 5-10 years old) were soaked in 0.25% chlorhexidine solution for 10 minutes, rinsed in 0.01M PBS buffer for 3 times, and sterilized with instruments on the ultra-clean table. Remove the subcutaneous tissue and meat membrane as much as possible, then cut the specimen into thin strips of 1mm×2mm, wash 3 times in PBS solution for 5min each time, transfer to 2.5g / L Dispase II enzyme, and cool overnight in the refrigerator at 4°C Digestion 16-22h.

[0041] (2) Culture and identification of fibroblasts

[0042] Take out the cold-digested tissue, separate the epidermis and dermis with ophthalmic forceps, add an appropriate amount of calf serum to the separated dermal tissue, and cut it into 1×1×1mm 3 size tissue block, put into 25cm 2 Place the flask upside down at 37°C, 5% CO 2 After 30 minutes, add a small amount of DMEM culture solution cont...

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PUM

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Abstract

The invention belongs to the biotechnology filed and relates to a pigmented skin equivalent used for screening decolorizing agents and a constructing method thereof. A dermic skin equivalent layer is constructed by skin fibroblasts that are cultured in vitro and collagen, and the equivalent is constructed after inoculating keratinocyte and melanocyte on the skin equivalent layer by using gas-liquid plane three-dimensional culture technology. The obtained pigmented skin equivalent that is similar to the structure of skin can imitate the transportation process of melanin bodies, be used for screening the decolorizing agents and provide a more scientific model for the study of melanocyte in vitro biology and the screening of decolorizing agents and whitening cosmetics.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a pigmented skin analog that can be used for screening fading agents and a construction method thereof. Background technique [0002] Skin grafts are often used in plastic surgery to repair large areas of skin defects. The color of the transplanted area and the surrounding skin is often different after surgery, and the pigmentation is usually increased. In recent years, due to the destruction of the environment and the ozone layer caused by pollution, the metabolic disorder of melanin-forming enzymes has occurred, and the number of patients suffering from various dark spot symptoms such as freckles, chloasma, and age spots has increased. Therefore, the treatment of hyperpigmentation and other pigmented diseases has high social and economic benefits. How to solve the problem of pigmentation has become an important research topic for plastic surgeons and dermatologists. [0003] Mature...

Claims

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Application Information

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IPC IPC(8): A61L27/60A61L27/38
Inventor 杨壮群王正辉张铁良屠军波荔鹏
Owner XI AN JIAOTONG UNIV
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