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Reagent for latex aggregation reaction and method for detecting target substance

A technology for detecting targets and target substances, applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as differences in detection results and poor detection accuracy, and achieve the effects of improved reproducibility and accuracy, small deterioration, and easy operation

Active Publication Date: 2014-04-16
JSR CORPORATIOON
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is a problem of differences in detection results or poor detection accuracy

Method used

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  • Reagent for latex aggregation reaction and method for detecting target substance
  • Reagent for latex aggregation reaction and method for detecting target substance
  • Reagent for latex aggregation reaction and method for detecting target substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Embodiment 1 (synthesis of polyacrylamide)

[0095] 100 g of acrylamide and 2 g of cysteamine hydrochloride as a chain transfer agent were mixed in 900 g of water. The resulting mixture was placed in a separable flask equipped with a stirrer and heated to 70°C under a nitrogen purge. After adding 1 g of 2,2'-azobis(2-methylpropionamidine) dihydrochloride as an initiator, polymerization was carried out for 2 hours. Then heated to 80° C. for aging for 3 hours, and then cooled to room temperature. The obtained aqueous solution was purified by dialysis to obtain a 6% aqueous solution of polyacrylamide. The weight average molecular weight (Mw) measured by GPC of the obtained polyacrylamide was 46,600, and Mw / Mn was 1.9.

Embodiment 2

[0096] Embodiment 2 (the synthesis of dimethylacrylamide-methoxy ethyl acrylate copolymer)

[0097] By the same method as in Example 1, a 6% aqueous solution of dimethylacrylamide-methoxyethylacrylate copolymer was obtained, except that 80 g of dimethylacrylamide and 20 g of methoxyethylacrylate were replaced 100 g of acrylamide and 4 g of cysteamine hydrochloride instead of 2 g of cysteamine hydrochloride. The weight average molecular weight (Mw) of the obtained copolymer measured by GPC was 23,600, and Mw / Mn was 2.4.

Embodiment 3

[0098] Embodiment 3 (immobilization of antibodies on latex particles)

[0099] Disperse 1 mL of a 10% solution of latex particles for immunodiagnosis (product number: coat G0305 (average particle diameter: 0.31 μm, surface COOH group content: 0.21 mmol / g-LTx, produced by JSR Co., Ltd.)) to adjust the particle concentration to 1% by mass.

[0100] Then, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (Dojindo Laboratories, hereinafter referred to as WSC) was added to adjust the final concentration to 0.05%. Add an equal amount of 1 mg / mL solution of anti-CRP (C-reactive protein) antibody (rabbit) to the particle dispersion, and immobilize the antibody (anti-CRP antibody) to the particle surface. Then, 0.5 mL of 1% bovine serum albumin / 0.05% Rapigest SF was added to the particle dispersion, followed by stirring with slow rotation at room temperature for 10 hours. After these operations, the particle dispersion was transferred to a centrifuge tube, centrifuged at ...

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Abstract

A reagent for latex aggregation reaction contains a water-soluble polymer having a repeating unit represented by the following formula (1): wherein R 1 and R 2 each independently represents a hydrogen atom or a substituted or unsubstituted alkyl group having 1 to 8 carbon atoms.

Description

technical field [0001] The invention relates to a reagent for latex aggregation reaction and a method for detecting target substances. Background technique [0002] In order to detect specific antigens or antibodies (target substances) through antigen-antibody aggregation reactions, immunological latex aggregation assays are widely used in clinical laboratory testing, biochemical research, etc., which load immunologically active substances such as antibodies or antigens on on the carrier particles, and detect target substances such as corresponding antigens or antibodies through specific reactions (eg JP-A-2006-266970). [0003] In the immunolatex aggregation detection method, generally, the carrier particle of the antibody (antigen) immobilized with the target antigen (antibody) to be tested is contacted with the analyte that may contain the target substance, and when the target substance is present, it can be detected. - Aggregation of particles in mixed liquids caused by...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N21/17
CPCG01N33/531G01N33/54313C08F220/26C08F220/56G01N33/54393
Inventor 范可君田守功二
Owner JSR CORPORATIOON
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