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Neurotrophin-derived peptide sequences

一种神经营养因子、肽序列的技术，应用在神经营养因子衍生的肽序列领域

Inactive Publication Date: 2009-02-04
UNIVERSITY OF COPENHAGEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, they were again reported to induce a maximal survival response of only 30% of that elicited by natural neurotrophic factors (O'Leary et al., J. Biol. Chem. 2003, 278:25738-25744)

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0216] The preparation of polyclonal antibodies is well known to those skilled in the art. See, for example, Green et al., 1992, Production of Polyclonal Antisera, in Immunochemical Protocols (Manson ed.), pp. 1-5 (Humana Press); Coligan et al., Production of Polyclonal Antisera in Rabbits, Rats, Mice and Hamsters, in Current Protocols in Immunology , Section 2.4.1, which are incorporated herein by reference.

[0217] The preparation of monoclonal antibodies is likewise routine. See, eg, Kohler and Milstein, Nature, 256:495-7 (1975); Coligan et al., Sections 2.5.1-2.6.7; and Harlow et al., Antibodies: A Laboratory Manual, page 726, Cold Spring Harbor Pub. (1988) . Monoclonal antibodies can be isolated and purified from hybridoma cultures by a variety of well-established techniques. These separation techniques include affinity chromatography using Protein A-Sepharose, size exclusion chromatography, and ion exchange chromatography. See eg Coligan et al., Sections 2.7.1-2.7.1...

Embodiment 1

[0286] Example 1: Stimulation of neurite outgrowth

[0287] CGN prepared as above were treated with peptides NGF-C2, NGF-D, NGF-E, NGF-C1, NGF-EE, BDNF-E or BDNF-EE at different concentrations (3-81 μg / ml).

[0288] From figure 1 , 2 , 4 and 5, it can be seen that the peptides NGF-C2 (motif II), NGF-E (motif III), NGF-C1 (motif I), NGF-EE (motif III) containing the specified structural motifs , BDNF-E (motif III) or BDNF-EE (motif III) all significantly stimulated neurite outgrowth from primary neurons in culture. In contrast, the peptide NGF-D, which lacks the essential hydrophobic residues of motif I, does not have neuritic activity ( figure 1 ).

Embodiment 2

[0289] Example 2: Stimulation of neuronal cell survival

[0290] The effect of different peptides on neuronal survival was examined both in vivo and in vitro.

[0291] For in vitro experiments, cultures of CGR neurons were prepared as above and treated with different concentrations of NGF-E, NGF-EE or NGF-N.

[0292] From image 3 As can be seen, all tested peptides significantly promote the survival of CGN in culture.

[0293]In vivo test - Focal brain injury of the right parietofrontal cortex by applying a piece of dry ice (-78°C) directly to the skull of a mouse for 30 seconds and to a rat skull for 60 seconds, as previously described in detail (Penkowa et al., 2003). Focal brain injury of the right fronto-parietal cortex was induced by applying a piece of dry ice (-78°C) directly to the mouse skull for 30 s and to the rat skull for 60 s, as previously described. described in detail (Penkowa et al., 2003). Following treatment, sections were incubated overnight at 5°C w...

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Abstract

The present invention relates to peptide sequences capable of stimulating neuronal cell differentiation, neural cell survival and neuronal plasticity associated with memory and learning. The peptide sequences of the invention are derived from the proteins belonging to neurotrophic factors, such as NGF, NT3, NT4 / 5 and BDNF. The invention also relates to pharmaceutical compositions comprising said peptide fragments and uses thereof for treatment of a disease or condition wherein the effects of stimulating neuronal cell differentiation, neuronal cell survival, stimulating neural plasticity associated with learning and memory are beneficial for treatment.

Description

field of invention [0001] The present invention relates to peptide sequences capable of stimulating neuronal cell differentiation, neuronal cell survival, and neuronal plasticity related to memory and learning. The peptide sequences of the present invention are derived from proteins belonging to the neurotrophic factors, such as NGF, NT3, NT4 / 5 and BDNF. The present invention also relates to pharmaceutical compositions comprising said peptide fragments and their use in the treatment of diseases or disorders in which the effects of stimulating neuronal cell differentiation, neuronal cell survival, and neuroplasticity related to learning and memory are therapeutically beneficial. Background of the invention [0002] The growth, maintenance and regeneration of neurons are regulated at least in part by certain polypeptide growth factors called neurotrophins (NT), which bind to and activate cell surface receptors of the Trk family, which The cell surface receptor has intrinsic t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K4/12
CPCC07K14/475A61P25/00A61P25/14A61P25/16A61P25/18A61P25/28A61P25/32A61P35/00A61P43/00
Inventor 伊丽莎白·博克弗拉迪米尔·贝里津
Owner UNIVERSITY OF COPENHAGEN
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