Method for coculturing fine algae and photosynthetic bacteria

A technology of photosynthetic bacteria and co-cultivation, applied in the biological field, can solve problems such as unseen research reports, and achieve the effect of high biomass

Inactive Publication Date: 2009-02-11
广东绿百多生物科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Although there are a large number of reports on the cultivation of photosynthetic bacteria or microalgae, how to use the respective physiological and ecological characteristics of photos

Method used

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  • Method for coculturing fine algae and photosynthetic bacteria
  • Method for coculturing fine algae and photosynthetic bacteria
  • Method for coculturing fine algae and photosynthetic bacteria

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Embodiment Construction

[0018] First, a glass bottle with a volume of 5 liters was used to prepare 4.5 liters of liquid culture medium according to the average value of each component of the culture medium using tap water. The culture medium that embodiment 1 adopts consists of (g / L): MgSO 4 0.6, NaHCO 3 2.0, CaCl 2 0.015, NH 4 Cl 2.0, KH 2 PO 4 1.5, NaCl 3.0, yeast extract 0.6, sodium acetate 3.5, trace element solution 1.0ml / L. The culture medium that embodiment 2 adopts consists of (g / L): MgSO 4 7H 2 O 0.2, NaHCO 3 1.0, CaCl 20.01, NH 4 Cl 1.0, KH 2 PO 4 1.0, NaCl 1.0, yeast extract 0.2, sodium acetate 2.0, trace element solution 1.0ml / L. The culture medium that embodiment 3 adopts consists of (g / L): MgSO 4 7H 2 O 1.0, NaHCO 3 3.0, CaCl 2 0.02, NH 4 Cl 3.0, KH 2 PO 4 2.0, NaCl 5.0, yeast extract 1.0, sodium acetate 5.0, trace element solution 1.0ml / L. Trace element solution includes (mg / L): FeCl 3 6H 2 O 5.0, CuSO 4 5H 2 O 0.05, H 3 BO 3 1.0, MnCl 2 4H 2 O 0...

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Abstract

The invention provides a co-culture method of micro-algae and photosynthetic bacteria, and the co-culture method belongs to the field of biotechnology. The co-culture method is characterized in that: the co-culture method adopts a translucent glass or plastic container as the container and adopts tap water for preparing a liquid culture medium, the liquid culture medium is composed of 0.2 to 1.0g/L of MgSO47H2O, 1.0 to 3.0g/L of NaHCO3, 0.01 to 0.02g/L of CaCl2, 1.0 to 3.0g/L of NH4Cl, 1.0 to 2.0g/L of KH2PO4, 1.0 to 5.0g/L of NaCl, 0.2 to 1.0g/L of yeast extract, 2.0 to 5.0g/L of sodium acetate and 1.0ml/L of trace element liquid. The micro-algae and the photosynthetic bacteria are co-cultured at the average temperature of larger than 20 DEG C and under the solar radiation after the inoculation, the micro-algae and the photosynthetic bacteria with the high cell concentration can be obtained after 5 days, the obtained biomass of the micro-algae or the biomass of the photosynthetic bacteria can be respectively improved by 65 percent or 100 percent compared with the single culture, thereby realizing the purpose of high efficient co-culture of the micro-algae and the photosynthetic bacteria, and an algae bacteria culture which is obtained by culture can be applied as a water quality purifier and a feed additive for the aquaculture.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular provides a method for co-cultivating microalgae and photosynthetic bacteria. Background technique [0002] Microalgae belong to lower plants and are simple photosynthetic organisms. Chlorella is a single-celled green alga of the genus Chlorella of Chlorphyta. There are about 10 species. Common Chlorella species in my country include Chlorella ellipsoides, Chlorella vulgaris and pyrenoids algae etc. Chlorella is rich in protein, unsaturated fatty acids, carotenoids, lutein, astaxanthin and multivitamins, and has extremely high nutritional value and the function of improving immunity. In addition, the Chlorella Growth Factor in Chlorella not only induces interferon to activate immune cells, but also promotes the body's detoxification and excretion of harmful substances, and can promote the growth of brewer's yeast. At present, Chlorella is not only a good model organism in the study...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/20C12R1/89C12R1/01
Inventor 张金燕徐中文周绍生张兴锋张璐许荣花孙建新许倩倩闫海
Owner 广东绿百多生物科技有限公司
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