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Immune chromatography reagent kit for screening multiple great infectious diseases simultaneously

A technology of immunochromatography and reagent kits, which is applied in the direction of analysis materials, measuring devices, instruments, etc., can solve the problems of long detection time, waste of manpower and material resources, inconvenient and fast, etc., and achieve the effect of shortening the window period and saving costs

Inactive Publication Date: 2009-02-25
FAPON BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, when screening for physical examination, blood donation, blood transfusion, pre-operation and home self-examination, multiple separate tests are required, which is not convenient and fast enough, resulting in long detection time, low efficiency, high cost, and waste of manpower and material resources.

Method used

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  • Immune chromatography reagent kit for screening multiple great infectious diseases simultaneously

Examples

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Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Coating of Antigen and Antibody

[0056] Dilute HBsAg monoclonal antibody, HCV antigen, HIV antigen, HIV P24 monoclonal antibody, and TP antigen to appropriate concentrations, and draw them on the NC membrane with a film-drawing instrument, that is, HBV, HCV, HIV (including HIV P24Ag), TP Five detection lines. Dilute the quality control line antibody goat anti-rabbit IgG antibody to the optimum concentration and draw it on the NC membrane with a film-drawing instrument, which is the control line. Place the scratched NC film in a 37-degree incubator to dry for half an hour.

Embodiment 2

[0057] Example 2 Preparation of colloidal gold

[0058] Get 100ml of ultrapure water in a 500ml round bottom flask, put it on a heating stirrer and heat to boiling, add 1ml of 1% aqueous solution of chloroauric acid (Sigma-Aldrich, U.S.), add 0.5ml of 1% sodium citrate after boiling (U.S. Sigma-Aldrich Company), continue to heat for 10 minutes and place it to cool naturally, which is colloidal gold.

Embodiment 3

[0059] Example 3 Preparation of Colloidal Gold-Marker

[0060] Take 10ml of HBsAg monoclonal antibody and put it into a dialysis bag, dialyze against 10mM PBS pH 7.4 buffer solution for 8 hours, during which the buffer solution is changed every 1 hour. Take 10ml colloidal gold, with 0.2M K 2 CO 3 Adjust the pH of the colloidal gold solution to 6-7, add 200ug HBsAg monoclonal antibody, and continue stirring for 5-10 minutes. Add 0.5 ml of 2% BSA solution to the above mixture, and continue stirring for 5 minutes. Put the above mixture into a suitable centrifuge tube. Centrifuge at 5000rpm for 10 minutes, suck out the supernatant, and collect the precipitate into a suitable container; centrifuge the supernatant at 10000rpm for 15 minutes, repeat the first centrifugation operation; centrifuge the supernatant at 12000rpm for 30 minutes, discard the supernatant, Collect the three centrifuged precipitates into the same centrifuge tube, dilute to 1ml with colloidal gold diluent, a...

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Abstract

The invention relates to an immune chromatography kit used for simultaneously realizing screening of three or four of four major infectious diseases (AIDS, hepatitis B, hepatitis C and syphilis) by a single experiment. The invention has the characteristics as follows: the particle diameter of a colloidal gold is increased to 80 to 90nm from the typical 20 to 40nm, so that the sensitivity of the colloidal gold is improved; two enveloping modes are available, for instance, all test items are underlined on the same NC membrane with one separate from another, and assembled on the same test strip finally, or, for instance, all test items are separated and underlined on different NC membranes, and are arranged and assembled separately; and then, the envelope antigens / antibodies of all test items are mixed together and underlined, thereby resulting in only one test line. The invention has the advantages that the invention can be applied to screening prior to physical examination, blood donation, blood transfusion, surgery and the like, so that the test time can be saved, the efficiency can be improved, the HIV P24 antigens can be tested as early as possible, and the window period of HIV test can be shortened.

Description

technical field [0001] The present invention relates to an immunochromatography kit, in particular to an immunochromatography kit that can simultaneously screen three to four of the four major infectious diseases of AIDS, hepatitis B, hepatitis C, and syphilis through a single experiment. Chromatography kits. Background technique [0002] Due to the spread of infectious diseases, it is necessary to detect infectious diseases before physical examination, blood donation, blood transfusion, and surgery. At present, the most widely transmitted and most harmful infectious diseases are AIDS, hepatitis B, hepatitis C, and syphilis. Hepatitis B virus (HBV) surface antigen (HBsAg), hepatitis C virus (HCV) antibody (anti-HCV), human immunodeficiency virus (HIV) antibody (anti-HIV) and P24 antigen (HIV P24Ag), Treponema pallidum (TP ) antibody (anti-TP), that is, HBV, HCV, HIV, and TP detection, is currently the main item for infectious disease screening. [0003] At present, the mo...

Claims

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Application Information

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IPC IPC(8): G01N33/576G01N33/571G01N33/569
Inventor 孙兴宝孙婧胡洋何志强胡鹏李泓彦曹菲崔鹏
Owner FAPON BIOTECH INC
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