Gold nano-bar based medicine carrier and preparation technique and use thereof

Abstract

The invention discloses a gold nanorods based drug carrier. The carrier is characterized in that: gold nanorods is wrapped with a polyanion film layer, a polycation film layer and an hTERT gene antisense oligonucleotide layer from inside to outside. The gold nanorods based drug carrier is prepared by the layer-by-layer self-assembly principle by utilizing the electrostatic force among radicals, and can be used for preparing inhibitors for malignant tumor cell telomerase. The invention causes hTERT gene antisense oligonucleotide to be loaded on the gold nanorods for the first time to solve theproblem of low transfection rate occurred in naked antisense oligonucleotide. The prepared gold nanorods based drug carrier does not only has the advantages of high transfection rate, strong targeting property, obvious effect of inhibiting the activity of the malignant tumor cell telomerase, and the like, but also has the advantages of safety and innocuity, which opens up a new way for the application of the gold nanorods in the biomedicine field; in addition, the invention has the advantages of simple operation for the preparation technology and low cost, and is suitable for scale production.

Description

A gold nanorod-based drug carrier and its preparation method and application technical field The present invention relates to a gold nanorod-based drug carrier and its preparation method and application, specifically, to a gold nanorod-based drug carrier loaded with hTERT gene antisense oligonucleotide and its preparation method and apply. Background technique Nano-drug carrier technology uses nanoparticles as a drug-carrying tool, wraps drug molecules in nanoparticles or adsorbs on its surface, and introduces drugs into cells through endocytosis, so as to achieve effective and safe treatment. Compared with traditional zero-dimensional nanoparticles, the application of gold nanorods in drug carriers has the following advantages: 1) gold nanorods, especially functionalized gold nanorods, have good biocompatibility and low toxicity to biological systems ; 2) Gold nanorods have a high specific surface area, and modifying the biological system on the wall surface can increas...

AI Technical Summary

Problems solved by technology

Because successful gene therapy mainly depends on how to effectively introduce therapeutic genes with specific therapeutic effects into target cells, and therapeutic genes such as naked antisense oligonucleotides have low transfection efficiency and are easily detected by nucleases. Degradation and other issues

Therefore, how to develop a highly efficient, safe, and non-toxic drug carrier to effectively introduce therapeutic genes such as antisense oligonucleotides into target cells is of great significance for the realization of malignant tumor gene therapy, but so far there is little information about this type of technology The reports of human telomerase catalytic subunit (hTERT) gene are rare, especially the gold nanorod-based drug carrier loaded with hTERT gene antisense oligonucleotides has not been reported.

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