Kit for qualitatively detecting pagrosomus major vitellogenin, preparation method and application thereof

Abstract

The invention discloses a reagent kit for qualitatively detecting vitellogenin from Pagrosomus major and a preparation method and application thereof. During the preparation, pure vitellogenin from Pagrosomus major is prepared first; then a mouse polyclonal antibody against the vitellogenin from Pagrosomus major is prepared; and then one pure vitellogenin from Pagrosomus major, one mouse polyclonal antibody against the vitellogenin from Pagrosomus major, one PVDF membrane, one chromogenic solution, one confining liquid, one negative control group serum, and one goat anti-mouse secondary antibody labeled by horseradish peroxidase are loaded into a kit body to obtain the reagent kit for qualitatively detecting the vitellogenin from Pagrosomus major. The reagent kit can be applied to the investigation of endocrine disrupting chemicals in a marine environment, and can sensitively, accurately, conveniently and qualitatively detect the vitellogenin in blood, liver tissues, and hepatocyte culture liquid of Pagrosomus major.

Description

technical field [0001] The invention relates to a kit for qualitatively detecting red sea bream vitellogenin, a preparation method and application thereof. Background technique [0002] With the continuous improvement of the degree of industrialization in our country, the pollution of the water environment is becoming more and more serious, especially the ocean accepts the pollutants imported by the river and the industrial wastewater, domestic wastewater and aquaculture wastewater discharged from the near-shore cities. These pollutants not only destroy the living environment of marine organisms and cause serious harm to marine organisms; they can also accumulate in fish and enter the human food system, causing serious food safety problems and endangering human health. More and more reports point out that the feminization of male fish and the reduction of fish reproductive ability in marine fish are closely related to the pollution of endocrine disrupting chemicals. Polluta...

AI Technical Summary

Problems solved by technology

[0004] At present, kits for the detection of marine endocrine disrupting chemicals using marine fish vitellogenin have also been established at home and abroad, but due to the geographical restrictions on the distribution of fish and the interspecies differences of fish vitellogenin, it is not applicable in my country, and The kits used are expensive and difficult to promote