High-activity Chinese Caterpillar Fungus bacteria and oil suspension

A technology of Cordyceps sinensis fungus and oil suspension, which is applied to fungi and other directions, can solve problems such as easy contamination of miscellaneous bacteria, evaluation of the quality of Cordyceps sinensis strain preparations, and difficulty in observing the germination of single spores.

Inactive Publication Date: 2009-05-06
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the oil suspensions developed at present use spores as infecting bodies, and the quality of the preparation is evaluated by the spore germination rate, but it is difficult for Cordyceps sinensis to obtain a large number of spores through solid or liquid culture; It takes a long time to germinate, it is easy to be infected with miscellaneous bacteria, and it is difficult to observe the germination of a single spore, so it is difficult to evaluate the quality of the Cordyceps sinensis strain preparation through conventional germination experiments
At present, there are related components and contents in the literature (Research on Cordyceps sinensis strain oil suspension, Mao Xianbing, etc., China Biological Control, 24 (Supplement): 106-110, 2008) about the report of Cordyceps sinensis oil suspension However, it mainly solves the problem of improving the sedimentation volume ratio and redispersibility of the preparation. Although the cell viability has a certain relationship with the sedimentation volume ratio and redispersibility, different additives will have a significant impact on cell viability, and even Possibly significantly inhibited by the concentration of adjuvant used, experiments showed lower cell viability in this preparation

Method used

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  • High-activity Chinese Caterpillar Fungus bacteria and oil suspension
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  • High-activity Chinese Caterpillar Fungus bacteria and oil suspension

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Prepare according to the following components and contents, and prepare by stirring and mixing: measure 100 ml of solvent oil, add 5 grams of Cordyceps sinensis seed powder, 1000 mg of aluminum stearate, and 500 micrograms of surfactant (Span-80) liters; solvent oil is a mixed solution of rapeseed oil and soybean oil in any proportion; Cordyceps sinensis strain powder is obtained by drying and crushing the mycelium produced by Mortierella chinensis through liquid fermentation at low temperature.

Embodiment 2

[0056] Prepare according to the following components and contents, and prepare by stirring and mixing: measure 100 milliliters of rapeseed oil as solvent oil, add 30 grams of Cordyceps sinensis strain powder, 9000 milligrams of suspending agent (aluminum stearate), and Active agent (OP-10) 10ml; Cordyceps sinensis strain powder is a mixture of mycelium and conidia produced by Mortierella sinensis through solid fermentation.

Embodiment 3

[0058] Prepare according to the following components and contents, and make by stirring and mixing: measure 100 milliliters of solvent oil, add 10 grams of Cordyceps sinensis strain powder, 3000 mg of suspending agent (aluminum stearate), surfactant (Tween -80) 2000 microliters; wherein the solvent oil is a mixed solution of rapeseed oil and dibutyl phthalate in a volume ratio of 10:4; The fungus powder obtained by mixing the mycelia and conidia in any proportion, drying at low temperature, crushing and passing through a 120-mesh sieve.

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Abstract

The invention discloses an aweto fungal oil suspension with high energy. The aweto fungal oil suspension consists of aweto strain powder, solvent oil, a surfactant and a suspension additive. Each milliliter of the solvent oil contains 0.05 to 0.3 gram of the aweto strain powder, 5 to 100 microlitres of the surfactant and 10 to 90 milligrams of the suspension additive. A hirsutella sinensis fermentation mycelium or a mixture of a fermentation mycelium and a spore is adopted to prepare the aweto fungal oil suspension; and the strain has high energy and good stability and improves infestation rate. The aweto fungal oil suspension is suitable for the strain for artificially culturing aweto and can meet the requirement of inoculation for culturing the aweto in large scale.

Description

technical field [0001] The invention relates to the artificial cultivation of Cordyceps sinensis resources, in particular to the high-activity Cordyceps sinensis oil suspension in artificial cultivation. Background technique [0002] Cordyceps sinensis (Cordyceps sinensis) is a combination of insects and fungi formed by the fungus Hirsutella sinensis parasitizing on the larvae of bat moths. It is a unique and precious traditional Chinese medicine in my country. Due to the over-excavation of wild Cordyceps sinensis resources and the destruction of its natural growth environment, it is on the verge of extinction. my country has listed Cordyceps sinensis as a national second-class protected species. Therefore, it is of far-reaching significance to realize the artificial cultivation of Cordyceps sinensis resources. However, in all aspects of the artificial cultivation of Cordyceps sinensis, increasing the infection rate of Cordyceps sinensis inoculated with Trichospores has bec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 毛先兵刘洪波朱华李陈仕江马开森钟国跃
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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