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Tissue engineering produced tendon

A technology of tissue engineering and tendon, applied in the field of medicine and biomedical engineering, can solve the problems of infected prostheses, excretion, degradation, etc.

Inactive Publication Date: 2005-04-20
上海第二医科大学附属第九人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the biomechanical strength of the artificial tendon prosthesis is good in the short term, it will eventually degrade in the long term, and it will also cause infection and cause the prosthesis to be discharged from the body

Method used

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  • Tissue engineering produced tendon
  • Tissue engineering produced tendon
  • Tissue engineering produced tendon

Examples

Experimental program
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Effect test

preparation example Construction

[0058] The preparation method of the tissue-engineered synapse of the present invention is simple and convenient. A certain number of synaptophyte cells are mixed with a pharmaceutically acceptable biodegradable material, and then coated with a biofilm, or the synaptophyte cells are inoculated on the biofilm-coated tissue. in biodegradable materials.

[0059] The shape of the tissue-engineered tendon graft of the present invention is not particularly limited, and can be shaped arbitrarily according to the shape of tissue defect. Usually, grafts are long strips. Taking a cylinder-like graft as an example, its sides are covered with a membranous covering, and its ends may or may not be covered with a membranous covering.

[0060] The biofilm typically covers at least 60%, preferably at least 70%, more preferably at least 80%, most preferably at least 90% of the external surface area of ​​the biodegradable material.

[0061] The concentration of myosynaptic cells in the tissue-...

Embodiment 1

[0070] Isolation and expansion of tenocytes in vitro

[0071] Seeding cells is an essential element in tissue engineering research. The application of tissue engineering technology to construct tendon tissue requires the expansion and culture of as few cells as possible in vitro to reach the number of cells required for the construction of tendon tissue. In this example, the enzymatic digestion technique was used to separate the tenocytes in the tendon tissue, and expanded and cultured them in vitro.

[0072] (1) Isolation of Tenocytes

[0073] Cut out II-IV toe Leghorn deep flexor tendon (tendon sheath area) under aseptic condition, and cut the tendon tissue into 1×2×2mm 3 Put the tissue block into a centrifuge tube and wash it three times with PBS, add 0.25% type II collagenase, place it on a shaking table at 37°C for digestion, and collect tenocytes at 4, 5, and 7 hours respectively. Obtain isolated myocytes by harvesting as follows:

[0074] 1. Pass the digestive juice...

Embodiment 2

[0094] Fabrication of Biomaterial Scaffolds and Formation of "Cell-Biomaterial" Complexes

[0095] Another key factor in tissue engineering technology is the biomaterial scaffold. Cells grow on the biomaterial scaffold and secrete extracellular matrix. In the early stage, the biomaterial scaffold plays the role of extracellular matrix. As time goes by, the cells secrete extracellular matrix gradually increase, and the biomaterial scaffold gradually degrades. In this dynamic process, the optimal way is to achieve a dynamic balance between the production of extracellular matrix and the degradation of biomaterial scaffolds. Biomaterials also need to have good biocompatibility and porosity (after cells adhere to the biomaterial, they can not only contact each other, but also facilitate the exchange of nutrients and the discharge of metabolic waste), good biomechanical properties, and can Replace the biomechanical function of the tissue to be repaired. In this example, polyglycol...

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Abstract

The present invention provides a tissue engineered tendon graft, which comprises: (a) a pharmaceutically acceptable biodegradable material; (b) tenocytes, the tenocytes are inoculated on the biodegradable material; (c) biofilm, said biofilm coating the outside of the biodegradable material. The tissue engineered tendon of the present invention can effectively treat tendon defect. The invention also provides the preparation method and application of the tissue engineered tendon.

Description

technical field [0001] The present invention relates to the fields of medicine and biomedical engineering, and more specifically relates to tissue-engineered tendon and its preparation method and application. Background technique [0002] Tendon is a bundle of dense collagen fibrous connective tissue that connects the belly of skeletal muscle to bone, and is an integral part of skeletal muscle. The structure of the tendon can be regarded as the continuation and degeneration of the muscle. The tendons of fresh specimens are silvery-white, cord-like, and tough in texture. There are ripples on the surface in the relaxed state. If the tension exceeds 4% of the relaxed state, the ripples will disappear, and the ripples will reappear after the tension is removed. Tendons and muscles differ markedly in morphology, structure, and function. Muscle is a highly specialized tissue with contractility, rich blood supply, strong metabolism, and strong anti-infection ability; while tendo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/02A61L27/00
Inventor 曹谊林
Owner 上海第二医科大学附属第九人民医院
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