Method for promoting growth of Habrotrocha sp. using bee honey residue

A technology of rotifers and residues, applied in the field of bioactive substances to promote the growth of rotifers, can solve the problems of high economic cost of sewage treatment, increase the lifespan and fertility of rotifers, achieve a significant growth-promoting effect, realize recycling, The effect of growth promotion

Active Publication Date: 2009-05-20
NANTONG KANGDE BIOLOGICAL PROD
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Problems solved by technology

The current study found only a few tocopherol compounds that increase the lifespan and

Method used

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Example Embodiment

[0016] The embodiments of the present invention are:

[0017] (1) Prepare the culture solution of rotifers with a ratio of 0.06~1.00g C a CO 3 , 6~8mL 1M phosphate buffer (pH=6.5), add about 1000mL of distilled water, boil and sterilize;

[0018] (2) Use broth medium to cultivate staphylococci in large quantities, and use it as rotifer food after centrifugation;

[0019] (3) Pre-cultivation: Collect rotifers from the aerobic process wastewater of Shanghai Quyang Wastewater Treatment Plant, place them in a petri dish with an inlet diameter of 60mm after separation, and add 8 mL of the culture solution of step (1) and step (2) Staphylococcus 10 6 ~10 8 cells·mL -1 , Honey residue 5μg, cultured in a 25℃ light incubator in the laboratory, the pre-cultivation time lasts for 7 days;

[0020] (4) Put 6 rotifer larvae randomly picked from the pre-cultured petri dish (without oval rotifers) into each petri dish, add 8 mL of rotifer culture solution, 10 staphylococci 6 ~10 8 cells·mL -1 , T...

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Abstract

The invention discloses a method for promoting the growth of Habrotrocha sp by using honey residue, which belongs to the technical field of water pollution control. The aim of effectively promoting the growth of the Habrotrocha sp is achieved by adding the honey residue rich in active substances such as flavonoid, terpenes and so on. The method comprises the following steps: the honey residue is added into a Habrotrocha sp culture fluid prepared by CaCO3, a phosphate buffer solution and distilled water, and staphylococcus is taken as food of the Habrotrocha sp to cultivate the Habrotrocha sp in an illumination cultivating box with the temperature of 25 DEG C. The method selects food production waste, namely the honey residue as an additive to promote the growth of the Habrotrocha sp, which has remarkable growth promotion effect on the Habrotrocha sp, can ensure that the population growth rate reaches 0.87d<-1> in an increased logarithmic phase of the Habrotrocha sp, and is far higher than 0.55d<-1> when the honey residue is not added on one hand, can ensure that the honey residue is turned into valuables, realize the cyclic utilization of resources, and also ensure that the method is more economical when practically applied to sewage treatment in the future on the other hand.

Description

technical field [0001] The invention belongs to the technical field of water pollution control, in particular to a method for promoting the growth of Habrotrocha sp. with biologically active substances. The purpose of effectively promoting the growth of rotifers is achieved by adding honey residue rich in active substances. Background technique [0002] Rotifer, a small metazoan that lives in clumps on substrates (activated sludge, biofilms) and feeds on suspended particles, contributes greatly to the reduction of suspended solids in sewage treatment systems and the enhancement of perspective, However, its density in sewage treatment plants is not high. Therefore, it is of great significance to try to promote the growth of rotifers and increase their density for sewage treatment. [0003] Biologically active substances are a type of plant or animal endogenous substances that have specific active functions on biological organisms. At present, biologically active substances ...

Claims

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Application Information

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IPC IPC(8): A01K67/033A23K1/18C02F3/32A23K50/90
Inventor 丁国际杨芳芳沈成媛
Owner NANTONG KANGDE BIOLOGICAL PROD
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