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Preparation and use of Linaria vulgaris flavone and total flavone thereof

A technology of willow fish and total flavonoids, which is applied in the field of medicine to achieve the effect of simple extraction method, environmental protection and increased content

Inactive Publication Date: 2013-08-28
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The medicinal material of willow fish mainly contains flavonoids, and there is no report about the preparation method of willow fish flavonoids and total flavonoids and their use in the preparation of drugs for treating cardiovascular diseases, relieving cough and asthma

Method used

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  • Preparation and use of Linaria vulgaris flavone and total flavone thereof
  • Preparation and use of Linaria vulgaris flavone and total flavone thereof
  • Preparation and use of Linaria vulgaris flavone and total flavone thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, the preparation of total flavonoids of willow piercing fish

[0028] Dried the harvested willow chrysalis and cut into sections. Add 10 times the crude drug amount of 90% ethanol to the obtained willow chutney and reflux extract it for 3 times, each extraction time is 1.5h, collect the extract, filter, The obtained filtrate was concentrated and dried to obtain the extract of Liu Chuanyu, and the obtained extract was loaded on HPD-600 type macroporous resin with 0.5g / ml, and the sample loading flow rate was 3BV / h, and the column diameter-to-height ratio was 1: 5. Elute with 90% ethanol solution. The eluate is filtered, and the filtrates are combined, concentrated, dried, and pulverized to obtain the total flavonoids of the willow fish. The total flavonoids of the chrysalis of the present invention is a yellow-brown powder, and the absorptivity is measured at 280nm by spectrophotometry, and the content of the total flavonoids is calculated to be 66.8%.

Embodiment 2

[0029] Embodiment 2, the preparation of total flavonoids of willow piercing fish

[0030] Dried the harvested willow chrysalis and cut into sections, adding 8 times the crude drug amount of 80% ethanol to the obtained willow chutney crude drug for reflux extraction twice, each extraction time was 1 hour, collected the extract, filtered, and obtained The filtrate was concentrated and dried to obtain the extract of Liuchuanyu, and the obtained extract was loaded on HPD-600 type macroporous resin with 0.4g / ml, and the sample loading flow rate was 3BV / h, and the column diameter-to-height ratio was 1:3. , and eluted with 90% ethanol solution. The eluate is filtered, and the filtrates are combined, concentrated, dried, and pulverized to obtain the total flavonoids of the willow fish. The total flavonoids of the chrysalis of the present invention is a yellow-brown powder, and the absorptivity is measured at 280nm by spectrophotometry, and the content of the total flavonoids is calcu...

Embodiment 3

[0031] Embodiment 3, the preparation of the total flavonoids of willow piercing fish

[0032] Dried the harvested willow chrysalis and cut into sections. Add 12 times the crude drug amount of 70% ethanol to the obtained willow chutney and reflux extract it for 3 times, each extraction time is 0.5h, collect the extract, filter, The obtained filtrate was concentrated and dried to obtain the extract of Liu Chuanyu, and the obtained extract was loaded on HPD-600 type macroporous resin with 0.4g / ml, and the sample loading flow rate was 3BV / h, and the column diameter to height ratio was 1: 3. Elute with 90% ethanol solution. The eluate is filtered, and the filtrates are combined, concentrated, dried, and pulverized to obtain the total flavonoids of the willow fish. The total flavonoids of the chrysalis of the present invention is a yellow-brown powder, and the absorptivity is measured at 280nm by spectrophotometry, and the content of the total flavonoids is calculated to be 60.8%. ...

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Abstract

The invention belongs to the technical field of medicine, and relates to a method for preparing flavonoids and total flavonoids in Linaria vulgaris and application thereof. The flavonoids and the total flavonoids come from flavonoid compounds in the Linaria vulgaris (including flavonoid compounds such as pectolinarin, aglycon, acetyl pectolinarin, acetyl linarin, acetyl linarin, hesperidin, robinin, hispidulin, luteolin, vanilla lignin, chrysin and the like). Dried whole herbs of the Linaria vulgaris are subjected to reflux extraction by water or alcohol, then a macroporous resin is used to send to a column, alcohols with different concentrations are used for elution, eluents are collected, then different elution flow ingredients pass through a silica gel chromatographic column, a polyamide chromatographic column, a gel chromatographic column and the like, to obtain various compositions, and the content of an obtained total flavonoid substance is more than 60 percent. The flavonoid compounds can be used for preparing medicines for treating angiocardiopathy, cough, and asthma. An extraction method of the invention is simple, practical and cheap, and is suitable for industrialized production.

Description

technical field [0001] The present invention relates to the field of medical technology, and relates to a preparation method and application of flavonoids of scalloped fish and its total flavonoids, in particular to a preparation method of total flavonoids and various flavonoids derived from the medicinal material of scalloped fish and its preparation method. Use in medicines for treating cardiovascular disease, cough and asthma. Background technique [0002] Linaria vulgaris ssp. sinensis (Bebeaux) Hong is the dried whole herb of Linaria vulgaris ssp. It is native to the temperate zone in northern Eurasia, in sandy land, hillside grassland and roadside. Light-loving, more cold-resistant, not tolerant to extreme heat, should be moderately fertile, moderately moist and well-drained soil. [0003] Foreign folks use the plants of the genus Salix as diuretics, laxatives, antispasmodics, choleretics and anti-inflammatory drugs for the treatment of cystitis, bleeding, rashes, et...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07D311/30C07D311/40A61K31/7048A61K31/352A61K36/80A61P9/00A61P11/14A61P11/06
Inventor 刘晓秋华会明关宏峰兰洲
Owner SHENYANG PHARMA UNIVERSITY
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