90 results about "Hesperidina" patented technology

The invention discloses a method for preparing neohesperidin by an enzymic method, comprising the following steps: performing coordination between hesperidin and metal ions and dissolving in water, and realizing conversion from hesperidin to neohesperidin under the action of immobilized rhamnosidase and immobilized rhamnoside transferase. The method provided by the invention is adopted to solve the problem that production cost of neohesperidin widely used in medical, functional food and its cosmetic fields is high, and simultaneously opens up a new approach for recycling of industrial waste orange peel and high value-added utilization of hesperidin.

The invention relates to the technical field of plant extraction and provides a method for extracting hesperidin from fructus aurantii immaturus according to a continuous countercurrent method and theextracted hesperidin. The method for extracting hesperidin from fructus aurantii immaturus includes: providing fructus aurantii immaturus infiltrated by alkaline alcoholic solution, mixing with alkaline alcoholic solution, performing continuous countercurrent extraction, and crystallizing extract liquid obtained in continuous countercurrent extraction to obtain hesperidin. The method is high in extraction efficiency, extraction time can be effectively shortened, and accordingly productivity is improved; in addition, extraction solvent quantity can be effectively decreased, and accordingly alcohol consumption and alcohol recovery energy consumption are sharply reduced, and cost reduction is realized; moreover, the extract liquid is high in product concentration, concentration difference ofextract liquid in different time periods is avoided, and stable product quality is realized after crystallization. The hesperidin is obtained by application of the method for extracting hesperidin from fructus aurantii immaturus. The hesperidin has advantages of high purity and quality stability and is high in market value and worthy of popularization and application.

The present invention provides a multiple index component and fingerprint determination method of a liver-tonifying eyesight-improving oral liquid, and the method aims at chlorogenic acid, luteoloside, ferulic acid, nobiletin, paeoniflorin and hesperidin. The determination method is simple, strongly-specific, good in stability, high in precision, good in reproducibility and high in sample recovery rate. Further, the present invention also provides a preparation method of the liver-tonifying eyesight-improving oral liquid and the liver-tonifying eyesight-improving oral liquid prepared by the preparation method, and the preparation method comprises the step of the determination method.

The invention relates to a semi-synthesis method of luteolin and galuteolin as well as luteolin rutinoside by using hesperidin, a semi-synthesis method of the galuteolin by using hesperetin glucoside, and a semi-synthesis method of the luteolin by using hesperetin, and belongs to the field of chemistry and medicines. The semi-synthesis method comprises the following steps: enabling the hesperidin, the hesperetin glucoside and the hesperetin to be subjected to complexation in pyridine alcohol fluid, dehydrogenizing by using iodine, directly distilling alcohol and pyridine, maintaining for a period of time in an airtight distilled state, and carrying out demethylation reaction, so that the hesperidin is generated into the luteolin rutinoside; generating the galuteolin by demethylation of diosmetin glucoside, and generating the luteolin by diosmetin; and hydrolyzing the luteolin rutinoside, so that the luteolin rutinoside is transformed into luteolin and galuteolin. The semi-synthesis method has the advantages that two steps of dehydrogenation and demethylation are combined into one step, and reaction conditions of dehydrogenation and demethylation are mild and easy to control; few reagents are used and green and environmentally friendly; and the demethylation yield is high, and the industrial production is easy. Compared with disclosed documents and patients, the semi-synthesis method has great advantages in the production of luteolin and glucosides of the luteolin.

The invention provides a continuous flow microchannel synthesis process of flavonoid compounds. According to the process, hesperidin and iodine elementary substance are used as raw materials and react in a continuous flow microchannel reactor in the presence of a reaction solvent to synthesize the flavonoid compound as shown in a formula A. Compared with a traditional kettle-type preparation process, the process disclosed by the invention has the advantages that the preparation time is obviously shortened, and the conversion rate of raw materials and the yield of products are obviously improved; and especially, when the diosmin is prepared under optimal process conditions of continuous flow microchannel synthesis, the conversion rate of the raw material hesperidin is as high as 96.48%, and the yield of the product diosmin is as high as 81.96%. The continuous flow micro-channel synthesis process provided by the invention is beneficial to realizing safe, efficient and rapid industrial production of flavonoid compounds, and has a wide application prospect.

The invention relates to a quality control method for a liver-soothing qi-regulating pill as traditional Chinese preparation, which is prepared from traditional Chinese herbal medicines as raw materials, belonging to the technical field of Chinese patent medicine; the method comprises the steps that: with magnolol, honokiol and cyperone as reference substances, thin-layer chromatography is adopted to identify whether the formula of the liver-soothing qi-regulating pill contains the ingredients of magnolol, honokiol and cyperone; and with hesperidin as a reference substance, high efficiency liquid chromatography is adopted to detect the content of the hesperidin in the formula of the liver-soothing qi-regulating pill. The revised quality standard is more perfect and the quality control for medicines is improved.

The invention relates to a clean preparation process of high-purity hesperetin. The process mainly comprises the following steps: (1) hydrolyzing hesperidin to obtain the hesperetin, (2) concentrating hesperetin reaction liquid in a vacuum mode, (3) depositing the hesperetin concentrated solutions, (4) preparing the hesperetin, and (5) processing filter pressing acid water solutions. According to the process, hesperetin which is more than 95% in content (high performance liquid chromatography (HPLC)) is obtained through a further reaction and a special processing process. Compared with the facts that in prior art, a crude product is firstly obtained through hydrolyzing, and then a fine product is obtained by refining the crude product, the clean preparation process of high-purity hesperetin has the advantages of being simple in process, low in cost, and high in yield. Acid water which is generated by hydrolyzing is well processed and recycled, rhamnose in the acid water is abstracted, and crystallization mother liquor is prepared into agricultural nucleic acid. Therefore, the whole production process is resource-saving and environment-friendly.

The invention relates to the field of natural product preparation, and relates to a comprehensive utilization method of immature bitter oranges. According to the method, the step of enzymolysis pretreatment is added, so that the hesperidin extraction efficiency is improved, the extraction time is shortened, and the amount of an extraction solvent is reduced, the damage to functional components inthe long-time extraction process is avoided, and the comprehensive utilization of immature bitter oranges can be realized. The comprehensive utilization method can be applied to production practice ofextraction of hesperidin, the extraction rate and purity of hesperidin are improved, and the comprehensive utilization rate of the raw material immature bitter oranges is further guaranteed.

A carbonated beverage comprises the following raw materials per 1000mL: 2-3g citric acid, 0.2-0.8g sodium citrate, 5-10g fructo-oligosaccharide, 8-12mg neohesperidin dihydrochalcone and 0.2-1g pigment. A carbon dioxide content is not less than 3%. The carbonated beverage is prepared by water treatment, chilled water treatment, batching, premixing, filling, sealing, code spraying, light inspection, temperature return, labeling, water removal, inspection, warehousing and the like. In the carbonated beverage, fructo-oligosaccharide and neohesperidin dihydrochalcone serve as sweetening agents, so that a synergistic effect is enhanced, and a taste is mediated. The carbonated beverage is simple in process and good in taste, has multiple health-care effects at the same time, is an ideal fashionable drink, and is suitable for various human bodies with diabetes and adiposity.

The invention discloses a low-cost preparation method of diosmin. The low-cost preparation method of diosmin comprises the following steps: dissolving hesperidin and iodine in pyridine; performing heating and introducing ozone until the concentration of hesperidin is lower than 5%; adjusting the pH value to be neutral, and performing filtering to obtain a first filtered substance; washing the first filtered substance with deionized water, adding alkali for dissolving, and performing filtering to obtain filtrate; and acidizing and recrystallizing the filtrate to obtain diosmin. The method has the effects of reducing the dosage of iodine and lowering the cost.

The invention discloses an extraction method of an immature bitter orange extract. The extract comprises pectin, synephrine, hesperidin, total flavonoids of immature bitter oranges, plant fibers and the like. The extraction method comprises the steps: selecting an immature bitter orange raw material with a diameter of less than 1.5 cm; extracting with a hydrochloric acid solution to obtain a mixture of pectin and synephrine, and further separating and purifying to obtain pectin and synephrine products; extracting with a sodium carbonate solution to obtain an immature bitter orange total flavone product; extracting with sodium hydroxide to obtain a hesperidin product; and the finally extracted residues are pure plant fibers. Various products are comprehensively developed from immature bitter oranges by using various methods, and the complete utilization of materials is ensured so that the production cost is reduced, and the environmental pollution is reduced.

The invention discloses a steaming processing method of dried tangerine or orange peel decoction slices. The steaming processing method comprises the following steps: step 1, performing cleaning; to be specific, removing residual fruit stems, leaves and non-medicinal substances; step 2, performing medicine washing; to be specific, pouring the medicinal materials into a stainless steel medicine washing pool or medicine washing machine, washing the medicinal materials with tap water to mainly remove surface impurities such as dust, washing the medicinal materials with water for about 2-3 minutes, and fishing out the medicinal materials with a leaking net; step 3, performing steaming; to be specific, putting the cleaned dried tangerine or orange peel medicinal materials into a medicine steaming machine, performing steaming for 30-120min, and performing moistening for 0-12h; and step 4, performing cutting; to be specific, cutting the steamed dried tangerine or orange peel medicinal materials and the like. According to the method, the dried tangerine or orange peel steaming technology is optimized through multiple indexes such as the hesperidin amount and the characteristic spectrum ofsteamed dried tangerine or orange peel is established; and the manufacturing process is quantified.

The invention discloses a method for separating synephrine from hesperidin waste liquid, which comprises the following steps: S1, loading a hesperidin mother liquid to strongly acidic cationic resin, after loading is finished, eluting with water, after water elution is finished, eluting with a strong alkali mixed liquid, collecting an eluate after elution with the strong alkali mixed liquid, and adjusting the pH value of the eluent to 5-7 to obtain a neutralized solution; and S2, carrying out ultrafiltration on the neutralized solution, then carrying out concentration through a nanofiltration membrane, and carrying out cooling crystallization, filtration and drying on the concentrated solution to obtain synephrine. According to the method for separating synephrine from the hesperidin waste liquid, the hesperidin waste liquid is reused, novel poncirin is extracted from the hesperidin waste liquid, the problem of sewage treatment is solved while economic benefits are obtained, and waste recycling is achieved. According to the method, ion exchange is carried out through the strongly acidic cationic resin, elution is carried out through the low-concentration strong alkali mixed solution, no strong pungent smell exists, the requirements for production equipment and the environment are low, and the requirement for green production is met.

The invention belongs to the technical field of traditional Chinese medicines, and specifically relates to application of a boenninghausenia sessilicarpa cough-asthma tablet to prepare medicaments for inhibiting lung cancer cell LLC proliferation and a preparation method for the boenninghausenia sessilicarpa cough-asthma tablet. The boenninghausenia sessilicarpa cough-asthma tablet provided by the invention possesses the application to prepare medicaments for inhibiting lung cancer cell LLC proliferation. The boenninghausenia sessilicarpa cough-asthma tablet is prepared from the following bulk drugs: 156 g of dried orange peel, 96 g of acorus gramineus, 312 g of reynoutria japonica, 188 g of radix asparagi, 375 g of boenninghausenia sessilicarpa, 96 g of stemona sessilifolia, 750 g of caulis marsdeniae tenacissimae, 375 g of laggera pterodonta, 96 g of semen armeniacae Amarum., 375 g of houttuynia cordata and 188 g of cortex mori. Supercritical extraction and microwave extraction are employed for preparing the boenninghausenia sessilicarpa cough-asthma tablet, and thus hesperidin content is substantially improved.

The invention belongs to the field of pharmaceutical preparations, and discloses a method for improving the water solubility and bioavailability of hesperidin. A cyclodextrin embedding method is adopted, an alkalic system is used for solubilization, and the hesperidin is embedded in an inner cavity of cyclodextrin to prepare a hesperidin-cyclodextrin embedded object, so that the water solubility of the hesperidin is obviously improved, meanwhile the side reaction of hesperidin ring opening caused by the conventional technical means is avoided, the structural integrity of the hesperidin is ensured, and the oral bioavailability of the hesperidin is remarkably improved.

The invention discloses a method for preparing natural hesperidin through biological fermentation and extraction. Firstly, a fermentation culture solution is prepared, and is mixed with fresh tangerine peel or orange peel; then steam explosion treatment is performed, the material is inoculated with microorganisms and culture is performed, then parameters such as temperature and ventilatory capacity are adjusted for enzymolysis, crystallizing is performed after the enzymolysis is completed, and filtering is performed to obtain the natural hesperidin. The yield of the natural hesperidin can reach 42%. According to the method, the fresh tangerine peel or orange peel is directly used as the raw material, and operations of drying, crushing and the like are not needed, so that degradation of the hesperidin under the high-temperature condition in an extraction stage is avoided, the pollution of alkali liquor such as lime water to the environment is also avoided, the equipment requirement in the whole process is simple, the process flow is short, and the extraction yield of the hesperidin is high.

The invention provides a process for extracting hesperidin and tangerine peel yellow pigment from orange peel simultaneously. The process comprises the following steps: performing reflux extraction on dried orange peel with acid alcohol, filtering, concentrating, neutralizing, further filtering, adding alkali into the filtering residues to dissolve, neutralizing, and crystallizing, thereby preparing a pure hesperidin product; and adding ethanol into the filtrate to extract, and concentrating and drying, thereby preparing a pure tangerine peel yellow pigment product. The process is simple to operate, the production costs of tangerine peel yellow pigment and hesperidin are greatly lowered, and the production process is simplified.

The invention discloses a method for green preparation of silver nanoparticles through synergy of hesperidin and pectin and application. The method comprises the specific steps that (1) a silver nitrate solution and a sodium hydroxide solution are prepared; (2) accurately weighing a hesperidin and pectin mixture according to a ratio of 3:1-1:3, adding a sodium hydroxide solution, placing in a conical flask to form a hesperidin-pectin alkali solution, and performing auxiliary dissolution in a microwave oven; (3) adding a silver nitrate solution into the solution, uniformly shaking, putting into the microwave oven, carrying out microwave-assisted reaction, and standing; and (4) preferentially taking reaction conditions according to an ultraviolet absorption peak value of about 400nm, taking the reacted liquid for high-speed centrifugation, taking the precipitate, adding a proper amount of distilled water, repeatedly centrifuging twice, and drying the precipitate in a drying oven overnight to obtain the silver nanoparticles. The preparation process is simple, the cost is low, the prepared product has the biological characteristics of nano-silver, such as broad-spectrum antibacterial activity, and also has the common biological activity of the hesperidin and the pectin.