31 results about "Hesperidina" patented technology

The invention relates to a beauty and skin care enzyme and a preparation method thereof. According to the invention, fruits and traditional Chinese medicines are used as main fermentation raw materials, the fruits and the traditional Chinese medicines are crushed and mixed with fermentation strains, and active substances in each raw material are effectively retained through two-stage fermentation, filtration separation and freeze-drying treatment, SOD and sea buckthorn flavone contained in sea buckthorn fruits have the skin care effects of resisting oxidation and promoting collagen synthesis, and the sea buckthorn fruits have the skin care effects of resisting oxidation and promoting collagen synthesis. Salidroside contained in the rhodiola rosea has the skin care effects of resisting light aging and fatigue, astragalus polysaccharide and astragaloside contained in the astragalus membranaceus can repair a barrier and enhance immunity, ophiopogonin and polysaccharide contained in the radix ophiopogonis have the effects of preserving moisture and resisting saccharification, and hesperidin and nobiletin contained in the pericarpium citri reticulatae have the effects of resisting oxidation and adjusting grease secretion. The obtained compound enzyme has the effects of resisting oxidation, resisting inflammation and preserving moisture, has activity, stability and transdermal efficiency, and can be used in skin care products such as anti-oxidation essence, repair masks, late-up cream and the like.

The invention discloses a composition and application thereof, and the composition comprises synephrine or a pharmaceutically acceptable salt thereof, and further comprises naringin, neohesperidin and / or hesperidin. The composition prepared by compounding the synephrine or the pharmaceutically acceptable salt thereof with the naringin, the neohesperidin and / or the hesperidin has the effects of relieving, repairing, resisting wrinkles, easing pain, relieving itching or resisting allergy when being used as a functional component of a skin care product; when being used as a functional component of a scalp care product, the shampoo has the effects of removing dandruff, removing oil, relieving itching or preventing alopecia.

Compositions that contain flavonoids such as hesperidin, quercetin, hesperetin, and rutin and methods of treating viral diseases such as coronavirus (e.g., SARS COV-2) infection, influenza virus infection, rhinovirus infection, and human metapneumovirus infection.

The bioplastic additives and antimicrobial plastics include polyphenols and organic polymers. The polyphenol can be a phenol, a polyphenol, a bioflavonoid, a flavonoid, a tannin, a coumarin, a lagman, a quinone, a stilbene, or a qurcuminoidenin. The organic polymer may be chitosan, chitin, cellulose, and keratin. In an advantageous form, the polyphenol is hesperidin, and the organic biopolymer is chitosan.

The application provides a method for detecting the pharmacodynamic components of a traditional Chinese medicine composition by UPLC-MS / MS. The method can identify 14 pharmacodynamic components of the traditional Chinese medicine composition, including saikosaponin A, saikosaponin D, naringin, paeoniflorin, hesperidin, chicoric acid, chlorogenic acid, salvianolic acid B, zephyranthine, kaempferitrin, and codariusrhizome glycoside, amargin, hyperoside and rutin, thereby providing a reference for evaluating the intrinsic quality of the traditional Chinese medicine composition.

The present application relates to the technical field of food packaging preservation material preparation, and particularly relates to a carbon quantum dot-polyvinyl alcohol composite film and a preparation method and application thereof. The carbon quantum dot-polyvinyl alcohol composite film comprises polyvinyl alcohol, a plasticizer and hesperidin carbon quantum dots. The raw materials used in the present application are degradable or easy to recycle, which significantly reduces environmental pollution. By converting waste agricultural and sideline resources into valuable carbon dots, it has important value for realizing the high value of agricultural and sideline resources. The present application studies the role of hesperidin carbon quantum dots in preservation, which has important significance for developing new preservation technology, improving food storage life and preservation quality.

The invention discloses saccharomyces cerevisiae and a method for efficiently synthesizing neohesperidin by using cane sugar. According to the saccharomyces cerevisiae, on the basis of ZQH01, FjTAL derived from Flavobacterium johnsoniae, AtC4H, AtPAL2 and AtATR2 derived from Arabidopsis thaliana and CYB5 derived from yeast are inserted into a genome of the ZQH01, and the saccharomyces cerevisiae is prepared by the following steps of: adding the FjTAL, the AtC4H, the AtPAL2 and the AtATR2 into the genome of the ZQH01; according to the invention, a sucrose phospholysis double-carbon-source system is introduced into the saccharomyces cerevisiae, sucrose can be directly converted into fructose and glucose-1-phosphoric acid, the fructose is specially used for cell growth and hesperetin synthesis, the glucose-1-phosphoric acid directionally supplies UDP-glucose, and a carbon flow is physically separated to eliminate flux competition caused by a Crab effect. The saccharomyces cerevisiae disclosed by the invention can be used for efficiently synthesizing neohesperidin by using cane sugar, and has important significance on green and efficient acquisition of citrus flavonoids.

The objective of this invention is to provide a beverage containing dead lactic acid bacteria, in which the off-flavor of the culture medium for lactic acid bacteria is suppressed, and a method for manufacturing the same. This objective is achieved when the concentration of dead lactic acid bacteria (one or more) at 1.3 billion cells / L or higher, and the concentration of hesperidin at 10–1000 mg / L.

This invention discloses a method for extracting hesperidin, belonging to the field of natural product extraction technology. The method includes the following steps: S1, pulverizing and sieving the raw material to obtain raw material powder; mixing the raw material powder with an extractant and performing ultrasonic-assisted extraction to obtain an extract; concentrating the extract to obtain crude hesperidin; S2, adding ethanol solution to the crude hesperidin extract, allowing it to stand, and filtering to obtain an alcohol extract; S3, adjusting the pH of the alcohol extract to acidic using acid, stirring, filtering, and collecting the precipitate; S4, dissolving the precipitate with an alkaline solution, filtering, adjusting the pH of the filtrate to acidic using acid, precipitating the precipitate, and drying to obtain crude hesperidin; S5, dissolving the crude hesperidin in ethanol solution, purifying it using macroporous resin, and freeze-drying to obtain pure hesperidin. This extraction method maintains the structural integrity of hesperidin and achieves high extraction rate and high purity.

The present invention belongs to the technical field of pharmaceutical formulations. Specifically, provided is a method for preparing a traditional Chinese medicine nano-micelle formulation encapsulating hesperidin. The specific steps and process of the method for preparing the traditional Chinese medicine nano-micelle formulation encapsulating hesperidin are as follows: a polyethylene glycol-polylactic acid block copolymer, hesperidin, dichloromethane and poloxamer 188 are selected for later use. According to the present application, the traditional Chinese medicine nano-micelle formulation encapsulating hesperidin is prepared by means of mixing the polyethylene glycol-polylactic acid block copolymer, hesperidin, dichloromethane and poloxamer 188. The preparation process is simple and involves single raw material selection, such that the production cost is effectively controlled. The preparation method is suitable for industrial large-scale production and can be widely used in clinical practice.

The application belongs to the technical field of content detection, and discloses a kind of HPLC detection methods for simultaneously determining the content of multiple components in semen Cannabis capsules.The detection method includes mixing reference solution, chrysophanol and methanol to obtain mixed reference solution.The reference solution includes physcion solution, magnolol solution, houpo chrysophanol solution, emodin solution, rhein solution, nobiletin solution, aloe emodin solution, hesperidin solution and paeoniflorin solution;The contents of semen Cannabis capsules are mixed with methanol, and the test solution is obtained after heating and refluxing;The mixed reference solution and test solution are respectively sucked and injected into high performance liquid chromatograph.The detection method can simultaneously determine the content of multiple index components, can comprehensively reflect the quality of the preparation, and the test method has excellent precision, repeatability, stability and high sample recovery rate.

The present application relates to a kind of traditional Chinese medicine composition containing heat-sensitive component and its preparation method and application, the preparation method of the traditional Chinese medicine composition containing heat-sensitive component of the present application does not use a large amount of anhydrous ethanol for alcohol precipitation, avoid the loss of polysaccharide active ingredient precipitation, also does not use high temperature extraction exceeding 80 DEG C, avoid the loss of heat-sensitive component such as hawthorn acid and hesperidin.The preparation method of the present application combines enzyme engineering, countercurrent kinetics, supercritical fluid and other technologies, while ensuring the extraction efficiency, reduce the loss of heat-sensitive component such as hawthorn acid and hesperidin and sugar active ingredient, improve extraction efficiency and reduce energy consumption cost, and the amount of anhydrous ethanol is reduced by more than 70%, in line with GMP clean production standard.

The invention belongs to the field of traditional Chinese medicine analysis, and particularly relates to a method for determining the content of Zhuanshu granules by combining a specific chromatogram with multiple indexes. According to the method, a high performance liquid chromatography method is adopted, acetonitrile is taken as a mobile phase A, a 0.05-0.2% phosphoric acid aqueous solution is taken as a mobile phase B, the gradient elution condition is 0 min, the mobile phase A accounts for 14-16%, and the mobile phase B accounts for 84-86%; the mobile phase A accounts for 18-22%, and the mobile phase B accounts for 78-82%; the mobile phase A accounts for 38-42% and the mobile phase B accounts for 58-62% in 25 min, and the mobile phase A accounts for 38-42% and the mobile phase B accounts for 58-62% in 40 min. The specific chromatogram of 13 chromatographic peaks with hesperidin as a reference substance is established for the first time, qualitative and quantitative evaluation of multiple medicine flavors in the prescription is achieved at the same time, operation is easy and convenient, the cost is reduced, the efficiency is improved, and the overall quality control level of the Zhuanshu granules is improved.

This invention relates to a complex method for improving the solubility of hesperidin in concentrated orange juice, belonging to the field of food processing technology. Specifically, it relates to a technique that combines specific functional components and a physical field to efficiently and stably improve the solubility of hesperidin in concentrated orange juice. The main steps of this invention include: raw material selection, preparation of concentrated orange juice, preparation of a functional polysaccharide-EGCG composite cosolvent, addition of the functional polysaccharide-EGCG composite cosolvent, ultrasonic treatment, sterilization, static magnetic field-assisted cooling, and filling. The functional polysaccharide-EGCG composite cosolvent is synthesized by the self-assembly of functional polysaccharides and epigallocatechin gallate (EGCG); the static magnetic field-assisted cooling involves applying a low-temperature static magnetic field to the concentrated orange juice after pasteurization. Compared with concentrated orange juice prepared by untreated or single-treatment methods, this invention can significantly improve the solubility of hesperidin in concentrated orange juice, contributing to the quality improvement of such products.

The application discloses a method for preparing pet food attractant with the flavor of stink mandarin fish by using mandarin fish by-products, and comprises the following steps: subjecting stink mandarin fish fermentation liquor to microfiltration concentration-in-situ embedding treatment to obtain high-abundance liquid microsphere leavening agent; subjecting stink mandarin fish by-product raw materials to step-by-step enzymatic hydrolysis process treatment to obtain high-hydrolysis-degree fermentation substrate; mixing the fermentation substrate with the liquid microsphere leavening agent to perform anaerobic fermentation, and then removing bacteria by microfiltration to obtain liquid food attractant with the flavor of stink mandarin fish; using hesperidin / xanthan gum / corn starch to prepare microcapsules for encapsulating the liquid food attractant, and obtaining pet food attractant with the flavor of stink mandarin fish after freeze-drying. The method is green and environmentally friendly throughout, improves the utilization rate and bioavailability of mandarin fish by-products, realizes the customized production of the characteristic flavor of stink mandarin fish, and realizes the long-period stable release of flavor compounds, and the introduction of hesperidin through embedding effectively enhances the functional activity of the product, and meets the diversified needs of pet food at present.

The invention provides a separation method of a neohesperidin finished product, and belongs to the technical field of separation and purification. In the implementation process, a whole set of separation treatment including alkali dissolution, membrane separation, acid precipitation, concentration, cooling crystallization and centrifugal separation is carried out on the neohesperidin obtained after fermentation is finished, the yield of the obtained neohesperidin finished product is about 85%, and industrialization of the neohesperidin fermentation process is achieved.

A fructus cannabis intestine-moistening pill high performance liquid chromatography fingerprint establishment method relates to the field of medicine, and comprises the following steps: weighing fructus cannabis intestine-moistening pill medicinal material powder, adding methanol, plugging, weighing, soaking, carrying out ultrasonic extraction, cooling, weighing, complementing the weight loss, uniformly shaking, and filtering to obtain a test solution; the method comprises the following steps: weighing an emodin reference substance, a rhein reference substance, a chrysophanol reference substance, a hesperidin reference substance, a costunolide reference substance, a dehydrocostunolide reference substance and a paeoniflorin reference substance, adding methanol to prepare a mixed reference substance solution, and filtering to obtain a reference substance solution; sucking the test sample solution and the reference substance solution, injecting the test sample solution and the reference substance solution into a high performance liquid chromatograph, measuring and recording a chromatogram, importing detection results of the test sample solution and the reference substance solution into fingerprint spectrum software, and generating a reference fingerprint spectrum through data matching. The quality of the fructus cannabis intestine moistening pill can be effectively represented, the quality of the medicine can be comprehensively monitored, and the method has the advantages of being high in stability, high in precision and good in reproducibility.

The invention relates to the technical field of medicine quality detection, in particular to a quality detection method for all medicines in Pingwei pills, which comprises the following steps: qualitatively identifying rhizoma atractylodis, mangnolia officinalis, pericarpium citri reticulatae and liquorice by adopting a thin layer chromatography (TLC), and simultaneously determining the contents of hesperidin, honokiol, magnolol and atractydin by adopting a high performance liquid chromatography (HPLC). The established method is easy and convenient to operate, good in repeatability and high in accuracy, qualitative identification of the whole medicine in the Pingwei pill and simultaneous determination of the content of four active ingredients in the monarch medicine, the minister medicine and the adjuvant medicine are achieved, and the quality control level of the Pingwei pill is improved.

The present application relates to the technical field of detection of phenolic acid, and discloses a quantitative detection method of phenolic acid components in rape root exudates based on UPLC-MS / MS, wherein the phenolic acid in the rape root exudates is p-hydroxybenzoic acid, vanillic acid, syringic acid, ferulic acid and hesperidin. The method comprises the following steps: (1) mixing freeze-dried rape root exudate powder with an extraction solvent, fully shaking and mixing, centrifuging the obtained mixture to obtain root exudate extract; (2) preparing a mixed mother liquor of p-hydroxybenzoic acid, vanillic acid, syringic acid, ferulic acid and hesperidin standard substances, and drawing a standard curve; (3) determining the root exudate extract prepared in step (1) in a UPLC-MS / MS multiple reaction monitoring mode according to the operation method and conditions of step (2). The method can be used for rapid quantitative detection of phenolic acid components in rape root exudates.

A bioplastic additive and antimicrobial plastic includes a polyphenol and an organic polymer. The polyphenol may be a phenol, polyphenol, bioflavenoid, flavonoid, tannin, coumarin, lagman, quinone, stibene or qurcuminoidstenin. The organic polymer may be chitosan, chitin, cellulose and keratin. In one advantageous form, the polyphenol is hesperidin and the organic biopolymer is chitosan.

The invention relates to an efficient preparation method of citrus flavone, hesperidin is converted into hesperidin chalcone which is better in solubility and more active, the hesperidin chalcone is subjected to halogenation, elimination, ring closing and refining to obtain a citrus flavone finished product, the yield is 90% or above and is 80% higher than that of a traditional citrus flavone synthesis method, the reaction process is safe, operation is easy and convenient, the yield is high, cost is low, and the method is suitable for industrial production. The method is suitable for industrial production.

The invention discloses a method for preparing a siniperca chuatsi flavored pet phagostimulant by using siniperca chuatsi byproducts, which comprises the following steps: performing microfiltration concentration-in-situ embedding treatment on siniperca chuatsi fermentation liquor to obtain a high-abundance liquid microsphere leavening agent; the method comprises the following steps: carrying out step-by-step enzymolysis process treatment on a smelly mandarin fish byproduct raw material to obtain a fermentation substrate with high hydrolysis degree; the fermentation substrate and a liquid microsphere leavening agent are subjected to mixed anaerobic fermentation, then microfiltration sterilization is conducted, and the liquid phagostimulant with the smelly mandarin fish flavor is obtained; microcapsules prepared from hesperidin / xanthan gum / corn starch are used for encapsulating the liquid phagostimulant, and the pet phagostimulant with the smelly mandarin fish flavor is obtained after freeze drying. The method is green and environment-friendly in the whole process, the utilization rate and bioavailability of mandarin fish byproducts are improved, customized production of the characteristic flavor of the smelly mandarin fish and long-period stable release of flavor compounds are achieved, hesperidin is introduced through embedding, the functional activity of the product is effectively enhanced, and the diversified requirements of current pet food are met.

The application discloses a preparation method of a new hesperidine dihydrochalcone-propylene aldehyde adduct, which comprises the following steps: adding new hesperidine dihydrochalcone into methanol to obtain mother liquor A; adding propylene aldehyde into methanol to obtain mother liquor B; mixing the mother liquor A and the mother liquor B, adding phosphate buffer solution, adjusting pH, heating and reacting, and filtering to obtain the new hesperidine dihydrochalcone-propylene aldehyde adduct. The new hesperidine dihydrochalcone-propylene aldehyde adduct prepared by the application can be used as a standard product of a propylene aldehyde reaction product in food detection, and the reaction system can generate the adduct within 5 min, and the clearance rate can reach 95.99% within 40 min. The new hesperidine dihydrochalcone-propylene aldehyde adduct can also be applied to qualitative determination of the propylene aldehyde adduct in rapid food detection. The preparation is simple, low in cost, efficient, free of consumption of a chromogenic agent, high in sample recovery rate, can effectively capture propylene aldehyde, and can control and effectively reduce the content of propylene aldehyde in food.

The utility model discloses a normal temperature neohesperidin reaction kettle convenient to sample and detect, which comprises a normal temperature neohesperidin reaction kettle main body, the left side of the normal temperature neohesperidin reaction kettle main body is fixedly connected with a convenient sampling tube, and the top of the convenient sampling tube is provided with a control valve. The left side of the normal-temperature neohesperidin reaction kettle main body is fixedly connected with a dustproof assembly, the dustproof assembly comprises a supporting plate, a motor, a first gear, a second gear, a screw rod, a transmission sleeve, a transmission block and a sealing cover, the supporting plate is fixedly connected to the left side of the normal-temperature neohesperidin reaction kettle main body, the motor is fixedly connected to the top of the supporting plate, and the first gear is fixedly connected to the top of the transmission sleeve. The first gear is fixedly connected to the output end of the motor. According to the reaction kettle sampling device, a convenient sampling detection function and a dustproof protection function are added, so that the reaction kettle sampling device has the effect of quickly sampling a reaction kettle for next-step work, and the condition that the sampling of the reaction kettle is relatively tedious, time-consuming and labor-consuming is prevented.

The invention belongs to the technical field of cosmetic production, and discloses a black eye composition based on microcirculation regulation-melanin metabolism double targeting and application thereof, and cosmetics, the black eye composition comprises aminobutyric acid, nicotinamide, acetyl tetrapeptide-5 and a synergist in a mass ratio of (15-25): (5-15): (0.01-0.2): (0.01-0.2); the synergist is selected from at least one of hesperidin methyl chalcone and glucosyl hesperidin, the composition can inhibit generation of dark circles from the perspective of reducing generation of melanin and relieving angiotension, and then the composition is suitable for the dark circle problem caused by multiple action mechanisms; in addition, after the synergist is added, the action effect of an action pathway for inhibiting melanogenesis or relieving angiotensin is further improved, and the dark circle removing capacity of the composition is further enhanced.

This invention discloses a method for fractional extraction of polysaccharides and flavonoids from dried tangerine peel using a pulsed electric field synergistic with cellulase. The method includes: first, pulverizing and sieving the dried tangerine peel; then, completely immersing the tangerine peel powder in distilled water; treating with a pulsed electric field; extracting in a water bath; filtering to obtain polysaccharides; and then subjecting the filter residue to a secondary extraction using cellulase to obtain highly active flavonoid components (hesperidin), while simultaneously collecting and recovering ethanol. This method is simple, highly efficient, and allows for solvent reuse. The pulsed electric field disrupts the cell walls of the dried tangerine peel, promoting polysaccharide dissolution and increasing the accessibility of the raw material for enzymatic hydrolysis, thus enhancing the subsequent extraction of hesperidin. This achieves highly efficient extraction of polysaccharides and flavonoid components (hesperidin) from dried tangerine peel. The resulting extract exhibits strong antioxidant, hypoglycemic, and lipid-lowering activities, showing potential application value in the fields of health products and functional foods.

The invention relates to the technical field of preparation of food packaging fresh-keeping materials, in particular to a carbon quantum dot-polyvinyl alcohol composite film as well as a preparation method and application thereof. The carbon quantum dot-polyvinyl alcohol composite film comprises polyvinyl alcohol, a plasticizer and hesperidin carbon quantum dots. The raw materials adopted by the invention are degradable or easy to recover, so that the environmental pollution is remarkably reduced. By converting waste agricultural and sideline resources into valuable carbon dots, the method has important value for realizing high value of the agricultural and sideline resources. The invention studies the effect of the hesperidin carbon quantum dots in the preservation aspect, and has important meanings for developing a novel preservation technology, prolonging the storage life of food and improving the preservation quality of the food.

The application relates to a method for recovering hesperidin and narirutin from hesperidin crystallization mother liquor, and belongs to the technical field of plant extraction, which comprises the following steps: S1, membrane filtration: hesperidin crystallization mother liquor is sequentially subjected to ceramic membrane and nanofiltration membrane filtration; S2, chromatographic separation: the nanofiltration membrane filtration liquid is subjected to chromatographic separation, 50-95% methanol aqueous solution is used as a gradient elution solvent, and eluate of 1-2 BV and eluate of 3-4 BV are collected respectively; S3, narirutin crystallization: the eluate of 1-2 BV is concentrated to a certain solid content, a crystallization solvent is added to perform cooling crystallization, and product narirutin is obtained; and S4, hesperidin crystallization: the eluate of 3-4 BV is concentrated to a certain solid content, a crystallization solvent is added to perform cooling crystallization, and product hesperidin is obtained. The method for recovering hesperidin and narirutin is simple and convenient, and the yield and content of the recovered product hesperidin and narirutin are high.

The invention relates to the field of wood coatings, in particular to a water-based LED light-cured stain-resistant and fingerprint-resistant coating and a preparation method thereof. The water-based LED photocuring stain-resistant and fingerprint-resistant coating is prepared from the following components in parts by weight: 20 to 30 parts of water-based organic silicon modified resin, 40 to 60 parts of water-based polyurethane resin, 10 to 20 parts of water-based acrylic resin, 8 to 15 parts of water-based fluororesin, 5 to 10 parts of UV monomer, 4 to 12 parts of photoinitiator, 8 to 15 parts of deionized water and 0.3 to 2 parts of water-based thickening agent, the photoinitiator comprises at least one of ginkgo flavone, hesperidin and soy isoflavone. Ginkgo flavone, hesperidin and soy isoflavone are used as photoinitiators, and a stable cross-linked network structure is formed through a photocuring process, so that a layer of hard and compact film is formed on the surface of the coating, the wear resistance and scratch resistance are improved, and attachment of dirt such as fingerprint grease can be effectively prevented.

The invention belongs to the technical field of traditional Chinese medicines, and particularly discloses a Qingjin cough-relieving decoction for preventing phlegm from forming and relieving cough and a quality detection method thereof. The Qingjin cough-relieving drink is prepared by decocting 12 medicinal materials including platycodon grandiflorum, schizonepeta, radix stemonae, cynanchum glaucescens, liquorice, pericarpium citri reticulatae, herba ardisiae japonicae and the like in water, and has the effects of ventilating the lung, relieving cough, reducing phlegm and relieving sore-throat. The quality detection method comprises the following steps: carrying out qualitative identification on pericarpium citri reticulatae, liquorice and cynanchum glaucescens in the Qingjin cough-relieving drink by adopting a thin-layer chromatography method, and determining the contents of hesperidin, bergenin, myricetin and gallic acid in the Qingjin cough-relieving drink by adopting a high-performance liquid chromatography method. The quality detection method is stable, feasible, high in specificity and accurate in detection result, and the quality of the Qingjin cough-relieving drink can be effectively and accurately evaluated and controlled by adopting the method. The extraction and preparation process of the Qingjin cough-relieving drink is optimized by adopting a Box-Behnken response surface method, and a quality detection method is established, so that technical support is provided for production and quality control of the Qingjin cough-relieving drink.