46 results about "Hesperidina" patented technology

The invention relates to a beauty and skin care enzyme and a preparation method thereof. According to the invention, fruits and traditional Chinese medicines are used as main fermentation raw materials, the fruits and the traditional Chinese medicines are crushed and mixed with fermentation strains, and active substances in each raw material are effectively retained through two-stage fermentation, filtration separation and freeze-drying treatment, SOD and sea buckthorn flavone contained in sea buckthorn fruits have the skin care effects of resisting oxidation and promoting collagen synthesis, and the sea buckthorn fruits have the skin care effects of resisting oxidation and promoting collagen synthesis. Salidroside contained in the rhodiola rosea has the skin care effects of resisting light aging and fatigue, astragalus polysaccharide and astragaloside contained in the astragalus membranaceus can repair a barrier and enhance immunity, ophiopogonin and polysaccharide contained in the radix ophiopogonis have the effects of preserving moisture and resisting saccharification, and hesperidin and nobiletin contained in the pericarpium citri reticulatae have the effects of resisting oxidation and adjusting grease secretion. The obtained compound enzyme has the effects of resisting oxidation, resisting inflammation and preserving moisture, has activity, stability and transdermal efficiency, and can be used in skin care products such as anti-oxidation essence, repair masks, late-up cream and the like.

The invention discloses a composition and application thereof, and the composition comprises synephrine or a pharmaceutically acceptable salt thereof, and further comprises naringin, neohesperidin and / or hesperidin. The composition prepared by compounding the synephrine or the pharmaceutically acceptable salt thereof with the naringin, the neohesperidin and / or the hesperidin has the effects of relieving, repairing, resisting wrinkles, easing pain, relieving itching or resisting allergy when being used as a functional component of a skin care product; when being used as a functional component of a scalp care product, the shampoo has the effects of removing dandruff, removing oil, relieving itching or preventing alopecia.

Compositions that contain flavonoids such as hesperidin, quercetin, hesperetin, and rutin and methods of treating viral diseases such as coronavirus (e.g., SARS COV-2) infection, influenza virus infection, rhinovirus infection, and human metapneumovirus infection.

The bioplastic additives and antimicrobial plastics include polyphenols and organic polymers. The polyphenol can be a phenol, a polyphenol, a bioflavonoid, a flavonoid, a tannin, a coumarin, a lagman, a quinone, a stilbene, or a qurcuminoidenin. The organic polymer may be chitosan, chitin, cellulose, and keratin. In an advantageous form, the polyphenol is hesperidin, and the organic biopolymer is chitosan.

The invention relates to an SNP (Single Nucleotide Polymorphism) site obviously related to sweet substances of Lithocarpus litseifolius leaves and application of the SNP site. The sweet substances comprise trilobatin, phlorizin, 3-hydroxy phlorizin, neohesperidin dihydrochalcone, phloretin and general flavone. The total number of the SNP loci obviously related to different sweet substances provided by the invention is 84, and the physical positions of the SNP loci are compared and determined according to a reference genome with the login number of GCA040182985.1. The specific SNP site physical position and variation information are shown in a table 5. The loci can be further utilized to establish a molecular marker-assisted selection technology to help breeders to quickly screen high-sweetness Lithocarpus litseifolius strains, so that the traditional breeding period is shortened, and the breeding efficiency is improved. The resource guarantee is provided for the development of a novel Lithocarpus litseifolius sweetener, and the reference is also provided for germplasm innovation, variety specificity identification, quality improvement and genetic evolution relationship discussion of excellent Lithocarpus litseifolius.

The invention discloses a determination method for a dissolution curve of a citrus flavone tablet, and belongs to the technical field of pharmaceutical analysis. The method comprises the following steps: setting dissolution conditions, and preparing a dissolution medium which is a strong alkali solution with the mass volume ratio of 0.1-0.6% or a strong alkali and weak acid salt solution with the mass volume ratio of 1-2% and a Tween 80 solution with the mass volume ratio of 1-2%; the method comprises the following steps: setting liquid chromatography detection conditions, and adopting a Phenomenex Gemini chromatographic column; preparing a test solution and a reference solution; and finally, detecting the contents of diosmin and hesperidin in each solution according to a liquid chromatography to obtain dissolution rates at different time, and establishing a dissolution curve according to the dissolution rates and the dissolution time. The method has the advantages of scientificity, durability and reproducibility, the measured dissolution curve has distinguishing power, the in-vitro release behavior of the dissolution curve can be evaluated, guarantee is provided for quality consistency between batches of drugs, the method can also be used for quality consistency evaluation work of imitated drugs and originally developed preparations, and the medication curative effect is guaranteed.

The invention discloses a twice crystallization method for extracting hesperidin, and belongs to the technical field of plant extraction. The method for extracting hesperidin comprises the following steps: cleaning and crushing a raw material rich in hesperidin; adding an impurity removal solvent, and carrying out impurity removal reaction; adding an extraction solvent into the raw materials subjected to impurity removal to carry out extraction reaction, wherein the extraction solvent is a mixed solution of low-level saturated monohydric alcohol with the volume percent of 40-80% and alkali with the mass percent of 0.3-1.0%; adding acid into the extracting solution to adjust the pH value to 7.5-8.5; carrying out a first crystallization reaction, adding acid into the residual mother liquor to adjust the pH value to 6.5-7.5, and carrying out a second crystallization reaction; the extraction, purification and recovery processes of the hesperidin are optimized through a two-time crystallization method of sequentially performing crystallization in an alcohol system and a water system. The method not only overcomes the defects of large wastewater discharge amount and complex process of the traditional extraction process, but also further improves the extraction yield of hesperidin, reduces the production cost, and makes the extraction process more efficient and environment-friendly.

To provide a composition for improving blood vessel repair function using cells.SOLUTION: This composition for improving blood vessel repair function comprises: at least one selected from the group consisting of astaxanthin, glucosamine hydrochloride, chondroitin sulfate, tocopherol, resveratrol, hesperidin (vitamin P), beta-carotene, ubiquinone, lipoic acid, zinc, arsine, arginine, citrulline, taurine, vitamin E, and mucin; and stem cells.SELECTED DRAWING: Figure 1

The invention relates to a construction method of a fingerprint spectrum of Shenxiong Tongmai capsule, which comprises the following steps: dissolving a ferulic acid standard substance, a hesperidin standard substance, a ginsenoside Re standard substance, a neohesperidin standard substance and a naringin standard substance in methanol to prepare a mixed reference substance solution; the method comprises the following steps: respectively dissolving contents of Shenxiong Tongmai capsules in a plurality of batches in a methanol aqueous solution to prepare a plurality of test solutions; respectively carrying out high performance liquid chromatography detection on the mixed reference substance solution and the plurality of test sample solutions to obtain a high performance liquid chromatogram of the mixed reference substance solution and high performance liquid chromatograms of the plurality of test sample solutions; determining characteristic peaks and identifying chemical components of the characteristic peaks, and calculating relative retention time of other characteristic peaks by taking the characteristic peak corresponding to hesperidin as a reference peak to obtain the fingerprint spectrum. The fingerprint spectrum obtained by the construction method disclosed by the invention can well reflect information of a plurality of index components of the Shenxiong Tongmai capsule.

The application provides a method for detecting the pharmacodynamic components of a traditional Chinese medicine composition by UPLC-MS / MS. The method can identify 14 pharmacodynamic components of the traditional Chinese medicine composition, including saikosaponin A, saikosaponin D, naringin, paeoniflorin, hesperidin, chicoric acid, chlorogenic acid, salvianolic acid B, zephyranthine, kaempferitrin, and codariusrhizome glycoside, amargin, hyperoside and rutin, thereby providing a reference for evaluating the intrinsic quality of the traditional Chinese medicine composition.

A colorimetric card for rapidly detecting hesperidin concentration, as well as its preparation method and application, relates to the technical field of hesperidin concentration detection. A colorimetric card for rapidly detecting hesperidin concentration is provided. The colorimetric card is a collection of color development degrees obtained by developing hesperidin solutions of a certain gradient concentration in a fluorescent support medium under ultraviolet light. The colorimetric card can be compared with the color development degree obtained by developing a hesperidin solution extracted from tangerine peel to be tested in a fluorescent support medium under ultraviolet light, thereby rapidly determining the concentration range of the hesperidin solution and thereby determining the quality of the tangerine peel.

The present application relates to the technical field of food packaging preservation material preparation, and particularly relates to a carbon quantum dot-polyvinyl alcohol composite film and a preparation method and application thereof. The carbon quantum dot-polyvinyl alcohol composite film comprises polyvinyl alcohol, a plasticizer and hesperidin carbon quantum dots. The raw materials used in the present application are degradable or easy to recycle, which significantly reduces environmental pollution. By converting waste agricultural and sideline resources into valuable carbon dots, it has important value for realizing the high value of agricultural and sideline resources. The present application studies the role of hesperidin carbon quantum dots in preservation, which has important significance for developing new preservation technology, improving food storage life and preservation quality.

The invention discloses saccharomyces cerevisiae and a method for efficiently synthesizing neohesperidin by using cane sugar. According to the saccharomyces cerevisiae, on the basis of ZQH01, FjTAL derived from Flavobacterium johnsoniae, AtC4H, AtPAL2 and AtATR2 derived from Arabidopsis thaliana and CYB5 derived from yeast are inserted into a genome of the ZQH01, and the saccharomyces cerevisiae is prepared by the following steps of: adding the FjTAL, the AtC4H, the AtPAL2 and the AtATR2 into the genome of the ZQH01; according to the invention, a sucrose phospholysis double-carbon-source system is introduced into the saccharomyces cerevisiae, sucrose can be directly converted into fructose and glucose-1-phosphoric acid, the fructose is specially used for cell growth and hesperetin synthesis, the glucose-1-phosphoric acid directionally supplies UDP-glucose, and a carbon flow is physically separated to eliminate flux competition caused by a Crab effect. The saccharomyces cerevisiae disclosed by the invention can be used for efficiently synthesizing neohesperidin by using cane sugar, and has important significance on green and efficient acquisition of citrus flavonoids.

The objective of this invention is to provide a beverage containing dead lactic acid bacteria, in which the off-flavor of the culture medium for lactic acid bacteria is suppressed, and a method for manufacturing the same. This objective is achieved when the concentration of dead lactic acid bacteria (one or more) at 1.3 billion cells / L or higher, and the concentration of hesperidin at 10–1000 mg / L.

This invention discloses a method for extracting hesperidin, belonging to the field of natural product extraction technology. The method includes the following steps: S1, pulverizing and sieving the raw material to obtain raw material powder; mixing the raw material powder with an extractant and performing ultrasonic-assisted extraction to obtain an extract; concentrating the extract to obtain crude hesperidin; S2, adding ethanol solution to the crude hesperidin extract, allowing it to stand, and filtering to obtain an alcohol extract; S3, adjusting the pH of the alcohol extract to acidic using acid, stirring, filtering, and collecting the precipitate; S4, dissolving the precipitate with an alkaline solution, filtering, adjusting the pH of the filtrate to acidic using acid, precipitating the precipitate, and drying to obtain crude hesperidin; S5, dissolving the crude hesperidin in ethanol solution, purifying it using macroporous resin, and freeze-drying to obtain pure hesperidin. This extraction method maintains the structural integrity of hesperidin and achieves high extraction rate and high purity.

The present invention belongs to the technical field of pharmaceutical formulations. Specifically, provided is a method for preparing a traditional Chinese medicine nano-micelle formulation encapsulating hesperidin. The specific steps and process of the method for preparing the traditional Chinese medicine nano-micelle formulation encapsulating hesperidin are as follows: a polyethylene glycol-polylactic acid block copolymer, hesperidin, dichloromethane and poloxamer 188 are selected for later use. According to the present application, the traditional Chinese medicine nano-micelle formulation encapsulating hesperidin is prepared by means of mixing the polyethylene glycol-polylactic acid block copolymer, hesperidin, dichloromethane and poloxamer 188. The preparation process is simple and involves single raw material selection, such that the production cost is effectively controlled. The preparation method is suitable for industrial large-scale production and can be widely used in clinical practice.

The application belongs to the technical field of content detection, and discloses a kind of HPLC detection methods for simultaneously determining the content of multiple components in semen Cannabis capsules.The detection method includes mixing reference solution, chrysophanol and methanol to obtain mixed reference solution.The reference solution includes physcion solution, magnolol solution, houpo chrysophanol solution, emodin solution, rhein solution, nobiletin solution, aloe emodin solution, hesperidin solution and paeoniflorin solution;The contents of semen Cannabis capsules are mixed with methanol, and the test solution is obtained after heating and refluxing;The mixed reference solution and test solution are respectively sucked and injected into high performance liquid chromatograph.The detection method can simultaneously determine the content of multiple index components, can comprehensively reflect the quality of the preparation, and the test method has excellent precision, repeatability, stability and high sample recovery rate.

The present invention relates to flavonoids for use in improving sleep quality. The flavonoids of the invention are naringin or naringenin and hesperidin. The compounds are particularly useful in decreasing sleep onset latency, decreasing sleep latency, improving sleep efficiency, decreasing sleep disturbance, decreasing wake after sleep onset, decreasing the number of awakenings, improving the ratio of sleep stages, decreasing medication use, decreasing daytime dysfunction, feeling rested / refreshed, and feeling sleepy after waking up.

The present invention discloses a method for constructing a fingerprint of a tangerine seed and a traditional Chinese medicine preparation thereof and its application, and relates to the technical field of traditional Chinese medicine identification. The method for constructing a fingerprint of a tangerine seed and a traditional Chinese medicine preparation thereof comprises: extracting the tangerine seed or the traditional Chinese medicine preparation thereof with a first extraction solvent to obtain a test solution; dissolving or extracting a reference substance and / or a tangerine seed reference medicinal material with a second extraction solvent to obtain a reference substance solution and / or a reference medicinal material solution; measuring the test solution, the reference substance solution and / or the reference medicinal material solution with a liquid chromatograph to obtain a fingerprint of the tangerine seed or the traditional Chinese medicine preparation thereof; wherein the chromatographic column of the liquid chromatograph uses octadecylsilane bonded silica gel as the stationary phase, acetonitrile as the mobile phase A, and a phosphoric acid aqueous solution with a concentration of 0.1 vol% to 0.5 vol% as the mobile phase B for gradient elution, and the reference substances include a vecinin-2 reference substance, a hesperidin reference substance, a limonin reference substance, a nomilin reference substance, and a flavonoid reference substance; the first extraction solvent and the second extraction solvent are methanol aqueous solutions with a concentration of 30 vol% to 100 vol%. The fingerprints constructed by the present invention contain rich characteristic peak information, enabling identification from common counterfeits. Furthermore, the construction method is simple, stable, and highly specific, making it applicable to various types of tangerine seed slices and their standard decoctions and granules.

The present application relates to a kind of traditional Chinese medicine composition containing heat-sensitive component and its preparation method and application, the preparation method of the traditional Chinese medicine composition containing heat-sensitive component of the present application does not use a large amount of anhydrous ethanol for alcohol precipitation, avoid the loss of polysaccharide active ingredient precipitation, also does not use high temperature extraction exceeding 80 DEG C, avoid the loss of heat-sensitive component such as hawthorn acid and hesperidin.The preparation method of the present application combines enzyme engineering, countercurrent kinetics, supercritical fluid and other technologies, while ensuring the extraction efficiency, reduce the loss of heat-sensitive component such as hawthorn acid and hesperidin and sugar active ingredient, improve extraction efficiency and reduce energy consumption cost, and the amount of anhydrous ethanol is reduced by more than 70%, in line with GMP clean production standard.

To provide a technology capable of inhibiting an unpleasant taste such as bitterness, in a food composition including naringin, without impairing an original flavor inherent to the food.SOLUTION: The present invention provides an agent for inhibiting a bitter taste derived from naringin, the agent comprising epigallocatechin gallate as an active ingredient. The bitter taste inhibiting agent can be added to a food composition having a bitter component including naringin in order to inhibit the bitterness.SELECTED DRAWING: None

The invention belongs to the field of traditional Chinese medicine analysis, and particularly relates to a method for determining the content of Zhuanshu granules by combining a specific chromatogram with multiple indexes. According to the method, a high performance liquid chromatography method is adopted, acetonitrile is taken as a mobile phase A, a 0.05-0.2% phosphoric acid aqueous solution is taken as a mobile phase B, the gradient elution condition is 0 min, the mobile phase A accounts for 14-16%, and the mobile phase B accounts for 84-86%; the mobile phase A accounts for 18-22%, and the mobile phase B accounts for 78-82%; the mobile phase A accounts for 38-42% and the mobile phase B accounts for 58-62% in 25 min, and the mobile phase A accounts for 38-42% and the mobile phase B accounts for 58-62% in 40 min. The specific chromatogram of 13 chromatographic peaks with hesperidin as a reference substance is established for the first time, qualitative and quantitative evaluation of multiple medicine flavors in the prescription is achieved at the same time, operation is easy and convenient, the cost is reduced, the efficiency is improved, and the overall quality control level of the Zhuanshu granules is improved.

This invention relates to a complex method for improving the solubility of hesperidin in concentrated orange juice, belonging to the field of food processing technology. Specifically, it relates to a technique that combines specific functional components and a physical field to efficiently and stably improve the solubility of hesperidin in concentrated orange juice. The main steps of this invention include: raw material selection, preparation of concentrated orange juice, preparation of a functional polysaccharide-EGCG composite cosolvent, addition of the functional polysaccharide-EGCG composite cosolvent, ultrasonic treatment, sterilization, static magnetic field-assisted cooling, and filling. The functional polysaccharide-EGCG composite cosolvent is synthesized by the self-assembly of functional polysaccharides and epigallocatechin gallate (EGCG); the static magnetic field-assisted cooling involves applying a low-temperature static magnetic field to the concentrated orange juice after pasteurization. Compared with concentrated orange juice prepared by untreated or single-treatment methods, this invention can significantly improve the solubility of hesperidin in concentrated orange juice, contributing to the quality improvement of such products.

The present invention belongs to the technical field of pharmaceutical formulations. Specifically, provided is a method for preparing a traditional Chinese medicine nano-micelle formulation encapsulating hesperidin. The specific steps and process of the method for preparing the traditional Chinese medicine nano-micelle formulation encapsulating hesperidin are as follows: a polyethylene glycol-polylactic acid block copolymer, hesperidin, dichloromethane and poloxamer 188 are selected for later use. According to the present application, the traditional Chinese medicine nano-micelle formulation encapsulating hesperidin is prepared by means of mixing the polyethylene glycol-polylactic acid block copolymer, hesperidin, dichloromethane and poloxamer 188. The preparation process is simple and involves single raw material selection, such that the production cost is effectively controlled. The preparation method is suitable for industrial large-scale production and can be widely used in clinical practice.

The invention belongs to the technical field of pericarpium citri reticulatae production, and particularly relates to pericarpium citri reticulatae and a production process thereof.The production process comprises the following steps that S1, materials are prepared, mandarin orange peel, pericarpium citri reticulatae fruits and water are prepared; s2, preparing soup, namely washing and crushing dried orange peel fruits, and soaking the crushed dried orange peel fruits and water in a vacuum pot to form the soup; and S3, blanching the mandarin orange peels: blanching the mandarin orange peels in hot water, and then fishing out the blanched mandarin orange peels. S4, stirring and heat preservation: putting the mandarin orange peel into the vacuum pot in the step S2, uniformly stirring, heating, opening small steam for heat preservation, preserving heat for seven days, and stirring once a day. And S5, spin-drying the prepared dried orange peel, and then drying. According to the present invention, the tangerine peel is soaked with the tangerine peel fruit liquid, such that the more complex aroma layer of the tangerine peel prepared from the tangerine peel can be added, the method is suitable for cooking, tea drinking and other use methods, and in the aging process, the flavonoid substances in the tangerine peel, such as hesperidin, have the anti-oxidation effect, and after the tangerine peel is soaked, the active components in the tangerine peel fruit liquid can be absorbed so as to achieve the anti-oxidation effect; the antioxidant activity is increased, the antioxidant capacity is enhanced, and free radicals can be removed easily.

The application discloses a method for preparing pet food attractant with the flavor of stink mandarin fish by using mandarin fish by-products, and comprises the following steps: subjecting stink mandarin fish fermentation liquor to microfiltration concentration-in-situ embedding treatment to obtain high-abundance liquid microsphere leavening agent; subjecting stink mandarin fish by-product raw materials to step-by-step enzymatic hydrolysis process treatment to obtain high-hydrolysis-degree fermentation substrate; mixing the fermentation substrate with the liquid microsphere leavening agent to perform anaerobic fermentation, and then removing bacteria by microfiltration to obtain liquid food attractant with the flavor of stink mandarin fish; using hesperidin / xanthan gum / corn starch to prepare microcapsules for encapsulating the liquid food attractant, and obtaining pet food attractant with the flavor of stink mandarin fish after freeze-drying. The method is green and environmentally friendly throughout, improves the utilization rate and bioavailability of mandarin fish by-products, realizes the customized production of the characteristic flavor of stink mandarin fish, and realizes the long-period stable release of flavor compounds, and the introduction of hesperidin through embedding effectively enhances the functional activity of the product, and meets the diversified needs of pet food at present.

The invention provides a separation method of a neohesperidin finished product, and belongs to the technical field of separation and purification. In the implementation process, a whole set of separation treatment including alkali dissolution, membrane separation, acid precipitation, concentration, cooling crystallization and centrifugal separation is carried out on the neohesperidin obtained after fermentation is finished, the yield of the obtained neohesperidin finished product is about 85%, and industrialization of the neohesperidin fermentation process is achieved.

A fructus cannabis intestine-moistening pill high performance liquid chromatography fingerprint establishment method relates to the field of medicine, and comprises the following steps: weighing fructus cannabis intestine-moistening pill medicinal material powder, adding methanol, plugging, weighing, soaking, carrying out ultrasonic extraction, cooling, weighing, complementing the weight loss, uniformly shaking, and filtering to obtain a test solution; the method comprises the following steps: weighing an emodin reference substance, a rhein reference substance, a chrysophanol reference substance, a hesperidin reference substance, a costunolide reference substance, a dehydrocostunolide reference substance and a paeoniflorin reference substance, adding methanol to prepare a mixed reference substance solution, and filtering to obtain a reference substance solution; sucking the test sample solution and the reference substance solution, injecting the test sample solution and the reference substance solution into a high performance liquid chromatograph, measuring and recording a chromatogram, importing detection results of the test sample solution and the reference substance solution into fingerprint spectrum software, and generating a reference fingerprint spectrum through data matching. The quality of the fructus cannabis intestine moistening pill can be effectively represented, the quality of the medicine can be comprehensively monitored, and the method has the advantages of being high in stability, high in precision and good in reproducibility.

The invention relates to the technical field of medicine quality detection, in particular to a quality detection method for all medicines in Pingwei pills, which comprises the following steps: qualitatively identifying rhizoma atractylodis, mangnolia officinalis, pericarpium citri reticulatae and liquorice by adopting a thin layer chromatography (TLC), and simultaneously determining the contents of hesperidin, honokiol, magnolol and atractydin by adopting a high performance liquid chromatography (HPLC). The established method is easy and convenient to operate, good in repeatability and high in accuracy, qualitative identification of the whole medicine in the Pingwei pill and simultaneous determination of the content of four active ingredients in the monarch medicine, the minister medicine and the adjuvant medicine are achieved, and the quality control level of the Pingwei pill is improved.

The present application relates to the technical field of detection of phenolic acid, and discloses a quantitative detection method of phenolic acid components in rape root exudates based on UPLC-MS / MS, wherein the phenolic acid in the rape root exudates is p-hydroxybenzoic acid, vanillic acid, syringic acid, ferulic acid and hesperidin. The method comprises the following steps: (1) mixing freeze-dried rape root exudate powder with an extraction solvent, fully shaking and mixing, centrifuging the obtained mixture to obtain root exudate extract; (2) preparing a mixed mother liquor of p-hydroxybenzoic acid, vanillic acid, syringic acid, ferulic acid and hesperidin standard substances, and drawing a standard curve; (3) determining the root exudate extract prepared in step (1) in a UPLC-MS / MS multiple reaction monitoring mode according to the operation method and conditions of step (2). The method can be used for rapid quantitative detection of phenolic acid components in rape root exudates.