94 results about "Hesperidina" patented technology

The invention discloses a method for preparing neohesperidin by an enzymic method, comprising the following steps: performing coordination between hesperidin and metal ions and dissolving in water, and realizing conversion from hesperidin to neohesperidin under the action of immobilized rhamnosidase and immobilized rhamnoside transferase. The method provided by the invention is adopted to solve the problem that production cost of neohesperidin widely used in medical, functional food and its cosmetic fields is high, and simultaneously opens up a new approach for recycling of industrial waste orange peel and high value-added utilization of hesperidin.

The invention relates to the technical field of plant extraction and provides a method for extracting hesperidin from fructus aurantii immaturus according to a continuous countercurrent method and theextracted hesperidin. The method for extracting hesperidin from fructus aurantii immaturus includes: providing fructus aurantii immaturus infiltrated by alkaline alcoholic solution, mixing with alkaline alcoholic solution, performing continuous countercurrent extraction, and crystallizing extract liquid obtained in continuous countercurrent extraction to obtain hesperidin. The method is high in extraction efficiency, extraction time can be effectively shortened, and accordingly productivity is improved; in addition, extraction solvent quantity can be effectively decreased, and accordingly alcohol consumption and alcohol recovery energy consumption are sharply reduced, and cost reduction is realized; moreover, the extract liquid is high in product concentration, concentration difference ofextract liquid in different time periods is avoided, and stable product quality is realized after crystallization. The hesperidin is obtained by application of the method for extracting hesperidin from fructus aurantii immaturus. The hesperidin has advantages of high purity and quality stability and is high in market value and worthy of popularization and application.

The invention discloses a preparation method of pericarpium citri reticulatae absolute oil. The pericarpium citri reticulatae absolute oil is obtained through the steps of pericarpium citri reticulatae crushing, pericarpium citri reticulatae fermenting, pericarpium citri reticulatae extracting, extracting and filtering, concentrating, alcohol precipitating and concentrating. According to the obtain pericarpium citri reticulatae absolute oil, the content of flavonoids such as hesperidin, nobiletin and tangeretin and the content of volatile oil such as limonene are high, therefore, the unique aroma of pericarpium citri reticulatae is effectively kept, and the natural flavor is clearer when the pericarpium citri reticulatae absolute oil is applied to the cigarettes; in addition, the application range of the absolute oil can be wider, the state and solubility problems of a pericarpium citri reticulatae extract are solved, and the absolute oil can be better applied to the cigarettes; the preparation process is simple, the operability is high, the whole production process is efficient, and the method is suitable for industrialized implementation; on the other hand, when the pericarpium citri reticulatae absolute oil prepared through the method is applied to the cigarettes, the mouthfeel and the aftertaste of cigarette smoking can be improved, the special natural flavor can be given to the cigarettes, and the smoke quality can be promoted.

The invention discloses alpha-glucose-based new hesperidin dihydrochalcone. The compound is obtained by carrying out alpha-glucose-based reaction by using new hesperidin dihydrochalcone and dextrin as raw materials under the catalysis of glucosyltransferase. The alpha-glucose-based new hesperidin dihydrochalcone synthesized by the method can be widely used for the industries of foodstuff, medicaments, forages, daily chemicals and the like as a sweetener, a flavoring agent, a flavor enhancer and the like.

The invention relates to an oral care composition containing flavonoid. The composition is characterized by being prepared from 10 parts of luteolin, 10 parts of baicalein, 5 parts of kaempferol, 20 parts of hesperidin, 20 parts of hesperidin and 0.2-2 parts of peppermint oil. The composition has the advantages that antibiosis, anti-inflammatory, spasmolysis and immunity improvement functions are realized, the side effect is small, the absorption rate is high, and mouthfeel is good. The composition can be used for oral care of critical patients and treatment of patients suffering from dental decay, periodontitis and stomatitis for a long time.

The invention discloses a method for preparing hesperetin monoglucoside by fermentation, enzymolysis, extraction and coupling, belonging to the technical field of biochemical substances extraction. The method comprises the following steps: preparing a nutrient solution; preparing a strain seed solution; preparing fermentation liquor for producing the hesperetin monoglucoside; culturing the fermentation liquor for producing the hesperetin monoglucoside; carrying out resin adsorption, back flow, discharging and feeding on the fermentation liquor; and eluting and collecting resin. According to the method disclosed by the invention, the Fusarium nivale HESPER strain CGMCCNo.6238 is adopted as a culture, and the hesperetin monoglucoside is prepared by continuous culture fermentation, hesperidin enzymolysis, extraction and coupling, thus the hesperetin monoglucoside is effectively prevented from being degraded into hesperetin, the yield is enhanced, and the production cost is reduced. The method disclosed by the invention can be popularized and applied to related areas and enterprises.

The invention belongs to the field of cosmetics and particularly relates to a skin-whitening, anti-aging and anti-allergic hesperidin composition, an application and a preparation. According to the composition containing hesperidin and pectolinarin, provided by the invention, the number of melanin particle-containing positive cells and the content of tyrosinase can be remarkably reduced, the SOD activity can be remarkably improved, the MDA content can be reduced, and the ear swelling and the licking frequency of an experimental animal can be remarkably reduced; and the hesperidin and the pectolinarin are compounded to achieve the effects of remarkably whitening skin, resisting aging and resisting allergy.

The invention provides an edible low-calorie rare sugar sweetening agent composition. Specially, trehalose, erythritol, neohesperidin dihydrochalcone and steviol glycoside are compounded in a certainproportion, so that a sweetening agent composition being good in homogeneity, low in calorie, good in flavor and good in stability can be obtained. The sweetening agent composition has the characteristics of being pure in mouth feel and refreshing, and also has the efficacy of being low in calorie, balanced in blood sugar and high in stability, containing prebiotics, preventing dental caries, andthe like.

The present invention provides a multiple index component and fingerprint determination method of a liver-tonifying eyesight-improving oral liquid, and the method aims at chlorogenic acid, luteoloside, ferulic acid, nobiletin, paeoniflorin and hesperidin. The determination method is simple, strongly-specific, good in stability, high in precision, good in reproducibility and high in sample recovery rate. Further, the present invention also provides a preparation method of the liver-tonifying eyesight-improving oral liquid and the liver-tonifying eyesight-improving oral liquid prepared by the preparation method, and the preparation method comprises the step of the determination method.

The invention relates to the field of extraction and preparation of natural components of plants, relates to a natural product extraction method and in particular relates to a method for extracting hesperidin. The method comprises the following steps: by taking an alkali solution as a solvent, extracting an immature bitter orange raw material so as to obtain an extract; and adjusting the pH valueof the extract to acid by using citric acid to generate a crystal in the extract, so as to obtain a hesperidin crystal. By adopting the method, hydrochloric acid is replaced by an organic acid to crystallize and precipitate hesperidin, a hesperidin product of which the quality meets requirements can be obtained, in addition, the production process is environmentally friendly and safe, and the quality of the product can be improved. The scheme can be applied to practical operation of industrial extraction and purification separation of the hesperidin.

The invention relates to a semi-synthesis method of luteolin and galuteolin as well as luteolin rutinoside by using hesperidin, a semi-synthesis method of the galuteolin by using hesperetin glucoside, and a semi-synthesis method of the luteolin by using hesperetin, and belongs to the field of chemistry and medicines. The semi-synthesis method comprises the following steps: enabling the hesperidin, the hesperetin glucoside and the hesperetin to be subjected to complexation in pyridine alcohol fluid, dehydrogenizing by using iodine, directly distilling alcohol and pyridine, maintaining for a period of time in an airtight distilled state, and carrying out demethylation reaction, so that the hesperidin is generated into the luteolin rutinoside; generating the galuteolin by demethylation of diosmetin glucoside, and generating the luteolin by diosmetin; and hydrolyzing the luteolin rutinoside, so that the luteolin rutinoside is transformed into luteolin and galuteolin. The semi-synthesis method has the advantages that two steps of dehydrogenation and demethylation are combined into one step, and reaction conditions of dehydrogenation and demethylation are mild and easy to control; few reagents are used and green and environmentally friendly; and the demethylation yield is high, and the industrial production is easy. Compared with disclosed documents and patients, the semi-synthesis method has great advantages in the production of luteolin and glucosides of the luteolin.

The invention provides a continuous flow microchannel synthesis process of flavonoid compounds. According to the process, hesperidin and iodine elementary substance are used as raw materials and react in a continuous flow microchannel reactor in the presence of a reaction solvent to synthesize the flavonoid compound as shown in a formula A. Compared with a traditional kettle-type preparation process, the process disclosed by the invention has the advantages that the preparation time is obviously shortened, and the conversion rate of raw materials and the yield of products are obviously improved; and especially, when the diosmin is prepared under optimal process conditions of continuous flow microchannel synthesis, the conversion rate of the raw material hesperidin is as high as 96.48%, and the yield of the product diosmin is as high as 81.96%. The continuous flow micro-channel synthesis process provided by the invention is beneficial to realizing safe, efficient and rapid industrial production of flavonoid compounds, and has a wide application prospect.

The invention relates to a quality control method for a liver-soothing qi-regulating pill as traditional Chinese preparation, which is prepared from traditional Chinese herbal medicines as raw materials, belonging to the technical field of Chinese patent medicine; the method comprises the steps that: with magnolol, honokiol and cyperone as reference substances, thin-layer chromatography is adopted to identify whether the formula of the liver-soothing qi-regulating pill contains the ingredients of magnolol, honokiol and cyperone; and with hesperidin as a reference substance, high efficiency liquid chromatography is adopted to detect the content of the hesperidin in the formula of the liver-soothing qi-regulating pill. The revised quality standard is more perfect and the quality control for medicines is improved.

The invention relates to a clean preparation process of high-purity hesperetin. The process mainly comprises the following steps: (1) hydrolyzing hesperidin to obtain the hesperetin, (2) concentrating hesperetin reaction liquid in a vacuum mode, (3) depositing the hesperetin concentrated solutions, (4) preparing the hesperetin, and (5) processing filter pressing acid water solutions. According to the process, hesperetin which is more than 95% in content (high performance liquid chromatography (HPLC)) is obtained through a further reaction and a special processing process. Compared with the facts that in prior art, a crude product is firstly obtained through hydrolyzing, and then a fine product is obtained by refining the crude product, the clean preparation process of high-purity hesperetin has the advantages of being simple in process, low in cost, and high in yield. Acid water which is generated by hydrolyzing is well processed and recycled, rhamnose in the acid water is abstracted, and crystallization mother liquor is prepared into agricultural nucleic acid. Therefore, the whole production process is resource-saving and environment-friendly.

A combination composition comprising as active ingredients L-carnitine or propionyl L-carnitine, troxerutine, diosmine and hesperidine, useful for the prevention and / or treatment of chronic venous diseases.

The invention relates to the field of natural product preparation, and relates to a comprehensive utilization method of immature bitter oranges. According to the method, the step of enzymolysis pretreatment is added, so that the hesperidin extraction efficiency is improved, the extraction time is shortened, and the amount of an extraction solvent is reduced, the damage to functional components inthe long-time extraction process is avoided, and the comprehensive utilization of immature bitter oranges can be realized. The comprehensive utilization method can be applied to production practice ofextraction of hesperidin, the extraction rate and purity of hesperidin are improved, and the comprehensive utilization rate of the raw material immature bitter oranges is further guaranteed.

A carbonated beverage comprises the following raw materials per 1000mL: 2-3g citric acid, 0.2-0.8g sodium citrate, 5-10g fructo-oligosaccharide, 8-12mg neohesperidin dihydrochalcone and 0.2-1g pigment. A carbon dioxide content is not less than 3%. The carbonated beverage is prepared by water treatment, chilled water treatment, batching, premixing, filling, sealing, code spraying, light inspection, temperature return, labeling, water removal, inspection, warehousing and the like. In the carbonated beverage, fructo-oligosaccharide and neohesperidin dihydrochalcone serve as sweetening agents, so that a synergistic effect is enhanced, and a taste is mediated. The carbonated beverage is simple in process and good in taste, has multiple health-care effects at the same time, is an ideal fashionable drink, and is suitable for various human bodies with diabetes and adiposity.

The invention relates to a method for determining the content of index components in a fingered citron preparation. The method comprises the following steps: step 1, preparing a mixed reference solution and a test solution; 2, carrying out high performance liquid chromatography detection; 3, establishing three index component standard curves; and 4, determining the content of three index components of geraniin, hesperidin and 5, 7dimethoxycoumarin in the test solution. The method simultaneously detects three index components of geraniin, hesperidin and 5, 7dimethoxycoumarin in the fingered citron preparation, is accurate and efficient, can be used for further quality control of the fingered citron preparation, has the advantages of accurate detection result, high precision and good repeatability, Compared with the existing determination method, the method can better reflect the content of effective components of fingered citron and related preparations.

The invention discloses a method for producing diosmin. The method for producing the diosmin comprises the following production procedures of: performing a main reaction; cleaning; purifying; drying; grinding and mixing; and recovering iodine. According to the technical scheme, the conversion rate of an organic raw material hesperidin is over 95 percent; yield and content are high; the iodine, pyridine, methanol and ethanol can be recovered and reused on line and the recovery rate is 90 percent; material consumption and production cost are low; and the method has the advantages of simple technological process and stable process.

The invention discloses a low-cost preparation method of diosmin. The low-cost preparation method of diosmin comprises the following steps: dissolving hesperidin and iodine in pyridine; performing heating and introducing ozone until the concentration of hesperidin is lower than 5%; adjusting the pH value to be neutral, and performing filtering to obtain a first filtered substance; washing the first filtered substance with deionized water, adding alkali for dissolving, and performing filtering to obtain filtrate; and acidizing and recrystallizing the filtrate to obtain diosmin. The method has the effects of reducing the dosage of iodine and lowering the cost.

The invention discloses an extraction method of an immature bitter orange extract. The extract comprises pectin, synephrine, hesperidin, total flavonoids of immature bitter oranges, plant fibers and the like. The extraction method comprises the steps: selecting an immature bitter orange raw material with a diameter of less than 1.5 cm; extracting with a hydrochloric acid solution to obtain a mixture of pectin and synephrine, and further separating and purifying to obtain pectin and synephrine products; extracting with a sodium carbonate solution to obtain an immature bitter orange total flavone product; extracting with sodium hydroxide to obtain a hesperidin product; and the finally extracted residues are pure plant fibers. Various products are comprehensively developed from immature bitter oranges by using various methods, and the complete utilization of materials is ensured so that the production cost is reduced, and the environmental pollution is reduced.

The invention discloses a steaming processing method of dried tangerine or orange peel decoction slices. The steaming processing method comprises the following steps: step 1, performing cleaning; to be specific, removing residual fruit stems, leaves and non-medicinal substances; step 2, performing medicine washing; to be specific, pouring the medicinal materials into a stainless steel medicine washing pool or medicine washing machine, washing the medicinal materials with tap water to mainly remove surface impurities such as dust, washing the medicinal materials with water for about 2-3 minutes, and fishing out the medicinal materials with a leaking net; step 3, performing steaming; to be specific, putting the cleaned dried tangerine or orange peel medicinal materials into a medicine steaming machine, performing steaming for 30-120min, and performing moistening for 0-12h; and step 4, performing cutting; to be specific, cutting the steamed dried tangerine or orange peel medicinal materials and the like. According to the method, the dried tangerine or orange peel steaming technology is optimized through multiple indexes such as the hesperidin amount and the characteristic spectrum ofsteamed dried tangerine or orange peel is established; and the manufacturing process is quantified.

The invention discloses a method for separating synephrine from hesperidin waste liquid, which comprises the following steps: S1, loading a hesperidin mother liquid to strongly acidic cationic resin, after loading is finished, eluting with water, after water elution is finished, eluting with a strong alkali mixed liquid, collecting an eluate after elution with the strong alkali mixed liquid, and adjusting the pH value of the eluent to 5-7 to obtain a neutralized solution; and S2, carrying out ultrafiltration on the neutralized solution, then carrying out concentration through a nanofiltration membrane, and carrying out cooling crystallization, filtration and drying on the concentrated solution to obtain synephrine. According to the method for separating synephrine from the hesperidin waste liquid, the hesperidin waste liquid is reused, novel poncirin is extracted from the hesperidin waste liquid, the problem of sewage treatment is solved while economic benefits are obtained, and waste recycling is achieved. According to the method, ion exchange is carried out through the strongly acidic cationic resin, elution is carried out through the low-concentration strong alkali mixed solution, no strong pungent smell exists, the requirements for production equipment and the environment are low, and the requirement for green production is met.

The invention belongs to the technical field of traditional Chinese medicines, and specifically relates to application of a boenninghausenia sessilicarpa cough-asthma tablet to prepare medicaments for inhibiting lung cancer cell LLC proliferation and a preparation method for the boenninghausenia sessilicarpa cough-asthma tablet. The boenninghausenia sessilicarpa cough-asthma tablet provided by the invention possesses the application to prepare medicaments for inhibiting lung cancer cell LLC proliferation. The boenninghausenia sessilicarpa cough-asthma tablet is prepared from the following bulk drugs: 156 g of dried orange peel, 96 g of acorus gramineus, 312 g of reynoutria japonica, 188 g of radix asparagi, 375 g of boenninghausenia sessilicarpa, 96 g of stemona sessilifolia, 750 g of caulis marsdeniae tenacissimae, 375 g of laggera pterodonta, 96 g of semen armeniacae Amarum., 375 g of houttuynia cordata and 188 g of cortex mori. Supercritical extraction and microwave extraction are employed for preparing the boenninghausenia sessilicarpa cough-asthma tablet, and thus hesperidin content is substantially improved.

The invention discloses an EVA sockliner and a preparation process thereof. The sockliner comprises the following raw materials: 75-80 parts of EVA; 2-4 parts of a foaming agent; 1-2 parts of a cross-linking agent; 0.5-1 part of a release agent; 3-4 parts of an activator; 5-10 parts of orange peel powder; 0.8-1 part of silver nitrate; and 0.3-0.5 part of a coupling agent. Orange peel has natural exchange capability and absorption characteristic, and is often used for removing peculiar smell in daily life. The orange peel contains hesperidin and has a certain antibacterial effect. The silver nitrate has a good antibacterial effect; the solution of silver nitrate is acidic and has certain corrosivity and oxidability and can react with reducing substances in orange peel; hemicellulose in theorange peel powder is easily degraded under the acidic condition, and lignin is subjected to a condensation reaction under the acidic condition, such that the micro-pore channels of the orange peel powder are fine and uniform, and the adsorption capacity of the modified orange peel powder is improved. Silver ions are adsorbed to the surfaces of particles of the orange peel powder and in micro-pores, so that the silver ions can be endowed with a slow release effect and the sockliner can be endowed with a lasting and effective antibacterial effect.

The invention belongs to the field of pharmaceutical preparations, and discloses a method for improving the water solubility and bioavailability of hesperidin. A cyclodextrin embedding method is adopted, an alkalic system is used for solubilization, and the hesperidin is embedded in an inner cavity of cyclodextrin to prepare a hesperidin-cyclodextrin embedded object, so that the water solubility of the hesperidin is obviously improved, meanwhile the side reaction of hesperidin ring opening caused by the conventional technical means is avoided, the structural integrity of the hesperidin is ensured, and the oral bioavailability of the hesperidin is remarkably improved.

The invention provides a novel process method for extracting hesperidin. The novel process method comprises the following steps: crushing dried immature bitter oranges, carrying out pretreating with asodium citrate solution, carrying out extracting with an alkali liquor, adding acid for crystallization, and carrying out filtering and drying. The core of the application is the pretreatment processwith the sodium citrate solution; in the pretreatment process, a raw material can be washed; meanwhile, isomers of the hesperidin in part of raw material components can be removed. The concentrationof the sodium citrate solution is strictly controlled, so impurities in a hesperidin product can be effectively reduced, and the content of the hesperidin is increased. During crystallization, the pHvalue of an extracting solution is adjusted to be 4.5-6.5 by using the acid.

The invention discloses a method for preparing natural hesperidin through biological fermentation and extraction. Firstly, a fermentation culture solution is prepared, and is mixed with fresh tangerine peel or orange peel; then steam explosion treatment is performed, the material is inoculated with microorganisms and culture is performed, then parameters such as temperature and ventilatory capacity are adjusted for enzymolysis, crystallizing is performed after the enzymolysis is completed, and filtering is performed to obtain the natural hesperidin. The yield of the natural hesperidin can reach 42%. According to the method, the fresh tangerine peel or orange peel is directly used as the raw material, and operations of drying, crushing and the like are not needed, so that degradation of the hesperidin under the high-temperature condition in an extraction stage is avoided, the pollution of alkali liquor such as lime water to the environment is also avoided, the equipment requirement in the whole process is simple, the process flow is short, and the extraction yield of the hesperidin is high.