Nicotiana tabacum total alkaloids separation method in Nicotiana tabacum extract

A technology of total alkaloids and separation angle, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of residual resin monomer components, low transfer rate, and increased process steps, etc., to achieve reduction The process of regenerating the resin column, the effect of high alkaloid transfer rate and simple process flow

Inactive Publication Date: 2009-07-29
BEIJING HERUN INNOVATION PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above two methods have certain progress, there are still serious defects: in the test, it can be found that although the method can improve the purity of alkaloids, the transfer rate is still not high; It has better solubility in organic solvents such as ethanol, but the use of organic solvents will bring residues of resin monomer components such as styrene and d...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take 1000g Artemisia decoction pieces, add water to decoct twice, 10 times the amount each time, decoct for 2 hours, combine the decoction liquid, centrifuge, adjust pH=3, centrifuge, and the supernatant is passed through cation exchange at 4 times the column volume / hour Resin (001×7, hydrogen type, diameter-to-height ratio 1:8), washed with water until the effluent is colorless, then eluted with 15% hydrochloric acid solution, the elution rate is 2 times the column volume / hour, until the alkaloid test is negative (The effluent was added dropwise with 10% silicotungstic acid without precipitation), the eluate was collected, neutralized to neutral with sodium hydroxide, desalinated, and the filtrate was concentrated and dried to obtain the total alkaloids of Artemisia annua.

Embodiment 2

[0036] Get 1000g of Artemisia annua decoction pieces, add acidic water with pH=5 to percolate, combine percolate and add water to dilute to 10000ml, centrifuge, adjust pH=1, centrifuge, supernatant passes through cation exchange resin (Doulex 50, sodium type, diameter-to-height ratio 1:5), wash with water until the effluent is colorless, then elute with 5% hydrochloric acid solution, the elution rate is 4 times the column volume / hour, after elution is 2 times the column volume, soak for 5 hours , and then eluted until the alkaloid test was negative (10% silicotungstic acid was added dropwise to the effluent without precipitation), the eluate was collected, neutralized to neutral with calcium hydroxide, desalinated, and the filtrate was concentrated and dried to obtain the total organism of Artemisia horns alkali.

Embodiment 3

[0038] Take 5 kg of Artemisia annua decoction pieces, add 70% ethanol to ultrasonically extract twice, add 5 times the amount each time, ultrasonically for 0.5 hours, combine the extracts, centrifuge, recover ethanol, add water to 5000ml, adjust pH = 2, centrifuge, and the supernatant to 2 times the column volume / hour, pass through the upper cation exchange resin (001×10, sodium type, diameter-to-height ratio 1:7), wash with water until the effluent is colorless, and then wash with 1% hydrochloric acid solution (containing 1% sodium chloride) Elution, the elution rate is 5 times the column volume / hour, until the alkaloid test is negative (10% silicotungstic acid is added dropwise to the effluent without precipitation), the eluate is collected, neutralized to neutral with sodium hydroxide, and desalted , the filtrate was concentrated and dried to obtain the total alkaloids of Artemisia annua.

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PUM

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Abstract

The invention discloses a method for separating total alkaloid from traditional Chinese medicine, in particular a method for separating total alkaloid from an extracting solution of the traditional Chinese medicine, namely Incarvillea, and belongs to the field of traditional Chinese medicines. The method comprises the following technical proposal: taking the extracting solution of the Incarvillea; adjusting the pH value of the extracting solution to between 1 and 7; filtering the extracting solution; taking and adding the filtrate to a cation exchange resin column; rinsing the filtrate and removing the impurities; eluting the filtrate with 0.5 to 15 percent acid liquor; collecting eluent; adding alkali to the eluent for neutralization; performing desalting treatment on the mixed solution; and obtaining the Incarvillea total alkaloid. The method overcomes the defects of low extracting rate, large using level of organic solvent, complicated process, inability of large-scale production, and the like in the prior art and can efficiently separate high purity of the Incarvillea total alkaloid from the extracting solution of the Incarvillea.

Description

technical field [0001] The invention relates to the extraction and separation of active ingredients of traditional Chinese medicine, in particular to a method for extracting and separating total alkaloids of Artemisia annua from the extract liquid of Artemisia annua, which belongs to the field of traditional Chinese medicine. Background technique [0002] Alkaloids are a large class of basic nitrogen-containing compounds that widely exist in nature, are active ingredients of many Chinese herbal medicines, and are an important class of substances in nature. Artemisia japonicus is the dried whole herb of Incarvilla compacta Maxim., a plant of the Bignoniaceae plant. It is a commonly used folk Chinese herbal medicine, which has the functions of regulating menstruation and blood circulation, dispelling rheumatism, reducing inflammation and sharpening ears, and benefiting veins. Studies have shown that the total alkaloids of artemisinin is its main effective part, including seven...

Claims

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Application Information

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IPC IPC(8): A61K36/185
Inventor 朴美善王广录张峰
Owner BEIJING HERUN INNOVATION PHARMA TECH DEV
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