Nicotiana tabacum total alkaloids separation method in Nicotiana tabacum extract

Abstract

The invention discloses a method for separating total alkaloid from traditional Chinese medicine, in particular a method for separating total alkaloid from an extracting solution of the traditional Chinese medicine, namely Incarvillea, and belongs to the field of traditional Chinese medicines. The method comprises the following technical proposal: taking the extracting solution of the Incarvillea; adjusting the pH value of the extracting solution to between 1 and 7; filtering the extracting solution; taking and adding the filtrate to a cation exchange resin column; rinsing the filtrate and removing the impurities; eluting the filtrate with 0.5 to 15 percent acid liquor; collecting eluent; adding alkali to the eluent for neutralization; performing desalting treatment on the mixed solution; and obtaining the Incarvillea total alkaloid. The method overcomes the defects of low extracting rate, large using level of organic solvent, complicated process, inability of large-scale production, and the like in the prior art and can efficiently separate high purity of the Incarvillea total alkaloid from the extracting solution of the Incarvillea.

Description

technical field [0001] The invention relates to the extraction and separation of active ingredients of traditional Chinese medicine, in particular to a method for extracting and separating total alkaloids of Artemisia annua from the extract liquid of Artemisia annua, which belongs to the field of traditional Chinese medicine. Background technique [0002] Alkaloids are a large class of basic nitrogen-containing compounds that widely exist in nature, are active ingredients of many Chinese herbal medicines, and are an important class of substances in nature. Artemisia japonicus is the dried whole herb of Incarvilla compacta Maxim., a plant of the Bignoniaceae plant. It is a commonly used folk Chinese herbal medicine, which has the functions of regulating menstruation and blood circulation, dispelling rheumatism, reducing inflammation and sharpening ears, and benefiting veins. Studies have shown that the total alkaloids of artemisinin is its main effective part, including seven...

AI Technical Summary

Problems solved by technology

Although the above two methods have certain progress, there are still serious defects: in the test, it can be found that although the method can improve the purity of alkaloids, the transfer rate is still not high; It has better solubility in organic solvents such as ethanol, but the use of organic solvents will bring residues of resin monomer components such as styrene and divinylbenzene into the eluent, which will affect the safety of the preparation; at the same time, due to the use of Organic solvents also require an ethanol treatment step to be added to the pre-treatment of the resin, and the organic solvent must be recovered later, which increases the process steps, increases the production cost, and brings safety hazards to the final preparation