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Composition of cytokine and use thereof

A cytokine and composition technology, applied in the direction of drug combination, medical preparations containing active ingredients, microorganisms, etc., can solve the problem of single type of factor components, and achieve the effect of huge development potential and rich variety.

Inactive Publication Date: 2009-08-12
CHANGSHA HUI LIN LIFE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, most cytokine products are prepared by genetic engineering [15]-[18] , a single type of factor component

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] 1. Preparation of Feeder cells:

[0020] Human fibroblasts qualified for pathogenic testing were treated with mitomycin C in 3×10 4 / cm 2 Density inoculate 6-well plates at 37°C, 5% CO 2 After 24 hours to 72 hours, it is used to inoculate ES cells. The culture medium uses D / F-12 medium. Fibroblast cell lines were obtained according to general methods.

[0021] 2. Culture of ES cells:

[0022] ES cells qualified for pathogenic testing were obtained from human embryonic stem cell lines according to the method disclosed in literature 19-21, seeded on the Feeder at a density of 40-60 clone / well, 37°C, 5% CO 2 Culture, subculture once every 7 days, change the medium every 24 hours, collect the daily culture medium, store it at 4°C, and finally mix the collected liquid and store it at -80°C.

[0023] 3. Detection and storage of cytokine content in culture medium

[0024] Mix the liquids collected in 6 days, as a batch of products, take 0.5ml of the mixed liquid, use th...

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PUM

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Abstract

The invention relates to a composition of cellular factors. The invention also relates to medical application of the cellular factors. The composition of the cellular factors provided by the invention is stem cell culture fluid. The composition of the cellular factors has the advantages of having more diversified cellular factors and more natural cellular factor activity compared with the prior popular recombinant cellular factor preparation, and being a biological agent with huge development potential.

Description

technical field [0001] The present invention relates to a composition of cytokines. The present invention also relates to the medical use of the cytokine composition. Background technique [0002] Embryonic stem cells are a type of cells with self-renewal and multi-lineage differentiation capabilities, mainly derived from the inner cell mass of the blastocyst stage. At present, many human and mammalian embryonic stem cell lines have been established, and these stem cell lines are available to the public. For example, the US government has funded the establishment of a national-level stem cell bank at the University of Wisconsin, which stores 21 human embryonic stem cell lines registered by the NIH. Good human embryonic stem cell lines can be provided for researchers and non-profit institutions ( http: / / www.wicell.org / index.php?html option=com oscommerce&itemid=130 ). The conventional culture of embryonic stem cells also has a set of known operating procedures, and corres...

Claims

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Application Information

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IPC IPC(8): C12N5/06C12N5/08A61K38/18A61P17/02
Inventor 卢光琇林戈王建
Owner CHANGSHA HUI LIN LIFE TECH
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