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Achromobacter capable of degrading aniline and application thereof

A colorless bacillus, aniline technology, applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve problems that have not been discovered, and achieve enhanced impact resistance, high degradation efficiency and tolerance concentration, and good self- Effect of flocculation properties

Inactive Publication Date: 2011-04-13
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After retrieving patents and other relevant literature, no reports have been found on the use of Achromobacter to degrade aniline

Method used

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  • Achromobacter capable of degrading aniline and application thereof
  • Achromobacter capable of degrading aniline and application thereof
  • Achromobacter capable of degrading aniline and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1. Isolation, purification and identification of Achromobacter sp.adx-3

[0025] 1. Isolation and purification of Achromobacter sp.adx-3

[0026] Achromobacter sp.adx-3 is a Gram-negative bacterium obtained by sampling from the activated sludge in the aeration tank of Zhejiang Zhenhai Refining and Chemical Wastewater Treatment Plant, after domestication, isolation and purification. Specific steps are as follows:

[0027] Activated sludge from the aeration tank of Zhejiang Zhenhai Refining and Chemical Wastewater Treatment Plant was taken. Add 50mL sludge and 50mL acclimation culture solution (0.024g / L KH 2 PO 4 , 0.024g / L Na 2 HPO 4 , 0.0696g / LMgSO 4 ·7H 2 O, 0.022g / L CaCl 2 2H 2 O, 0.005g / L FeCl 3 , 0.00003g / L CuSO 4 ·H 2 O, 0.00013g / L MnSO 4 ·H 2 O, 0.00023g / L ZnCl 2 , 0.00042g / L CoCl 6H 2 O, 0.00015g / L NaMoO 4 2H 2 O, 0.00005g / L AlCl 3 ·6H 2 0), and 0.049mL aniline (500mg / L). Replace 50mL of culture medium every 5d, add 0.049mL of aniline...

Embodiment 2

[0041] Example 2. Achromobacter sp.adx-3 detection of aniline degradation performance

[0042] 1. Under the condition of using aniline as the only carbon and nitrogen source, investigate the degradation ability of adx-3 to different initial concentrations of aniline. It was found that when the initial concentration of aniline did not exceed 4000mg / L, adx-3 had a good removal effect on aniline. When the concentration of aniline reached 4500mg / L, the growth of the strain was inhibited, and the aniline degradation rate was about 25% within 120 hours. At the same time, with the increase of the initial concentration of aniline, the degradation inhibition period becomes longer, and the degradation rate of aniline in the degradation period becomes faster. Specific steps are as follows:

[0043] 1) Inoculate adx-3 from the solid medium into MM culture solution containing 500mg / L aniline, culture at 30°C, 180rpm / min on a shaker for 24h, and make it OD 600 =1.2±0.05.

[0044] 2) Add ...

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Abstract

The invention discloses an achromobacter capable of degrading aniline, wherein the 16S rDNA of the achromobacter has a sequence of SEQ ID No.1. Colonies are yellow, round, smooth and humid with neat edges and convex bulges. Through electron microscope observation, the achromobacter does not have flagellum and spore bacteria; and under the observation of the electron microscope, the form of the achromobacter is a thicker bacillus which is gram staining negative and oxidase and catalase negative, and is reduced by nitrate to generate gas. The achromobacter can achieve the efficient degradation of aniline pollutants under an aerobic condition, the maximum tolerance concentration to the aniline is up to 4,500 milligrams per liter, the aniline of which the concentration is less than or equal to 4,000 milligrams per liter can be completely removed, thus the achromobacter plays an important role in the controlling practice of aniline industrial wastewater.

Description

technical field [0001] The invention relates to the technical field of biodegradation, in particular to a novel aniline-degrading bacterium and its application. Background technique [0002] As a typical representative of chemically synthesized aromatic compounds, aniline is an important chemical raw material and fine chemical intermediate. There are more than 300 kinds of chemical products and intermediates made from it, which are widely used in pesticides, medicines, printing and dyeing, plastics and other industries. . At present, more than 80,000 tons of aniline are produced in China every year, and about 30,000 tons of aniline are discharged into the environment every year in the world. The aniline pollutants contained in the environment mainly come from the wastewater of pharmaceutical, rubber and many other chemical plants. [0003] Aniline is a highly toxic and high-residue substance that can cause damage to the central nervous system, cardiovascular system and oth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F101/38C12R1/025C02F3/34C12N1/20
Inventor 张丽丽陈建孟向正心姜理英
Owner ZHEJIANG UNIV OF TECH
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