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Killing method of colibacillus

A technology of Escherichia coli and low temperature plasma, applied in sanitary equipment, plasma, electrical components and other directions for toilets, can solve the problem of easy drug resistance, achieve the effect of low temperature, avoid degradation and denaturation

Inactive Publication Date: 2013-05-22
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sensitive to sulfonamides, streptomycin, chloramphenicol, etc., but easily resistant

Method used

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  • Killing method of colibacillus

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0021] First, draw 10 μl of freshly prepared E. coli suspension and spread it evenly on the treated PTFE slide, after drying, place it in the discharge reaction tube of the radio frequency plasma discharge device. PTFE is polytetrafluoroethylene, which is a common medical polymer material. It is often used in the manufacture of artificial hearts, artificial valves, artificial larynx, artificial blood vessels, and various medical catheters.

[0022] Then, adjust the discharge parameters of the radio frequency plasma discharge device as follows: the working gas is oxygen, the discharge power is 100W, and the gas flow rate is 60cm 3 / min, voltage 440V, current 105mA, background vacuum 7.0Pa; the sterilization effect after 30s is 3.5, and the sterilization rate is 99.97%; it tends to be stable after 80-90s, and the sterilization reaction is close to complete after 90s; at this time, The plasma temperature was up to 32°C. Among them, the sterilization effect is calculated by the f...

experiment example 2

[0024] First, draw 10 μl of freshly prepared E. coli suspension and spread it evenly on the treated PTFE slide, after drying, place it in the discharge reaction tube of the radio frequency plasma discharge device.

[0025] Then, adjust the discharge parameters of the radio frequency plasma discharge device as follows: the working gas is oxygen, the discharge power is 100W, and the gas flow rate is 60cm 3 / min, voltage 440V, current 105mA, background vacuum 7.0Pa; the sterilization effect after 60s is 3.9, and the sterilization rate is 99.99%; it tends to be stable after 80-90s, and the sterilization reaction is close to complete after 90s; at this time, The plasma temperature was up to 33°C.

experiment example 3

[0027] First, draw 10 μl of freshly prepared E. coli suspension and spread it evenly on the treated PTFE slide, after drying, place it in the discharge reaction tube of the radio frequency plasma discharge device.

[0028] Then, adjust the discharge parameters of the radio frequency plasma discharge device as follows: the working gas is oxygen, the discharge power is 100W, and the gas flow rate is 90cm 3 / min, voltage 440V, current 105mA, background vacuum 7.0Pa; the sterilizing effect was 4.1 in 90s, and the sterilizing rate was 99.99%; the sterilizing reaction was nearly complete after 90s; at this time, the highest plasma temperature was 37°C.

[0029] Experimental examples 1-3 illustrate that low-temperature oxygen plasma can effectively kill Escherichia coli in a short time.

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Abstract

The invention relates to the disinfection and sterilization field, in particular to a killing method of colibacillus. In the method, a carrier infected with colibacillus only needs to be placed in a low temperature plasma environment at the temperature of 10 DEG C to 50 DEG C and killed for 30 to 100 seconds, and the low temperature plasma environment is generated from a radio frequency plasma discharge device.

Description

technical field [0001] The invention relates to the field of disinfection and sterilization, in particular to a method for killing Escherichia coli. Background technique [0002] Escherichia coli (E.coli), commonly known as Escherichia coli, was discovered by Escherich in 1885. Escherichia coli is pathogenic to humans and animals, especially to infants and young animals (poultry), and often causes severe diarrhea and sepsis. It is a common prokaryote and is the normal flora in the intestinal tract of humans and most warm-blooded animals . According to different biological characteristics, pathogenic Escherichia coli can be divided into five categories: pathogenic Escherichia coli (EPEC), enterotoxigenic Escherichia coli (ETEC), enteroinvasive Escherichia coli (EIEC), enterohaemorrhagic Escherichia coli ( EHEC), enteroadhesive Escherichia coli (EAEC). [0003] Escherichia coli (E.coli) is a Gram-negative short bacillus that does not cause disease under normal living condit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/14H05H1/30
Inventor 陈杰瑢胡淼
Owner XI AN JIAOTONG UNIV