Method, kit and use for preparing pluripotent stem cells

Abstract

The invention provides a method for efficiently inducing pluripotent stem cells. Using lentiviral vectors to introduce six induction factors including Oct4 (POU5f1), Sox2, c-Myc, Klf4, UTF1, Rex1 (ZFP42) and p53 gene inhibitors into cells, can efficiently induce induced pluripotent stem cells.

Description

technical field The invention relates to a method, kit and application for inducing pluripotent stem cells. Background technique (1) Characteristics of stem cell pluripotency: Embryonic stem cells are called "pluripotent" stem cells because they have the ability to differentiate into three germ layer cells. In addition, embryonoma (EC) cells and embryonic germ (EG) cells have similar pluripotency to embryonic stem cells. The common characteristics of pluripotent stem cells are: large nucleoplasmic ratio, clone-like growth (human embryonic stem cells are relatively flat, while mouse embryonic stem cells are relatively raised, and the cell boundaries are not obvious), have AP enzyme activity, and specifically express SSEA4, TRA1- 60. Surface markers such as TRA1-81 (mouse embryonic stem cells also specifically express SSEA1, while human embryonic stem cells do not express SSEA1), and Oct4, Nanog, Sox2, rex1 (ZFP42), GDF3, lin28, mbd2, TEGF1 and other markers sex gene. In a...

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Problems solved by technology

However, without using c-Myc, the efficiency of inducing IPS cells will be greatly reduced, and it cannot be repeated every time, which proves that c-Myc still plays a very critical role in reprogramming

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