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Method for high-flux analysis of specimen containing biological marker and sample bank preparation

A biomarker, high-throughput technology, applied in the field of bioengineering, which can solve problems such as inability to detect fragment size

Active Publication Date: 2011-11-23
BIOCHAIN BEIJING SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0014] These array technologies can provide high-throughput screening methods for biomarkers, but they must be constructed with immovable media and affinity groups. Further, these technologies are not comparable to the traditional Northern blot and Western blot detection methods mentioned above. Subjective intent of binding, so indicators such as fragment size of mRNA or protein molecules cannot be detected

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  • Method for high-flux analysis of specimen containing biological marker and sample bank preparation
  • Method for high-flux analysis of specimen containing biological marker and sample bank preparation
  • Method for high-flux analysis of specimen containing biological marker and sample bank preparation

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Embodiment 1

[0189] This embodiment provides a high-throughput nucleic acid imprinting method (HT Northern blotting), and its preparation and analysis process includes the following steps:

[0190] 1) Isolation and purification of mRNA;

[0191] 2) mRNA agarose gel electrophoresis with RNA molecular weight standards;

[0192] 3) mRNA molecular weight calculation based on RNA molecular weight standard electrophoretic migration;

[0193] 4) The agarose gel with mRNA is cut into gel component strips;

[0194] 5) According to the primary setting sequence of the marker components such as the distance and order of each gel slice in the agarose gel, and according to the secondary setting sequence of the marker components of the mRNA source tissue;

[0195] 6) extracting and recovering componentized mRNA from corresponding gel strips;

[0196] 7) Spotting componentized mRNA on the corresponding support tissue according to the primary set sequence generated by the corresponding gel strip compone...

Embodiment 2

[0209] This embodiment provides a high-throughput Western Blot method (HT Western Blot). The principle of preparing high-throughput Western Blot is the same as that of high-throughput Northern Blot, although the method of componentizing and separating protein molecules is different. This embodiment provides a high-throughput nucleic acid imprinting method (HT Northern blotting), and its preparation and analysis process includes the following steps:

[0210] 1) Protein extraction and component separation;

[0211] 2) Protein polyacrylamide gel electrophoresis with protein molecular weight standards;

[0212] 3) Protein molecular weight calculation based on protein molecular weight standard electrophoretic migration;

[0213] 4) The polyacrylamide gel with protein is cut into gel component strips;

[0214] 5) According to the primary setting sequence of the marker components such as the distance and order of each gel slice in the polyacrylamide gel, etc., according to the sec...

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Abstract

The invention relates to a method for the high-flux analysis of a specimen containing a biological marker, a method for preparing a specimen bank of matrixing arrayed specimens for the high-flux analysis and a specimen bank prepared according to the methods. The invention combines the advantages of a molecular component technology with the high-flux advantages of the large-scale analysis of microarrays and giant arrays and provides a method for detecting valuable specimens economically and sensitively on the basis of a conventional molecular component technology.

Description

technical field [0001] The invention belongs to the technical field of bioengineering. Specifically, the present invention relates to a method for high-throughput analysis of samples containing biomarkers; the present invention also relates to a method for sample libraries of matrix-arranged samples for high-throughput analysis; the present invention also relates to The sample library prepared according to the method of the present invention. Background technique [0002] It is an important proposition in biomedical research to analyze biologically meaningful biomarkers derived from biological tissues in an appropriate form. Such biomarker molecules include but are not limited to: ribonucleic acid (RNA), deoxyribonucleic acid (DNA) or cDNA, protein, polypeptide, lipid, polysaccharide, usually required to be characterized by their corresponding biomedical properties, such as molecular weight, Charged for separation and componentization for more valuable and accurate analysi...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/53G01N33/558C40B50/14
Inventor 韩晓亮王建铭范盘生赵红
Owner BIOCHAIN BEIJING SCI & TECH
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