Preparation of immobilization acetylcholinesterase electrochemistry biosensor

A technology of acetylcholinesterase and biosensor, which is applied in the field of preparation of electrochemical biosensor to achieve the effect of high enzyme activity

Inactive Publication Date: 2009-10-07
UNIV OF SHANGHAI FOR SCI & TECH
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Problems solved by technology

At present, carbon nanotubes are used as electroactive materials and carriers of electroactive materials and have been widely used in electrochemical and biosensors, but they will be electrode

Method used

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  • Preparation of immobilization acetylcholinesterase electrochemistry biosensor
  • Preparation of immobilization acetylcholinesterase electrochemistry biosensor
  • Preparation of immobilization acetylcholinesterase electrochemistry biosensor

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Experimental program
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Embodiment

[0023] The present invention will be described in detail below in conjunction with the accompanying drawings.

[0024] The preparation steps of the immobilized acetylcholinesterase electrochemical biosensor are as follows:

[0025] (1) After the glassy carbon electrode is polished, it is pretreated by cyclic voltammetry scanning in pH7.40 phosphate buffer solution for 10 laps for electrochemical activation, and then it forms a two-electrode system with a platinum electrode for 2 hours to form a bright black band. Electrodeposited layer of carbon nanotubes with a large amount of negative charge.

[0026] (2) Immerse the electrodeposited glassy carbon electrode in step (1) to an ionic strength of 0.8mol L -1 Electrostatic self-assembly of polydiallyldimethylammonium chloride was carried out in a 1% polydiallyldimethylammonium chloride solution, and after 15 minutes, it was taken out and rinsed carefully with deionized water for 2 minutes, and then immersed in pH9 The concentra...

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Abstract

The invention relates to a method for preparing an electrochemistry biosensor, in particular an immobilization acetylcholinesterase method and a method for preparing current type biosensor. Specifically, electrodeposition technology is used to deposit a carbon nano tube with negative charge on the surface of glassy carbon electrode; subsequently, layer-by-layer electrostatic self-assembly process is conducted in polydiene propyl dimethyl ammonium chloride solution and carbon nano tube dispersion liquid to obtain a modified electrode with high self-assembly efficiency and stable modified layer. And then, layer-by-layer self-assembly is conducted again in the polydiene propyl dimethyl ammonium chloride solution and PBS of choline esterase to obtain an immobilization acetylcholinesterase current type biosensor with high stability. The method can quickly and stably detect the concentration of parathion in remains of pesticide under normal temperature.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical biosensor, in particular to a preparation method of an electrochemical biosensor with immobilized acetylcholinesterase. Background technique [0002] Organophosphorus pesticides play an irreplaceable role in world agricultural production. With the entry of WTO, the trade volume of agricultural products between my country and other countries has increased year by year, and importing countries have put forward higher and higher requirements for the quality of agricultural products. In order to improve the safety and quality of my country's agricultural products and enhance international competitiveness, the Chinese government attaches great importance to the problem of pesticide residues, vigorously develops the rapid detection technology of pesticides, and strengthens the real-time monitoring of agricultural products. Strengthening the detection and control of pesticide residues in agr...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 张淑平郑祎于大望丁亚平
Owner UNIV OF SHANGHAI FOR SCI & TECH
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