Low-molecular-weight chondroitin sulfate and preparation method thereof

Abstract

The invention discloses a low-molecular-weight chondroitin sulfate and a preparation method thereof. Macromolecular chondroitin sulfate is used as a raw material, and the low-molecular-weight chondroitin sulfate having average molecular weight of less than 1000 daltons is produced through a production process comprising chondroitin sulfate lyase degradation, deproteinization, filtration sterilization, drying, and the like. According to the low-molecular-weight chondroitin sulfate, the molecular weight distribution range is narrow, the content of chondroitin sulfate disaccharide is 43-60%, thecontent of chondroitin sulfate tetrasaccharide is 30-45%, the sum of contents of the chondroitin sulfate disaccharide and the chondroitin sulfate tetrasaccharide is greater than 87%, the total contentof oligosaccharides in the low-molecular-weight chondroitin sulfate is 97% or above, and the protein content is not more than 0.5%. Compared with common marketed macromolecular chondroitin sulfate, the product has a more obvious repairing effect on chondrocytes damaged by 1mM hydrogen peroxide at a concentration of 50-100 [mu]g / mL, has a strong repairing ability and a repair rate between 14% and23%, can be used to treat joint injuries, and is an important raw material for medical products, healthcare products, cosmetics, food, etc..

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a low molecular weight chondroitin sulfate and a preparation method thereof. Background technique [0002] Chondroitin sulfate (Chondroitin sulfate, CS, macromolecular chondroitin sulfate or chondroitin sulfate polysaccharide) is a kind of linear polysaccharide containing polyanions, composed of D-glucuronic acid (GlcA) and N-acetyl-D-amino semi Lactose (GalNAc) is connected by β-1,3 glycosidic bonds to form disaccharide units, and the disaccharide units are connected by β-1,4 glycosidic bonds, and sulfate groups are introduced at different positions during the subsequent biosynthesis process. Widely present in the cartilage and connective tissue of various animals. [0003] A large number of studies have shown that CS has the activities of lowering blood fat, anti-atherosclerosis, enhancing immunity, anti-viral hepatitis and anti-tumor. field. For exa...

AI Technical Summary

Problems solved by technology

[0009] The journal document "Enzymatic Preparation of Chondroitin Sulfate Oligosaccharides and Its Antioxidant Activity" Food Industry Science and Technology, 2017, 13, 48-52, discloses the use of chondroitin sulfate enzyme (molecular weight 76kDa) after fermentation from the enzyme-producing strain Acinetobacter sp.C26 ), the catalytic efficiency is low, the reported reaction concentration is only 2%, and the ratio of oligosaccharides has not been analyzed; the cracking has obtained chondroitin sulfate disaccharides and tetrasaccharides, and m / Z is 342 and 458 are disaccharides, m / What Z is 939 is a tetrasaccharide, which is different from our low molecular weight chondroitin sulfate in composition and molecular weight. For example, in our process, the molecular weight of disaccharide is about 379, 459 and the molecular weight of tetrasaccharide is about 838, 918, and the literature obtained The product has only been tested for antioxidant activity, and no other pharmacodynamic studies have been carried out

[0010] Therefore, domestic and foreign studies on the pharmacodynamics of low molecular weight chondroitin sulfate are not sufficient, especially for the main components of chondroitin sulfate disaccharide and chondroitin sulfate tetrasaccharide, and the content of disaccharide and tetrasaccharide is controlled within a certain range. The average molecular weight can be stably controlled below 1000 Daltons and its molecular weight distribution range is narrow. The low molecular weight chondroitin sulfate used to treat joint injuries needs further research and confirmation, so as to fill the research gap in this field at home and abroad.

Images