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Psoralea corylifolial extract with immunologic enhancement and application thereof

A technology of psoralen and extract, applied in the field of medicine, can solve the problems of no medicine available

Inactive Publication Date: 2009-10-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Psoralen contains a variety of active ingredients, and the glycosides represented by psoralen and isopsoralen are one of its important active ingredients. At present, there is no drug with total psoralen as the main ingredient.

Method used

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  • Psoralea corylifolial extract with immunologic enhancement and application thereof
  • Psoralea corylifolial extract with immunologic enhancement and application thereof
  • Psoralea corylifolial extract with immunologic enhancement and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 1 kg of psoraleae, dry at 50°C for 6 hours, add 10 times the amount of water and heat and reflux to extract twice, each time for 2 hours, filter the extract, concentrate under reduced pressure, alcohol precipitation, adjust the alcohol concentration to 70%, take The supernatant was concentrated to no alcohol and then extracted three times with ethyl acetate. The aqueous layer was separated, applied to macroporous resin, washed with water until sugar-free (molish reaction), discarded, and continued to elute with 70% aqueous ethanol. The eluate was collected, concentrated under reduced pressure until alcohol-free, and freeze-dried to obtain total psoralen glycosides. After liquid phase analysis, it contains two main components, the content is 40-50% respectively, see figure 1 , 1 in the figure is isopsoralen, and 2 is psoralen. Isopsoralen and psoralen will be separated, respectively do hydrogen spectrum, carbon spectrum, DEPT spectrum, see Figure 2-Figure 7 , it ...

Embodiment 2

[0022] Example 2 Effect of total psoralen glycosides on reduction of white blood cell count in normal mice caused by cyclophosphamide

[0023] 1. Purpose of the experiment: To investigate the effect of total psoralen glycosides on leukopenia induced by cyclophosphamide.

[0024] 2. Experimental materials

[0025] 2.1 Drugs and reagents: donkey-hide gelatin, Shandong Dong'e Ejiao Co., Ltd., batch number: 0311101; cyclophosphamide for injection (CTX), Jiangsu Hengrui Medicine Co., Ltd., batch number: 7063021; ​​white blood cell diluent: glacial acetic acid 2ml, 1% Gentian violet 1ml, add distilled water to 100ml.

[0026] 2.2 Experimental equipment: cell counting plate, microscope, 1000μl, 100μl micropipette.

[0027] 2.3 Experimental animals: ICR mice, male and female, weighing 18-22 g, provided by the Animal Center of Zhejiang Academy of Medical Sciences.

[0028] 3. Methods and steps

[0029] 3.1 Take healthy 18-22g ICR mice, and after feeding for 2 days under the conditi...

Embodiment 3

[0045] Example 3 The effect of psoralen on the reduction of white blood cell count in normal mice caused by cyclophosphamide

[0046] 1. Purpose of the experiment: To investigate the effect of psoralen on leukopenia induced by cyclophosphamide.

[0047] 2. Experimental materials

[0048] 2.1 Drugs and reagents

[0049] Ejiao, Shandong Dong'e Ejiao Co., Ltd., batch number: 0311101;

[0050] Cyclophosphamide for injection (CTX), Jiangsu Hengrui Medicine Co., Ltd., batch number: 07063021;

[0051] Leukocyte diluent: glacial acetic acid 2ml, 1% gentian violet 1ml, distilled water to 100ml.

[0052] 2.2 Experimental equipment: cell counting plate, microscope, 1000μl, 100μl micropipette.

[0053] 2.3 Experimental animals

[0054] ICR mice, half male and half female, weighing 18-22 g, were provided by the Animal Center of Zhejiang Academy of Medical Sciences.

[0055] 3. Methods and steps

[0056] 3.1 Take healthy 18-22g ICR mice, and after feeding for 2 days under the conditi...

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Abstract

The invention provides a psoralea corylifolial total glycoside extract which mainly contains 40 to 50 percent of psoralea corylifolial glycoside as well as 40 to 50 percent of isopsoralen glycoside. The psoralea corylifolial total glycoside extract and dressing or excipient allowed in pharmaceutics are prepared into any preparation formulation disclosed in pharmaceutics and can also be matched with other medicines or components to be prepared into a preparation together for use. Proved by pharmacological test, the psoralea corylifolial total glycoside and the psoralea corylifolial glycoside and the isopsoralen glycoside contained thereof can all resist the reduction of leucocyte caused by cyclophosphamide and have remarkable immunologic enhancement, and can be applied to the preparation of the medicines for increasing leucocyte.

Description

technical field [0001] The invention belongs to the field of medicine, in particular to a Chinese herbal medicine extract, mainly relating to the psoralen extract containing total psoralen glycosides and its application in the preparation of medicines for increasing white blood cells. Background technique [0002] At present, Chinese herbal medicine has a certain market all over the world. With the increase of people's awareness of health requirements and the aging of the population, sub-health status, people are more eager to return to nature, and use pure natural medicine with high degree of treatment, To prevent problems that cannot be solved by some chemically synthesized drugs, the application of natural herbal medicines is beyond the background of its original national traditional culture. It has become the goal pursued by pharmaceutical companies all over the world to seek medicines with low side effects and high quality and low price from natural medicines. The Euro...

Claims

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Application Information

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IPC IPC(8): A61K36/487A61P7/00A61P37/04A61K31/7048
Inventor 程翼宇瞿海斌吴斌
Owner ZHEJIANG UNIV
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