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Extraction and purification process for total flavonoids in Anchusa italica Retiz

A technology of total flavonoids and Buglossia, which is applied to plant raw materials, respiratory diseases, cardiovascular diseases, etc., can solve the problems of easily damaged medicinal ingredients, high cost of organic solvents, and large amount of solvents, so as to reduce the activity of extracts Loss, high safety, effect of improving extraction efficiency and yield

Inactive Publication Date: 2009-11-25
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the cold soaking method does not require heating, the extraction time is long and the efficiency is low
The percolation method has high extraction efficiency and less impurities in the immersion liquid, but it takes a long time, consumes a large amount of solvent, and is troublesome to operate
The reflux method is the most efficient, but it is easy to destroy the ingredients of the medicinal materials when heated, so it is not suitable to use this method
Other organic solvent extraction methods, this method has simple equipment and high product yield, but the impurity content in the product is high, and the cost of organic solvent is also high

Method used

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  • Extraction and purification process for total flavonoids in Anchusa italica Retiz
  • Extraction and purification process for total flavonoids in Anchusa italica Retiz
  • Extraction and purification process for total flavonoids in Anchusa italica Retiz

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Embodiment 1

[0027] The extraction method of a kind of Buglossia total flavonoids of embodiment 1 of the present invention

[0028] 1) Take 100 g of pulverized Buglossia, take 60% ethanol as solvent, soak for 12 hours, take 40% ethanol of 15 times the amount of medicinal material as extractant, heat with an electromagnetic oven with a power of 800W, and depressurize to 20Pa for reflux extraction 2 hours, collect the extract, filter, recover ethanol under reduced pressure, and concentrate to contain 22.5mg of extract per milliliter.

[0029] 2) Put the concentrated Buglossia extract on the column, control the flow rate of the effluent to 1BV / h, until the extract completely enters the resin bed, and let it stand for 1 hour to allow it to fully absorb. Rinse the resin bed with 2BV volume of deionized water, control the flow rate of the effluent to 2BV / h, to remove the part not adsorbed by the resin, elute with 3BV of 70% ethanol, the flow rate is 2BV / h, collect the eluate, and depressurize C...

Embodiment 2

[0030] The influence of embodiment 2 induction cooker operating power on total flavonoids extraction

[0031] Take three parts of pulverized Buglossia, 100g each, use 60% ethanol as solvent, soak for 12 hours, use 40% ethanol which is 15 times the amount of the medicinal material as extractant, and heat it with an induction cooker with a power of 500W, 800W and 1200W respectively , decompressed to 20Pa and refluxed for 2 hours, collected the extract, filtered, recovered ethanol under reduced pressure, concentrated to 22.5 mg of extract per milliliter, and used spectrophotometry to measure the absorbance to calculate the total flavonoid content, thus obtaining the total flavonoid absorption The amounts are 2.28g, 2.54g, and 2.56g, respectively. When the working power of the induction cooker is 800W and 1200W, the yield of total flavonoids is 2.54g and 2.56g respectively. For saving electricity, 800W is preferred.

Embodiment 3

[0032] The time of embodiment 3 decompression reflux extraction is on the influence of total flavonoids extraction

[0033] Get three parts of pulverized Buglossis, each part of 100g, take 60% ethanol as solvent, soak for 12 hours, take 40% ethanol of 15 times the amount of medicinal material as extraction agent, use a power of 800W for heating in an electromagnetic cooker, reduce pressure to Reflux extraction at 20 Pa for 1 hour, 2 hours, and 3 hours, collect the extract, filter, recover ethanol under reduced pressure, concentrate to 22.5 mg of extract per milliliter, and use spectrophotometry to measure the absorbance to calculate the total flavonoid content. The yields of flavonoids were 2.34g, 2.54g, and 2.60g, respectively. When the extraction time is 3 hours, the yield of total flavonoids is the highest. Considering the yield and saving, the optimal extraction time is 2 hours.

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Abstract

The invention relates to an extraction and purification process for total flavonoids in Anchusa italica Retiz, which comprises the following steps: 1) soaking crushed Anchusa italica Retiz in 60 percent ethanol taken as solvent for 12 hours, taking 30 to 50 percent ethanol of which the mass is 10 to 20 times of that of the medicament as an extracting agent, heating by an electromagnetic oven with the power between 500 and 1,200W, performing reflux extraction for 1 to 3 hours under reduced pressure less than 20Pa, collecting extracting solution, recovering the ethanol under reduced pressure, and obtaining concentrated solution; 2) purifying the extracted concentrated solution obtained in step 1) with macroporous resin, and eluting with ethanol solution; and 3) collecting the ethanol elution in step 2), condensing, performing freeze drying at vacuum, and obtaining a product. The process uses electromagnetic waves for heating, performs reflux extraction under reduced pressure and freeze drying, has little damage to active ingredients, and saves the extracting agent. Meanwhile, in the Anchusa italica Retiz extract product extracted by the method, the mass content of the total flavonoids is over 55 percent, the transfer rate of the total flavonoids is over 90 percent, and the repeatability is good because the resin can be recycled.

Description

Technical field: [0001] The invention relates to a method for extracting Uighur medicine, in particular to a method for extracting, purifying and drying the total flavonoids of Buglossia. Background technique: [0002] Bugloss is the above-ground part of Anchusa italice Retz. or Borage officinales L., a plant of Boraginaceae. During the peak flowering period in summer, the above-ground parts are generally harvested and dried in the sun. Slightly aromatic, light in taste and sticky, it is one of the common medicinal materials used by Uyghurs to treat cardiovascular and cerebrovascular diseases. Dryness and anti-inflammatory, cough and asthma and other effects. These effects of Buglossus are closely related to its flavonoids. Studies have shown that flavonoids have antibacterial, anticancer, antioxidative, antiviral, anti-inflammatory, antihypertensive and heat-clearing and detoxifying effects. Because of these biological activities of flavonoids, the research on it has en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/70A61P29/00A61P11/14A61P11/06A61P9/00
Inventor 秦大伟孟霞王世潇段洪东
Owner QILU UNIV OF TECH
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