Inactivated lactobacillus micro-ecological preparation and preparation method thereof

A technology of micro-ecological preparations and inactivated lactic acid bacteria, which is applied to non-active ingredient medical preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc., and can solve the problems of live bacteria death, increased risk of bacteriophage infection and outbreak, and colonization Poor ability and other issues

Active Publication Date: 2009-12-30
BIOFORTE BIOTECHNOLOGY (SHENZHEN) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the efficacy of probiotics has been widely proven and accepted, the following problems still exist in this type of live bacterial probiotics: (1) affected by environmental conditions (temperature, moisture, pH, etc.), its active ingredients (2) The high-temperature process of feed preparation and granulation kills a large number of living bacteria; (3) The antibiotics and chemically synthesized antibacterial agents added to the feed or used in the treatment process have a negative effect on the microecology. The preparation also has a killing or inhibiting effect; (4) cannot effectively resist the effects of hydrochloric acid or bile in the digestive tract of humans or animals, and has poor colonization ability in the intestinal tract
(5) Long-term use of the same live bacterial preparation in the same habitat increases the risk of phage infection and outbreaks
These problems greatly limit the application and promotion of live fungal probiotics, which is the bottleneck

Method used

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  • Inactivated lactobacillus micro-ecological preparation and preparation method thereof
  • Inactivated lactobacillus micro-ecological preparation and preparation method thereof
  • Inactivated lactobacillus micro-ecological preparation and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] An inactivated Lactobacillus acidophilus microecological preparation, its composition is that every kilogram of the inactivated Lactobacillus acidophilus probiotics contains: 550 grams of inactivated Lactobacillus acidophilus and its fermentation product, wherein the inactivated milk The bacillus cell content is 10 8 Each / g, specification is 80 mesh corn cob powder and precipitated silica 450 grams altogether, wherein corn cob powder and precipitated silica each 225 grams. The dosage in one ton of full price material is 2 kg. This component ratio and addition amount can be used in aquaculture.

Embodiment 2

[0030] The preparation method of the inactivated Lactobacillus acidophilus probiotics described in embodiment 1 may further comprise the steps:

[0031] 1) Lactobacillus acidophilus is first prepared for strains, and the strain screening medium is based on solid MRS medium as the basic medium, adding 0.1% CaCO 3 , the pH value is 6.4. Sterilize at 121°C for 20-30 minutes, cool to 37°C, and set aside.

[0032] 2) Fermentation culture of Lactobacillus acidophilus, the fermentation medium uses liquid MRS medium as the basic medium, and the pH value is 6.4. Sterilize at 121°C for 20-30 minutes, cool to 37°C, and set aside. The age of the seed bacteria is 12-18 hours, and the inoculation amount is 3%-5% (V / V or V / W). The fermentation time is 36-48 hours, the fermentation temperature is 37°C, and the pH value is maintained at about 5.5.

[0033] 3) Add 0.1-0.5% (w / v) protective agent to the fermentation broth after the fermentation, the protective agent is composed of sodium algin...

Embodiment 3

[0040] An inactivated Lactobacillus bulgaricus microecological preparation, its components are: 600 grams of inactivated Lactobacillus bulgaricus and its fermentation products per kilogram of inactivated Lactobacillus bulgaricus, wherein the inactivated Lactobacillus bulgaricus Cell content is 10 8 1 / g, 100 grams of glucose, 100 mesh corn cob powder and 300 grams of precipitated silica, wherein each 150 grams of corn cob powder and precipitated silica. The amount of one ton of full-price feed is 1 kg. This component ratio and addition amount can be used for piglet breeding. In the weaned piglet breeding experiment, adding 0.1% of this probiotic can reduce the diarrhea rate to 2.25% %, much lower than the 11.32% of the control group.

[0041] The glucose can be replaced by lactose.

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Abstract

The invention discloses an inactivated lactobacillus micro-ecological preparation and a preparation method thereof. The preparation comprises the following components in portion by mass: 10 to 60 portions of inactivated lactobacillus cell and lactobacillus fermentation product, and 30 to 50 portions of excipient, wherein the inactivated lactobacillus cell accounts for 10<8> to 10<9> cells per gram. The inactivated lactobacillus cell resists high temperature, gastric acid and bile, and has synergistic action with antibiotics; in addition, metabolic products produced in a lactobacillus fermentation process such as lactic acid, like bacteriocin and the like can effectively inhibit or kill enteric pathogenic bacteria of people or animals; and enzymes such as amylase, lactase and the like are helpful for digestion of the animals. The inactivated lactobacillus micro-ecological preparation effectively and economically solves the problem puzzling the prior viable bacteria micro-ecological preparation.

Description

technical field [0001] The invention relates to the field of probiotics for humans or feed, in particular to an inactivated lactic acid bacteria probiotics and a preparation method thereof. Background technique [0002] The rapid development of large-scale, high-density livestock and poultry and aquaculture has severely impacted the breeding ecological environment, resulting in frequent occurrence of highly infectious and fatal diseases. The use of traditional disinfectants and antibiotics can no longer effectively prevent the occurrence of diseases . As people pay more attention to food safety and personal health, they have realized the various disadvantages caused by long-term exposure to antibiotics or adding antibiotic additives in feed: (1) long-term use of antibiotics leads to the emergence of drug-resistant strains; Or the immune function of livestock, poultry and aquatic products is reduced. After antibiotics are ingested by the body, they will be distributed to va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61K47/36A23K1/16A23K1/00A61P11/00A61K35/744
Inventor 胡文锋毕英佐曹永长吴清平陈永泉
Owner BIOFORTE BIOTECHNOLOGY (SHENZHEN) CO LTD
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