Culturing method for tomato gynogenesis

A culture method and gynogenesis technology, which can be used in the field of further development and acquisition of plants, can solve problems such as death, ovary withering, and no callus obtained

Inactive Publication Date: 2010-03-03
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few studies on tomato gynogenesis. Ugur and Bal KazimAbak (2003) carried out tomato ovary culture, no callus was obtained, and the ovary gradually withered and finally died during the culture process.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culturing method for tomato gynogenesis
  • Culturing method for tomato gynogenesis
  • Culturing method for tomato gynogenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 callus induction culture

[0036] 1 Materials and methods

[0037] 1.1 Materials The test materials are two hybrid tomato varieties 'Zhongza 101' and 'Zhongza 105' bred by the Chinese Academy of Agricultural Sciences Institute of Vegetables and Flowers (purchased from the Chinese Academy of Agricultural Sciences Institute of Vegetables and Flowers), planted in the spring greenhouse from 2006 to 2008 , spring greenhouses, open fields, autumn greenhouses and autumn greenhouses to ensure annual sample collection. Each material is planted with 40-100 plants each time and managed according to conventional methods. One week after the plants are planted, suitable flower buds are selected for ovary and ovule separation. .

[0038] 1.2 Screening of induction medium A total of 20 kinds of induction medium were selected, the components of which were B5 and MS (both purchased from Sigma Company) as the basic medium, and different concentrations (1-100 μmol.L -1 ) aux...

Embodiment 2

[0066] Embodiment 2 differentiation culture and rooting culture

[0067] 1. Materials and methods

[0068] 1.1 Materials The experimental material is the callus obtained in Example 1. When the callus is formed and grows to about 2 mm, it is transferred to the differentiation medium under sterile conditions. Ovary, half ovary and ovule block also can adopt common culture method, be inoculated on the culture medium in the Erlenmeyer flask or culture dish.

[0069] 1.2 Screening of differentiation medium 60 kinds of differentiation medium were selected - B5, MS, DBM II (Greshoff P.M.Doy C.H.1972), Nitsch and N6 were used as basic medium, and different concentrations (0.1-1000μmol.L -1 ) auxins (indole acetic acid IAA, α-naphthalene acetic acid NAA, 2,4-D) and cytokinins (kinetin KT, 6-benzylaminopurine 6-BA, TDZ, zeatin ZT), the sucrose concentration was 2%, agarose concentration is 0.8%, pH5.8-6.0.

[0070] When the callus reached 2mm in size, it was transferred to the diffe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
Login to view more

Abstract

The invention provides a culturing method for inducing tomato gynogenesis. An excised ovule of a tomato bud in an E period or an F period is taken as an explant; and callus is induced and generated ona callus inductive medium, wherein the tomato bud in the E period or the F period is the tomato bud having encircled sepals and a length between 0 and 3 mm from the innermost of the crack of a sepalto the top of the petal. The culturing method for the tomato gynogenesis can obtain a large amount of callus, thereby providing a precondition for researching paths for inducing the gynogenesis to obtain a tomato haploid. Furthermore, the culturing method for the tomato gynogenesis differentiates the callus into a sprout on a specific culture medium and obtains a complete plant through rooting culture, which provides probability for researching the paths for inducing the gynogenesis to obtain the tomato haploid.

Description

technical field [0001] The invention relates to asexual reproduction technology, in particular to a method for inducing callus tissue from tomato gynonuclei and further developing and obtaining plants. Background technique [0002] The establishment of tomato haploid culture technology is of great significance for tomato breeding, molecular markers, genetic map construction, gene mapping, gene cloning, etc. Inducing androgenic and gynogenetic plants are two ways to obtain haploid. Induced androgenetic pathways include anther and microspore culture techniques, which have been applied to the breeding of barley, wheat, corn, rice, tobacco, flax, rapeseed, cabbage, eggplant, watermelon and other crops. The induced gynogenetic pathway involves unpollinated ovary and ovule culture and has been successful in crops such as wheat, rice, tobacco, garlic, sugar beet, and gerbera. The haploid culture of tomato began in 1971, and Sharp obtained the callus of cultivated tomato anthers. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 王孝宣
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap