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Physiological race identification method of tobacco phytophthora parasitica in disease nursery

A technology of Tobacco black shank and physiological race, which is applied in the field of plant pathogen identification, can solve problems such as tobacco black shank which has not been seen, and achieves the effect of good market application prospect.

Inactive Publication Date: 2010-03-17
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the identification method of the physiological race of tobacco black shank in the disease nursery, and there is no identification of the disease resistance of tobacco black shank for a certain specific physiological race in China

Method used

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  • Physiological race identification method of tobacco phytophthora parasitica in disease nursery
  • Physiological race identification method of tobacco phytophthora parasitica in disease nursery
  • Physiological race identification method of tobacco phytophthora parasitica in disease nursery

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Materials and methods

[0016] 1.1 Test materials

[0017] 1.1.1 Medium

[0018] Oat liquid medium was used for shaker enrichment culture, oat solid medium was used for strain activation and culture, and 0.05g / mL chloramphenicol was added for pathogen isolation and purification.

[0019] Sucrose-citric acid-vitamin-inorganic salt (SCBM) liquid medium: distilled water 1000mL, sucrose 30g, citric acid 20g, calcium chloride 1.0g, potassium nitrate 2.0g, potassium dihydrogen phosphate 0.67g, dipotassium hydrogen phosphate 0.33g , 10 drops of 1% ferric chloride, VB1 1mg. TTC liquid medium is 0.05% TTC added to the above medium; TTC solid medium is 0.05% TTC added to oat agar medium.

[0020] Table 1 Reagent specifications, purity and manufacturers used

[0021]

[0022] 1.1.2 Test varieties

[0023] N. plumbaginifolia, NC1071, L8, N. nudicaulis, Hongda (CK), K326 (CK), Pan Yuanhuang, BL-921, CV78-5.

[0024] 1.2 Method

[0025] 1.2.1 Identification of the host's...

Embodiment 2

[0056] Screening of germplasm resources resistant to tobacco black shank in this disease nursery

[0057] Tobacco black shank is a worldwide devastating fungal disease of tobacco caused by Phytophthora parasitica var.nicotianae (Breda deHean) Tucker. The pathogen belongs to the flagellate subphylum Phytophthora genus, is a facultative parasite that likes high temperature and high humidity, can harm all types of tobacco, and can infect roots, stems, and leaves at any stage of tobacco growth, causing wilting, stem damage and Plants die. In recent years, due to the expansion of the area of ​​continuous cropping tobacco fields and the increase of continuous cropping years, tobacco black shank has become more prevalent. In order to reduce the losses caused by the disease, people have used various methods such as agricultural control, chemical control, and disease-resistant breeding to control tobacco black shank disease. The post-growth period is short, the onset is fast, and mul...

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Abstract

A physiological race identification method of tobacco phytophthora parasitica in disease nursery can identify the physiological race of tobacco phytophthora parasitica in disease nursery as soon as possible by means of combining the identification of disease-resistant reaction of host in fields and collection of color change of strains on a TTC solid culture medium flat plate, realize the identification of the physiological race type of tobacco phytophthora parasitica in the existing disease nursery, then directionally carry out the researches such as tobacco disease-resistant germplasm resources screening, breeding of new variety of tobacco and the like, and provide basis for the comprehensive prevention and treatment of tobacco phytophthora parasitica and the establishment of the targetof breeding for disease resistance. The method is economical and practical and has favorable market application prospect.

Description

technical field [0001] The invention belongs to the technical field of identification of plant pathogens, and in particular relates to the identification of physiological races of tobacco blackleg pathogen and the identification of resistance to blackleg disease, and in particular to a method for identifying physiological races of tobacco blackleg pathogen. Background technique [0002] Tobacco black shank is one of the devastating diseases of tobacco (Nicotiana tabacum) production, and it is the most important factor restricting the production of tobacco leaves in various tobacco areas in my country. cultivated tobacco. Tobacco leaves lost due to tobacco black shank accounted for about 1% to 2% of the total output in North Carolina, USA. In 1995 and 1996, the losses caused by black shank reached 36.1 million US dollars, accounting for the total loss of the disease. 35.13%, ranking first among all diseases. The disease has a wide distribution in my country, except Heilongji...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12Q1/04C12R1/645
Inventor 李梅云李永平卢秀萍高家合刘勇
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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