Decoloration deodorization method of ocean activity collagen peptidase hydrolyzed liquor

A collagen and peptide enzymatic hydrolysate technology, which is applied in the general preparation and extraction fields of separation and purification of peptides, can solve the problems of unpleasant color and odor, complex color and fishy smell components, limited promotion and application, etc., and achieves mild treatment process. , easy operation, simple process effect

Inactive Publication Date: 2010-04-14
TAIXIANG GRP TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Marine active collagen peptides are mainly obtained by enzymatic hydrolysis, but in the process of preparation, due to the complex color and odor components of the enzymatic hydrolysis liquid, the color may contain newly formed components and fishy odor components in addition to the pigment components that have not been separated by centrifugation. It is mainly nitrogen-containing compounds such as amines, acids, carbonyl compounds, sulfur-containing compounds and other hydrocarbons, alcohols, phenols, aldehydes, etc. produced after substrate degradation, which causes an unpleasant The color and smell limit its promotion and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Weigh 1000ml of the prepared marine biological collagen peptide enzymatic hydrolysis solution, heat to 50°C, use 0.05mol / L NaOH solution to adjust the pH of the enzymatic hydrolysis solution so that the pH of the enzymatic hydrolysis solution is 5.5, and then add 5ml while stirring Powdered activated carbon is adsorbed at 50°C for 45 minutes for decolorization and deodorization; after decolorization and deodorization, use a disc centrifuge to centrifuge at 5000rpm for 40 minutes to remove the activated carbon in the enzymatic hydrolyzate of marine active collagen peptides. Just clear liquid.

Embodiment 2

[0010] Weigh 1000ml of the prepared marine biological collagen peptide enzymatic hydrolysis solution, heat it to 55°C, use 0.05mol / L NaOH solution to adjust the pH of the enzymatic hydrolysis solution so that the pH of the enzymatic hydrolysis solution is at 6, and then add 3ml while stirring Powdered activated carbon is adsorbed at 55°C for 45 minutes to decolorize and deodorize; after decolorization and deodorization, use a disc centrifuge to centrifuge at 5500rpm for 30min to remove the activated carbon in the enzymatic hydrolyzate of marine active collagen peptide. Just clear liquid.

Embodiment 3

[0012] Weigh 1000ml of the prepared marine biological collagen peptide enzymatic hydrolysis solution, heat to 52.5°C, use 0.05mol / L NaOH solution to adjust the pH of the enzymatic hydrolysis solution so that the pH of the enzymatic hydrolysis solution is at 5, and then add 6ml while stirring Powdered activated carbon is adsorbed at 52.5°C for 40 minutes for decolorization and deodorization; after decolorization and deodorization, use a disc centrifuge to centrifuge at 6000rpm for 20 minutes to remove the activated carbon in the enzymatic hydrolyzate of marine active collagen peptide, and take the Just clear liquid.

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Abstract

The invention relates to a decoloration deodorization method of ocean activity collagen peptidase hydrolyzed liquor, which comprises the following steps: heating the prepared ocean activity collagen peptidase hydrolyzed liquor up to 50 to 55 DEG C, using pH of 0.05 mol / L NaOH solution regulatory enzyme hydrolyzed liquor and leading the pH range to be between 5 and 6, then stirring up and at the same time charging volume 0.3 to 0.6% powder active carbon of enzymolysis liquor, absorbing for 40 to 45 minutes under the temperature of 50 to 55 DEG C, using a disc-type centrifugal machine after decoloring and deodorizing, centrifuging for 20 to 40 minutes at the speed of 5000 to 6000 rpm, removing active carbon and acquiring liquid supernatant. The method has the advantages of simple processes, easy operation, temperate treatment process, high safety and economic and practical performances. The enzymolysis liquor has better color and taste, the protein loss ratio is 4.2%, and the decoloration deodorization method is extensively applicable to industries such as health protection food and medicines.

Description

technical field [0001] The invention relates to a general preparation method for extracting, separating and purifying peptides, especially a method for decolorizing and deodorizing marine active collagen peptide enzymatic hydrolyzate. Background technique [0002] Marine active collagen peptides are rich in nutrients, easy to digest and absorb, have the functions of lowering blood pressure, lowering blood fat, anti-tumor, enhancing immune activity, activating cells, promoting skin metabolism and collagen synthesis, etc., and are widely used in food, cosmetics, medicine and health care products and other fields. Marine active collagen peptides are mainly obtained by enzymatic hydrolysis, but in the process of preparation, due to the complex color and odor components of the enzymatic hydrolysis liquid, the color may contain newly formed components and fishy odor components in addition to the pigment components that have not been separated by centrifugation. It is mainly nitro...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/14
Inventor郭玉华李钰金吴新颖陈绪乐殷诗强
OwnerTAIXIANG GRP TECH DEV