50 results about "Regulatory enzymes" patented technology

The present invention is directed to a method for localising, in chromatin or DNA, the binding loci of a chromatin or DNA binding protein comprising the following steps: a) tethering, directly or indirectly, an enzyme with regulatable activity to said chromatin binding protein, b) transiently activating the regulatable enzymatic activity bound to the targeted chromatin and c) mapping the enzymatically-modified genomic sites introduced by the enzyme into the chromatin. According to an embodiment of the invention, the regulatable enzyme is a regulatable protease. The mapping step may be carried out on specific DNA fragments, or on a chromosome-wide scale or a genome-wide scale. The present invention is also directed to kits for carrying out the methods of the invention.

The present invention belongs to the field of food-derived peptides and discloses a collagen peptide, a production method, a production device and an application thereof. The production method of thecollagen peptide comprises steps of coarse collagen solution heating, cooling, high-efficiency compound enzyme preparation enzymatic hydrolyzing, membrane filtering, concentrating, ultra-high pressurehomogenizing, and ultra-high temperature instantaneous sterilizing and drying. The high-efficiency compound enzyme preparation enzymatic hydrolyzing uses a compound enzyme preparation comprising compound protease, alkaline protease, neutral protease, flavor protease, animal proteolytic enzyme and an enzyme activator, and the enzyme activator is dithiothreitol and / or beta-mercaptoethanol. The provided method does not need to adjust pH value of the enzymatic hydrolyzing, does not need to control enzymatic hydrolyzing temperatures, is short in enzymatic hydrolyzing time and small in enzyme addition amount, and enables the enzymatic hydrolyzed product to be low and concentrated in molecular weight. The prepared collagen peptide powder is free of fishy smell, extremely excellent in water resolubility and also not liable to absorb moisture and agglomerate during storage process, and can be used in solid beverages, effervescent tablets, chewable tablets, health-care foods and feature-beautifying and skin-protecting products.

A method for preparing peanut antioxidant peptide through ultrasonic-assisted enzymolysis comprises the following steps: adding petroleum ether to a peanut cake powder, performing digestion and degreasing at a constant temperature, and performing drying to obtain a degreased peanut cake powder; adding distilled water to the degreased peanut cake powder, performing ultrasonic dissolution, and obtaining a peanut cake powder suspension; adjusting the pH value of the peanut cake powder suspension through a hydrochloric acid, adding composite plant hydrolase ViscozymeL, performing ultrasonic-assisted enzymolysis, obtaining enzymolysis liquid, deactivating enzyme, and performing cooling to the room temperature; adjusting the pH value of the enzymolysis liquid with a sodium hydroxide solution, adding protease, performing ultrasonic-assisted enzymolysis, deactivating enzyme, performing cooling to the room temperature, performing centrifugation, and performing freezing and drying to obtain peanut antioxidant peptide.

The invention relates to a decoloration deodorization method of ocean activity collagen peptidase hydrolyzed liquor, which comprises the following steps: heating the prepared ocean activity collagen peptidase hydrolyzed liquor up to 50 to 55 DEG C, using pH of 0.05 mol / L NaOH solution regulatory enzyme hydrolyzed liquor and leading the pH range to be between 5 and 6, then stirring up and at the same time charging volume 0.3 to 0.6% powder active carbon of enzymolysis liquor, absorbing for 40 to 45 minutes under the temperature of 50 to 55 DEG C, using a disc-type centrifugal machine after decoloring and deodorizing, centrifuging for 20 to 40 minutes at the speed of 5000 to 6000 rpm, removing active carbon and acquiring liquid supernatant. The method has the advantages of simple processes, easy operation, temperate treatment process, high safety and economic and practical performances. The enzymolysis liquor has better color and taste, the protein loss ratio is 4.2%, and the decoloration deodorization method is extensively applicable to industries such as health protection food and medicines.

The present invention relates to methods for modulating the migratory activity of cells expressing CD38 for the treatment of disorders including, but not limited to, inflammation, ischemia, asthma, autoimmune disease, diabetes, arthritis, allergies, infection with pathogenic organisms and transplant rejection. Such cells include, for example, neutrophils, lymphocytes, eosinophils, macrophages and dentritic cells. The invention further relates to drug screening assays designed to identify compounds that modulate the ADP-ribosyl cyclase activity of CD38 and the use of such compounds in the treatment of disorders involving CD38 modulated cell migration. The invention is based on the discovery that CD38 ADP-ribosyl cyclase activity is required for chemotaxis. Furthermore, the invention relates to methods for identifying compounds that modulate the enzyme activity of the S. mansoni CD38 homologue and using those compounds in the treatment of pathologic disorders caused by helminth infection. This is based on the discovery that helminths such as S. mansoni express CD38 homologues.

The invention belongs to the field of abalone peptides, and discloses an abalone peptide and a production method thereof. The production method of the abalone peptide comprises the following steps: pretreating abalones, carrying out enzymolysis by using a high-efficiency compound enzyme preparation under the conditions that the pH value is not adjusted and the temperature is not controlled, filtering by using an inorganic nanofiltration ceramic membrane to remove components which are difficult to dissolve and easy to absorb moisture, carrying out high-temperature high-pressure treatment on a peptide solution, concentrating, and drying to prepare powder. By adopting the method provided by the invention, the pH value of enzymolysis does not need to be adjusted, the enzymolysis temperature does not need to be controlled, the enzymolysis time is short, the enzyme addition amount is small, the molecular weight of an enzymolysis product is low and concentrated, and the prepared abalone peptide powder is excellent in water re-solubility and not prone to moisture absorption and caking in a storage process.

The invention belongs to the field of oyster peptide and discloses oyster peptide and a production method thereof. The production method of the oyster peptide comprises the following steps: pretreating oysters, performing enzymolysis with a high-efficiency compound enzyme preparation under the conditions that pH value is not adjusted and the temperature is not controlled, performing filtration with an inorganic nanofiltration ceramic membrane to remove insoluble and moisture-absorbing components, and performing high-temperature and high-pressure treatment, concentrating, drying for powder preparation. The method requires no adjustment of enzymolysis pH value or control of enzymolysis temperature, and has the advantages of short enzymolysis time, small enzyme addition quantity and low and concentrated molecular weight of enzymolysis products; prepared oyster peptide powder has excellent water solubility and is not prone to moisture adsorption and caking.

The present invention provides a method for simultaneously determining the DL-ATC content, the L-cysteine content and the L-cystine content in an enzyme catalysis reaction solution. The method comprises: adjusting the pH value of an enzyme catalysis reaction solution, and separating and simultaneously determining the DL-ATC content, the L-cysteine content and the L-cystine content in the enzyme catalysis reaction solution by using a HPLC method. According to the present invention, by adjusting the pH value of the enzyme catalysis reaction solution, the DL-ATC concentration, the L-cysteine concentration and the L-cystine concentration in the enzyme catalysis reaction solution can maintain the stable states within a certain time, and the rapid and simultaneous determination of the DL-ATC content, the L-cysteine content and the L-cystine content in the enzyme catalysis reaction solution by using the high performance liquid chromatography method can be achieved.

The invention belongs to the field of pearl shell peptide and discloses a pearl shell peptide as well as a production method thereof. The production method of the pearl shell peptide comprises the following steps: performing pretreatment on raw materials for pearl shell, performing enzymolysis for a high efficiency compound enzyme preparation under the conditions of no adjusting of pH value or controlling of temperature, performing filtering to remove insoluble and hygroscopic ingredients through an inorganic nanofiltration ceramic film, and performing high temperature and high pressure treatment on a peptide solution, and performing concentration, drying and powder production. By use of the production method, no adjustment of the pH value of enzymolysis or control of the temperature of enzymolysis is required, the enzymolysis time is short, the adding quantity of enzyme is small, and molecular weight of enzymolysis products is low and concentrated. The prepared pearl shell peptide powder has excellent water resolubility, and is not prone to moisture absorption or caking during storage.

A method of identifying a lead or candidate compound that modulates the activity of GTPase-Activating Protein SH3 Domain-Binding Proteins (G3BP) is provided, which includes determining whether a compound modulates the interaction between the N-terminal Nuclear Transport Factor 2-like (NTF2L) domain of G3BP and FGDF peptide of ubiquitin specific protease 10 (USP10) or non-structural protein 3 (nsP3).

The invention mainly relates to the technical field of silk processing, and discloses a treatment method of silkworm cocoon degumming wastewater. The treatment method comprises the following steps: carrying out potential of hydrogen regulation, carrying out enzymolysis, carrying out primary fermentation, carrying out secondary fermentation and discharging; the method is simple and convenient to operate, does not need special instruments and equipment, and is convenient to popularize; a removal rate of protein in the degumming wastewater reaches 97.3 percent, and a fat removal rate reaches 98.1 percent; macromolecular nutrients in the degumming wastewater are remarkably reduced, the environment is protected, and energy sources are solved; the degumming wastewater contains a large quantity of acid or alkali; after the potential of hydrogen regulation is carried out, the acid-alkali irritation of the degumming wastewater is reduced, the post-period treatment is facilitated, and the treatment effect is improved; after the potential of hydrogen is regulated, a complex enzyme is added and is used for carrying out the enzymolysis to decompose macromolecular fat and pectin components in the wastewater; then proteinase is added to decompose a large quantity of sericin protein in the wastewater, so that a condition that a large quantity of dissolved oxygen is consumed by oxidization and decomposition of fat and protein after the wastewater is discharged is avoided, and environment and water body pollution is alleviated.

The present invention provides methods to modulate key elements along the DAG signaling pathway as well as a diagnostic assay, device and methods of using the same to diagnose bipolar disorder (BD) and attention deficit hyperactivity disorder (ADHD). Methods to identify diagnostic markers and drug targets for BD and ADHD. Methods of identifying effective compounds responsible for membrane potentials and excitabilities influencing bipolar disorder (BD) and attention deficit hyperactivity disorder (ADHD). Methods of identifying an effective compound that modulates the activity of Ca2+ / CaM enzyme and compounds involved in changing the K+ gradient across the plasma membrane thereby increasing or decreasing the membrane potential ratio (MPR™) values. The invention provides methods of identifying a compound that modulates the activity of PKC which is an important protein of the DAG signaling pathway. Methods of identifying a compound that modulates DAG and its related enzymes along the DAG signaling pathway are provided. These compounds decrease or increase the membrane potential ratio (MPR™) in BD and ADHD patients.

The present invention relates to a method for increasing the selectivity of an enzyme, which method comprises performing the reaction in the presence of an enhancing compound selected from one of ethylene glycol, propylene glycol or glycerol.

The invention discloses a method for peeling a persimmon and the application of the peeled persimmon. The method for peeling a persimmon comprises the following steps: preparing a buffer solution and an enzyme solution → adjusting the pH of the enzyme solution to 4.0-4.5, selecting and cleaning raw materials for the persimmon →Remove the waxy layer→Immerse the waxy persimmons in the enzyme solution→Soak at a constant temperature of 40‑50°C for 1.5‑2h→Remove the enzymatically hydrolyzed persimmon skin→Wash the persimmons→Peel the skin and make persimmon cakes, dried persimmons, and persimmon wine Product; the step of removing the waxy layer in this method is to use the brush tumbler to remove the outermost gelatinous layer of the persimmon, and then carry out the enzymatic hydrolysis of the persimmon, which is an effective method proposed for the characteristics of the persimmon itself. A breakthrough in peeling. Compared with mechanical peeling and manual peeling, the brush tumbling machine can maintain a uniform force to remove the waxy layer on the outer surface of the persimmon, which can ensure the integrity of the remaining part of the persimmon without damaging the pulp. 2. Prevent the persimmon pulp from being oxidized when exposed to the air, and the nutritional flavor of persimmon can be better preserved.

The invention relates to the technical field of high-value utilization of marine biological resources, and discloses a high-activity marine biological peptide prepared by an enzymolysis method and application of the high-activity marine biological peptide in food. The target high-activity marine biological peptide and an affinity molecular switch sensitive to the concentration change of the target high-activity marine biological peptide are jointly fixed on a magnetic nano-carrier with bitter peptide adsorption functional groups on the surface to obtain the intelligent enzyme carrier; and adding the intelligent enzyme carrier into a reaction system containing a marine protein raw material and a first amphiphilic microcapsule wall material to carry out an enzymatic hydrolysis reaction in the reaction process. By constructing an intelligent enzyme carrier integrated with a directional enzyme and an affinity molecular switch, precise cutting and process self-control of an enzymolysis reaction are realized, and the affinity molecular switch can respond to the change of the concentration of the high-activity marine biological peptide and spontaneously adjust the enzyme activity, so that excessive degradation of a target product is effectively avoided.

Colorectal cancer (CRC) is one of the leading causes of cancer-related mortality in men and women. Uncontrolled proliferation of CRC cells is pivotal in colorectal tumorigenesis and cyclooxygenase-2 (COX-2) is an important regulatory enzyme in this process. Timely diagnosis is the key to life-saving management of CRC, which is under-diagnosed because colorectal aberrant crypt foci, hyper plastic polyps, micro edemas are often missed with conventional colonoscopy. Disclosed herein are imaging agents tagged to nanobodies specific for COX-2 protein that can selectively accumulate and retain in COX-2 expressing cells and colorectal adenomas, resulting in specific and sensitive of early detection, while reducing background and off-target systemic toxicity. The prospect of combining structural and functional imaging readouts obtained with fluorescence-aided colonoscopy measurements would provide powerful diagnostic information for improved management of colorectal carcinogenesis.

A method of characterizing a protein is provided herein. The method includes accessing simulated protein structure data with a computer system, where the simulated protein structure data indicate a structure of the protein. The method further includes quantifying, using the computer system and based on the simulated protein structure data, a plurality of dynamics metrics for a plurality of residues in the protein. The plurality of dynamics metrics are related to functional behaviors of the protein using the computer system. Additionally, the method includes generating a report from the functional behaviors and the dynamics metrics using the computer system, where the report comprises a functional characterization of each residue in the plurality of residues and a functional characterization of the protein.

The invention provides application of yellow chia seed oil to preparation of anti-fatigue medicine, healthcare products or food. According to research findings, the yellow chia seed oil acts on exhausted swimming laboratory mice, has the obvious inhibiting effect on lactic acid, urea nitrogen, creatine kinase and the like, has a certain effect of adjusting regulatory enzyme such as pyruvate kinase, malate dehydrogenase, succinate dehydrogenase and phosphofructokinase of related energy metabolism in muscle tissue, is good in safety and can be applied to the food, the healthcare products or the medicine with the anti-fatigue effect.

The invention provides a mechanism for improving the cold resistance of peach fruits through an IAA16 gene and application of the mechanism. The invention discovers that the IAA16 gene can improve the cold resistance of peach fruits in low-temperature refrigeration by simultaneously regulating an enzyme system and a non-enzyme system through treating peach fruits with exogenous beta-ionone. The regulatory enzyme system improves the ability to remove active oxygen by improving the activity of SOD, CAT and POD. The adjustment of the non-enzyme system comprises the steps of improving the contents of AsA, GSH and phenolic substances to directly neutralize active oxygen and adjusting the efficiency of cyclic regeneration of AsA and GSH by AsA-GSH to remove the active oxygen. The invention provides a theoretical basis for improving the development of a fruit fresh-keeping strategy by a fresh-keeping technology, and provides a novel and feasible technology for fruit fresh-keeping.

The invention discloses application of N-acetyl glucosamine as a marker in preparation of a reagent for diagnosing ischemic cerebral apoplexy. According to the invention, differential expression of N-acetyl glucosamine in serum of ischemic cerebral apoplexy and non-ischemic cerebral apoplexy contrasts is shown, the N-acetyl glucosamine is taken as a marker to quantitatively detect the metabolic level in the serum, and meanwhile, the N-acetyl glucosamine can be combined with a regulatory enzyme N-acetyl glucosamine kinase to relieve brain injury. Therefore, the N-acetyl glucosamine metabolism level detection has a very high clinical application value in the diagnosis of the ischemic cerebral apoplexy, and a brand new way is provided for the rapid and effective diagnosis of the ischemic cerebral apoplexy.

The invention discloses a method for reducing the content of an M impurity in fusidic acid fermentation liquor. The generation of the M impurity is inhibited from a fermentation source by optimizing a fermentation culture medium and regulating and controlling pH in stages. A fermentation culture medium adopts a composite organic nitrogen source, contains soybean cake powder with specific grease content, and is matched with a yeast extract and corn steep liquor, so that a key precursor can be provided for fusidic acid synthesis, and the enzyme activity can be regulated; the pH is regulated in the early stage of fermentation to adapt to the proliferation requirement of the strain, M impurity synthesis is preliminarily inhibited, the pH is regulated in the middle and later stages to strengthen the activity of a target product synthetase, and M impurity synthetase expression is remarkably inhibited. The process is simple, subsequent complex purification is not needed, the yield of fusidic acid is increased, M impurities are reduced to 2% or below, and the method is suitable for industrial large-scale production.

The invention belongs to the field of scallop peptide and discloses a scallop peptide and a production method thereof. The production method of the scallop peptide comprises the steps that scallops are subjected to raw material pretreatment, a high-efficiency compound enzyme preparation is subjected to enzymolysis under the condition that the pH value is not adjusted and the temperature conditionis not controlled, insoluble and easy-moisture-absorption components are removed through filtering of an inorganic nanofiltration ceramic membrane, high-temperature and high-pressure treatment and concentration are conducted, and powder is prepared through drying. By adopting the method, the pH value of enzymolysis does not need to be adjusted, the enzymolysis temperature does not need to be controlled, the enzymolysis time is short, the application amount of an enzyme is low, the molecular weight of an enzymolysis product is low and centralized, prepared scallop peptide powder is good in secondary dissolubility in water, and moisture absorption and caking do not easily occur in the storage process.

The invention belongs to the technical field of biological medicines, and particularly relates to application of neophytadiene in preparation of a medicine for preventing and treating premature senility of lung cells. According to the invention, the biological character change, the apparent transcriptional regulation effect and the internal molecular mechanism of the premature senility of the lung cells induced by the nano polystyrene are studied. Wherein the exposure of the nano polystyrene can induce the increase of cell premature senility phenotype, SA-beta-gal activity, ROS and SASP levels, and S-phase retardation of a cell cycle, and can activate the methylation modification of m6A and m7G of lung cell RNA, up-regulate the expression of RNA methyltransferase, promote the combination of METTL1 and a premature senility core gene, and accelerate the premature senility of cells. The neophytadiene NPT intervention can remarkably reverse ROS and SASP levels in cells, effectively relieve the cell cycle arrest degree, reverse the expression abnormity of RNA methylation regulatory enzyme, delay the premature senility process and the like. The invention provides a new thought and direction for prevention and intervention measures of nano-plastic induced lung injury.

The invention relates to the technical field of bioactive substance extraction, and discloses a safflower polypeptide extraction method and application, the safflower polypeptide extraction method comprises the following steps: S1, raw material pretreatment: crushing safflower seed kernels, mixing the crushed safflower seed kernels with an alkaline solution for extraction, and carrying out centrifugal separation to obtain a protein extract; s2, performing multi-stage enzymolysis: S201, adding first compound protease into the protein extracting solution, and performing first-stage enzymolysis; s202, adding second compound protease to carry out second-stage enzymolysis; s3, enzyme deactivation and purification: S301, adjusting the pH value of the enzymatic hydrolysate to an acidic condition to inactivate the enzyme; s302, separating the target polypeptide through a multi-stage ultrafiltration membrane; s303, drying to obtain a finished product of the safflower polypeptide. By controlling the adding sequence and reaction conditions of the alkaline protease, the trypsin and the flavourzyme in stages, the sequence design avoids competitive inhibition of a multi-enzyme system, so that the yield of a target peptide fragment is increased by 40% or above compared with that of a single-enzyme method.

The invention discloses a compound enzyme-assisted phlorizin extraction method, and particularly relates to the technical field of phlorizin extraction.The method comprises the following steps that 1, plant raw materials rich in phlorizin are selected and pretreated; 2, weighing the raw material powder, adding the raw material powder into an enzymolysis reaction kettle, and adding a citric acid-sodium citrate buffer solution; 3, cellulase, pectinase and papain are weighed and compounded into an enzymolysis system, and the enzymolysis system is injected into an enzymolysis reaction kettle; 4, the temperature in the enzymolysis reaction kettle is adjusted in real time, and an ultrasonic generator is started to work; 5, discharging the reaction liquid into a horizontal spiral centrifuge, and collecting supernate; and 6, extracting the precipitate generated in the step 5 for multiple times by using a proper amount of ethanol, and combining the extract and the supernatant. The method breaks through the limitation of a traditional process, under the mild condition, the phlorizin yield is increased by 35%, the thermal degradation rate is smaller than 2%, the impurity removal rate is larger than 90%, meanwhile, the extraction time is shortened by 66%, and the comprehensive cost is reduced by 40%.

The invention discloses a beta-galactosidase mutant E935V with enhanced activity and regulated and controlled by a salt solution and application of the beta-galactosidase mutant E935V. The amino acid sequence of the mutant is shown as SEQ ID NO. 1. When the salt is not added, the hydrolytic activity of p-nitrophenyl-beta-D-galactopyranoside (pNPG) of E935V is only 25.34% of that of the recombinant wild enzyme, while the activity of the mutant E935V in a salt adding system is obviously improved, and the maximum specific enzyme activity after improvement reaches 77.97 U / mg and is improved by 22.88% compared with that of the recombinant wild enzyme when the salt is not added. In 200 mM of KBr, the hydrolytic activity of the pNPG is improved by 3.9 times, and the hydrolytic activity of the pNPG on lactose is improved by 3.6 times. The mutant is suitable for a technical application scene in which the enzymatic reaction rate needs to be adjusted, the reaction efficiency and controllability are improved, and a better solution is provided for enzyme catalysis application in the fields of agriculture, food processing, biotechnology and the like.

The invention provides a method for improving protoplast conversion efficiency or gene editing efficiency of fungi and a kit. The method comprises the following steps: adding TritonX-100 or an analogue thereof, a bacteriostatic agent for inhibiting mitosis, or inositol or an analogue thereof in or before a protoplast transformation process taking gene editing as a target; more preferably, by adjusting the concentration of the sgRNA-Cas enzyme, the protoplast conversion efficiency and gene editing efficiency of fungi are effectively improved, the time for obtaining transformants or gene editing products is greatly saved, and the obtaining amount is increased.

The invention discloses a collagen peptide with effects of soothing nerves and helping sleep as well as a preparation method and application of the collagen peptide, and belongs to the field of collagen peptides. Comprising the following steps: (1) raw material pretreatment: mixing animal skin or cartilage or fish scales with water, and carrying out high-temperature moistening and swelling treatment; (2) alkali treatment: adjusting the pH value of the moistened and expanded system to 9.8-10.2; (3) directional enzymolysis: under the conditions that the temperature is 53-57 DEG C and the pH value is 9.8-10.2, firstly adding alkaline protease to carry out first-stage enzymolysis, and then adding neutral protease to carry out second-stage enzymolysis; (4) acid treatment and inactivation: adjusting the pH value of the system after enzymolysis to 4.8-5.2, and then heating to 88-92 DEG C for enzyme deactivation; (5) neutralization: adjusting the pH value of the system after enzyme deactivation to 5.8-6.2; (6) adsorption decoloration and filtration: adding activated carbon for adsorption, and then filtering to obtain a primary clarified solution; (7) membrane separation: performing nanofiltration membrane filtration on the primary clarified liquid to obtain concentrated peptide liquid; and (8) sterilization: carrying out ultra-high temperature instantaneous sterilization on the concentrated peptide liquid.

The invention provides a method for promoting conversion of food waste into reducing sugar, which comprises the following specific steps: crushing food waste, extracting oil and pulping to obtain high-concentration food waste thick slurry; the preparation method comprises the following steps: directly mixing choline chloride and glycerol without adding water to prepare a deep eutectic solvent; uniformly mixing the thick food waste slurry with the deep eutectic solvent, and performing ultrasonic centrifugation to collect solid-phase precipitates of the food waste; the method comprises the following steps: inoculating aspergillus into a container containing an enzyme-producing culture medium, culturing, extracting crude enzyme liquid, and adding protease to obtain a mixed enzyme preparation; adding water into the solid-phase precipitate to form a garbage homogeneous solution, and adding a mixed enzyme preparation to obtain a first mixture; putting into an enzymolysis tank, putting into a constant-temperature shaking table, shaking to obtain a second mixture, and monitoring the concentration of fermentable sugar in the second mixture to adjust the enzyme amount; according to the invention, the reaction time can be effectively shortened and the required temperature can be reduced; the enzyme hydrolysis inhibition phenomenon caused by too high product concentration is reduced, the enzyme utilization rate is high, the cost is saved, and meanwhile, the reducing sugar is more rapidly released from the biomass.