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Method for promoting food waste to be converted into reducing sugar

A technology for catering waste and fermented sugar, applied in chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem of lack of targeted compound enzyme configuration, high cost of enzymatic treatment, low sugar content, etc. problem, to achieve the effect of reuse, lowering temperature, and lowering the cost of enzymes

Active Publication Date: 2022-04-12
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aiming at the characteristics of high solid content and rich starch and cellulose substances in my country's catering waste, the invention uses the mixed carbohydrase produced by Aspergillus niger and supplements additional protease, and strengthens the insolubility in catering waste by adding enzymes in batches. The hydrolysis of macromolecular sugars greatly increases the concentration of fermentable sugars in catering waste. At the same time, the real-time concentration of fermentable sugars is monitored by the control system to control the dosage of enzyme solution. It provides a feasible method for the configuration of sexual complex enzymes and the high cost of enzymatic treatment

Method used

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  • Method for promoting food waste to be converted into reducing sugar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) if figure 1 As shown, the catering waste is crushed by a cooking machine to a particle size of less than 0.5mm, the oil is extracted by cooking to an oil content of 1.8%, and tap water is added to adjust its TS to 260g / L.

[0041] (2) Mix 1 mol of choline chloride with 6 mol of glycerol without adding water, heat the resulting mixture at 58°C and stir at 200 rpm for 2 hours until a uniform and transparent liquid is formed, and the prepared composition is choline chloride / glycerin deep eutectic solvent.

[0042] (3) Cool the prepared deep eutectic solvent to room temperature, uniformly mix the thick slurry of food waste obtained in step 1 with the deep eutectic solvent at a volume ratio of 1:3, and then perform ultrasonic treatment on it, and the frequency of ultrasonic pretreatment is 20kHz, the amplitude is 50%, the power is 38w, the pulse period is 25 seconds on / 10 seconds off, and the processing time is 22 minutes. After it was cooled to room temperature, it wa...

Embodiment 2

[0048] (1) if figure 1 As shown, the catering waste is crushed by a cooking machine to a particle size of less than 0.5mm, the oil is extracted by cooking to an oil content of 4%, and tap water is added to adjust its TS to 300g / L.

[0049] (2) Add 1mol choline chloride to 12mol glycerin and mix it without water, heat the resulting mixture at 70°C and stir at 120rpm for 1h until a uniform and transparent liquid is formed, the prepared composition is choline chloride / glycerin deep eutectic solvent.

[0050] (3) Cool the prepared deep eutectic solvent to room temperature, uniformly mix the thick slurry of food waste obtained in step 1 with the deep eutectic solvent at a volume ratio of 1:5, and then perform ultrasonic treatment on it, and the frequency of ultrasonic pretreatment is 30kHz, the amplitude is 30%, the power is 30w, the pulse period is 20 seconds on / 10 seconds off, and the processing time is 15 minutes. After it was cooled to room temperature, it was centrifuged, an...

Embodiment 3

[0056] (1) if figure 1 As shown, the catering waste is crushed by a cooking machine to a particle size of less than 0.8mm, the oil is extracted by cooking to an oil content of 2.5%, and tap water is added to adjust its TS to 200g / L.

[0057] (2) Mix 1 mol of choline chloride with 3 mol of glycerol without adding water, heat the resulting mixture at 50°C and stir at 250rpm for 3 hours until a uniform and transparent liquid is formed, the prepared composition is choline chloride / glycerin deep eutectic solvent.

[0058] (3) Cool the prepared deep eutectic solvent to room temperature, uniformly mix the thick slurry of food waste obtained in step 1 with the deep eutectic solvent at a volume ratio of 1:1, and then perform ultrasonic treatment on it, and the frequency of ultrasonic pretreatment is 15kHz, the amplitude is 60%, the power is 50w, the pulse cycle is 30 seconds on / 10 seconds off, and the processing time is 30 minutes. After it was cooled to room temperature, it was cent...

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Abstract

The invention provides a method for promoting conversion of food waste into reducing sugar, which comprises the following specific steps: crushing food waste, extracting oil and pulping to obtain high-concentration food waste thick slurry; the preparation method comprises the following steps: directly mixing choline chloride and glycerol without adding water to prepare a deep eutectic solvent; uniformly mixing the thick food waste slurry with the deep eutectic solvent, and performing ultrasonic centrifugation to collect solid-phase precipitates of the food waste; the method comprises the following steps: inoculating aspergillus into a container containing an enzyme-producing culture medium, culturing, extracting crude enzyme liquid, and adding protease to obtain a mixed enzyme preparation; adding water into the solid-phase precipitate to form a garbage homogeneous solution, and adding a mixed enzyme preparation to obtain a first mixture; putting into an enzymolysis tank, putting into a constant-temperature shaking table, shaking to obtain a second mixture, and monitoring the concentration of fermentable sugar in the second mixture to adjust the enzyme amount; according to the invention, the reaction time can be effectively shortened and the required temperature can be reduced; the enzyme hydrolysis inhibition phenomenon caused by too high product concentration is reduced, the enzyme utilization rate is high, the cost is saved, and meanwhile, the reducing sugar is more rapidly released from the biomass.

Description

technical field [0001] The invention belongs to the field of environmental engineering solid waste treatment, and in particular relates to a method for promoting the transformation of dining waste into reducing sugar. Background technique [0002] With the rapid development of my country's catering industry and the promotion and popularization of waste classification policies, my country's wet waste production has increased year by year. Among them, food waste is one of the main components of wet waste. In 2019, the national food waste production reached 120 million tons. Catering waste is an organic raw material with strong biotransformation potential, containing a large amount of sugar, protein and oil nutrients, which can be used to produce bioenergy such as biogas, organic fertilizer, volatile fatty acids (VFAs) and biomass fuel ethanol. Therefore, the recycling of catering waste has a great market prospect. [0003] However, due to the large size of food waste particle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09B3/35B09B3/60B09B3/70B09B5/00C12P19/02C12P19/12C12R1/685B09B101/70
CPCY02E50/10Y02E50/30
Inventor 郑雄胡婉莹陈银广吴瑒陈玥汐
Owner TONGJI UNIV