Abalone peptide and production method thereof
A production method and abalone technology, applied in the field of abalone peptide and its production, can solve the problems of low enzymatic hydrolysis efficiency, complex operation, short service life, etc., achieve the effects of low free amino acid content, simplify process steps, and improve water resolubility
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Embodiment 1
[0042] (1) Raw material pretreatment: shell the abalone, take the abalone meat, clean it, add water 1 times the weight of the raw material, mash the tissue into a homogenate, then heat it at 100°C for 10 minutes, then cool it down to 50-55°C To be enzymatic solution.
[0043] (2) Enzymolysis: Add 0.1% (w / w) high-efficiency compound enzyme preparation of raw material weight to the enzymolysis solution, mix it evenly, and enzymolyze it under natural conditions without adjusting the pH value and controlling the temperature 1h, the crude abalone peptide solution was obtained. The high-efficiency compound enzyme preparation is composed of 20wt% compound protease, 25wt% alkaline protease, 20wt% acid protease, 25wt% flavor protease, 7wt% animal proteolytic enzyme and 3wt% enzyme activator, wherein, the enzyme activates The agent consists of reducing agent dithiothreitol and divalent metal ion Mg 2+ , Zn 2+ , Ca 2+ Composite composition, the molar ratio of the reducing agent and t...
Embodiment 2
[0049] (1) Raw material pretreatment: shell the abalone, take the abalone meat, clean it, add water three times the weight of the raw material, mash the tissue into a homogenate, then heat at 90°C for 20 minutes, then cool down to 50-55°C to form To be enzymatic solution.
[0050] (2) Enzymolysis: Add 0.1% (w / w) high-efficiency compound enzyme preparation of raw material weight to the enzymolysis solution, mix it evenly, and enzymolyze it under natural conditions without adjusting the pH value and controlling the temperature 1h, the crude abalone peptide solution was obtained. The high-efficiency compound enzyme preparation is composed of 22.5wt% compound protease, 22.5wt% alkaline protease, 22.5wt% acidic protease, 22.5wt% flavor protease, 7.5wt% animal proteolytic enzyme and 2.5wt% enzyme activator, Wherein, the enzyme activator is composed of reducing agent dithiothreitol, β-mercaptoethanol and divalent metal ion Mg 2+ , Zn 2+ , Ca 2+ Composition, the molar ratio of des...
Embodiment 3
[0056] (1) Raw material pretreatment: shell the abalone, take the abalone meat, clean it, add water 4 times the weight of the raw material, mash the tissue into a homogenate, then heat at 80°C for 30 minutes, then cool down to 50-55°C to form To be enzymatic solution.
[0057] (2) Enzymolysis: Add 0.1% (w / w) high-efficiency compound enzyme preparation of raw material weight to the enzymolysis solution, mix it evenly, and enzymolyze it under natural conditions without adjusting the pH value and controlling the temperature 1h, the crude abalone peptide solution was obtained. The high-efficiency compound enzyme preparation is composed of 25wt% compound protease, 20wt% alkaline protease, 25wt% acid protease, 20wt% flavor protease, 8wt% animal proteolytic enzyme and 2wt% enzyme activator, wherein, the enzyme activates The agent consists of reducing agent β-mercaptoethanol and divalent metal ion Mg 2+ , Zn 2+ , Ca 2+ Composition, the molar ratio of described reducing agent and d...
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