Oyster peptide as well as production method and application thereof

A production method and technology for oyster peptide, which are applied to oyster peptide and its production method and application field, can solve the problems of low enzymatic hydrolysis efficiency, acid and alkali resistance, complicated operation, etc., and achieve low free amino acid content and difficult to absorb moisture and agglomerate. , the effect of simplifying the process steps

Inactive Publication Date: 2019-06-18
JIMEI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the problems and shortcomings of the above two invention patent applications are: the enzymatic hydrolysis efficiency of the enzyme used is low, the enzymatic hydrolysis time is long, the amount of enzyme added is large, the optimum pH value and optimum temperature need to be adjusted for enzymatic hydrolysis, and the operation is complicated , adjusting the optimum pH value of enzymatic hydrolysis will bring a large amount of inorganic ions, and controlling the optimum enzymatic hydrolysis temperature consumes a lot of energy; the membrane core used in membrane filtration is organic material, which is not resistant to acid and alkali, high temperature and high pressure, small flux, Short service life and inconvenient cleaning; the prepared oyster peptide powder has poor water resolubility and is easy to absorb moisture during storage

Method used

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  • Oyster peptide as well as production method and application thereof

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Embodiment 1

[0041] (1) Raw material pre-treatment: shell the oysters, take the oyster meat, wash it, add water 1 times the weight of the raw material, mash the tissue into a homogenate, then heat at 100°C for 10 minutes, then cool down to 50-55°C to form To be enzymatic solution.

[0042] (2) Enzymolysis: Add 0.1% (w / w) high-efficiency compound enzyme preparation of raw material weight to the enzymolysis solution, mix it evenly, and enzymolyze it under natural conditions without adjusting the pH value and controlling the temperature 1h, the crude oyster peptide solution was obtained. The high-efficiency compound enzyme preparation is composed of 20wt% compound protease, 25wt% alkaline protease, 20wt% bromelain, 25wt% flavor protease, 7wt% animal proteolytic enzyme and 3wt% enzyme activator, wherein, the enzyme activates The agent consists of reducing agent dithiothreitol and divalent metal ion Mg 2+ , Zn 2+ , Ca 2+ Composite composition, the molar ratio of the reducing agent and the d...

Embodiment 2

[0048] (1) Raw material pre-treatment: shell the oysters, take the oyster meat, clean it, add water three times the weight of the raw material, mash the tissue into a homogenate, then heat at 90°C for 20 minutes, then cool down to 50-55°C to form To be enzymatic solution.

[0049] (2) Enzymolysis: Add 0.1% (w / w) high-efficiency compound enzyme preparation of raw material weight to the enzymolysis solution, mix it evenly, and enzymolyze it under natural conditions without adjusting the pH value and controlling the temperature 1h, the crude oyster peptide solution was obtained. The high-efficiency compound enzyme preparation is composed of 22.5wt% compound protease, 22.5wt% alkaline protease, 22.5wt% bromelain, 22.5wt% flavor protease, 7.5wt% animal proteolytic enzyme and 2.5wt% enzyme activator, Wherein, the enzyme activator is composed of reducing agent dithiothreitol, β-mercaptoethanol and divalent metal ion Mg 2+ , Zn 2+ , Ca 2+ Composition, the molar ratio of described ...

Embodiment 3

[0055] (1) Raw material pre-treatment: Shell the oysters, take the oyster meat, wash it, add water 4 times the weight of the raw material, mash the tissue into a homogenate, then heat at 80°C for 30 minutes, then cool down to 50-55°C to form To be enzymatic solution.

[0056] (2) Enzymolysis: Add 0.1% (w / w) high-efficiency compound enzyme preparation of raw material weight to the enzymolysis solution, mix it evenly, and enzymolyze it under natural conditions without adjusting the pH value and controlling the temperature 1h, the crude oyster peptide solution was obtained. The high-efficiency compound enzyme preparation is composed of 25wt% compound protease, 20wt% alkaline protease, 25wt% bromelain, 20wt% flavor protease, 8wt% animal proteolytic enzyme and 2wt% enzyme activator, wherein, the enzyme activates The agent consists of reducing agent β-mercaptoethanol and divalent metal ion Mg 2+ , Zn 2+ , Ca 2+ Composition, the molar ratio of described reducing agent and divalen...

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Abstract

The invention belongs to the field of oyster peptide and discloses oyster peptide and a production method thereof. The production method of the oyster peptide comprises the following steps: pretreating oysters, performing enzymolysis with a high-efficiency compound enzyme preparation under the conditions that pH value is not adjusted and the temperature is not controlled, performing filtration with an inorganic nanofiltration ceramic membrane to remove insoluble and moisture-absorbing components, and performing high-temperature and high-pressure treatment, concentrating, drying for powder preparation. The method requires no adjustment of enzymolysis pH value or control of enzymolysis temperature, and has the advantages of short enzymolysis time, small enzyme addition quantity and low and concentrated molecular weight of enzymolysis products; prepared oyster peptide powder has excellent water solubility and is not prone to moisture adsorption and caking.

Description

technical field [0001] The invention belongs to the field of oyster peptides, in particular to an oyster peptide and its production method and application. Background technique [0002] Oyster meat is soft and tender, with outstanding umami taste, fishy smell, and unique taste. It is a food with high protein, low fat, rich in glycogen, taurine and a large number of active elements, and it is also a medicinal material in traditional medicine. With the deepening of modern medical research, it has been found that oysters also have a variety of biological activities, such as improving the body's immunity, anti-tumor, and protecting the liver, which has attracted the attention of researchers. [0003] CN102488074A discloses a method for extracting oyster peptides. The process includes cleaning, high-pressure cooking, enzymolysis, deodorization, filtration, concentration, drying, and finished product packaging. Cook at constant temperature and pressure for 2 hours under certain c...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34A23L2/38A23L33/18A23G3/44
Inventor章骞曹敏杰杨爽翁凌刘光明
OwnerJIMEI UNIV