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Cereals alcohol-soluble protein conduit and preparation method thereof

A prolamin and protein tube technology, applied in catheters, medical science, prostheses, etc., can solve the problem of difficult to meet the flexibility of nerve conduits or blood vessels, poor mechanical properties of chitosan and collagen, and no preparation of corn alcohol solubles Problems such as protein hollow tubes, to achieve good biodegradability, uniform roughness, and easy to achieve

Active Publication Date: 2010-06-30
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, due to the inherent defects of the above-mentioned conventional biomaterials: poor mechanical properties of chitosan and collagen, it is difficult to meet the flexibility required by nerve conduits or blood vessels; inflammatory reactions caused by local acidity after degradation of polylactic acid; conduits do not have electrical conductivity. sex etc.
At the same time, there are also the following defects in the process of preparing the catheter: special molds are required; toxic cross-linking agents are used in the process of forming the tube; drying under special conditions that consume energy is required.
The application of electrospinning technology has broadened the processability of zein, however, there is no report on the preparation of zein hollow tubes and their application in the field of tissue engineering tubes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A polytetrafluoroethylene rod with a diameter of 0.28mm is used as the extraction mold, and the protein solution (100mg / ml~150mg / ml, ethanol content 60%~65%) is extracted 4 to 7 times, and the mold is removed after it is thoroughly dried ( It can be seen that the protein tube is transparent); the cereal-like prolamin microsphere solution (cereal-like prolamin 20mg / ml, ethanol content 40%) is extracted again; after it is completely dry, it is immersed in 1mol / L citric acid aqueous solution, and incubated at room temperature for 1 After ~2 hours of drying, a cereal prolamin tube that can meet the size and mechanical requirements of bridging the optic nerve of rats is obtained. The inner diameter of the protein tube is 0.28mm, the average wall thickness is 27μm, the tube length is ≤1cm, the shape of the tube body is good, and the microspheres on the tube wall are evenly distributed. The cereal gliadin is a kind of gliadin derived from corn.

Embodiment 2

[0035] A polytetrafluoroethylene tube with a diameter of 1.6mm is used as the extraction mould, and the protein solution (150mg / ml~200mg / ml, ethanol content 65%~70%) is extracted 7~10 times, and the mold is removed after it is thoroughly dried ( It can be seen that the protein tubes are transparent and uniform throughout); extract the cereal prolamin microsphere solution again (cereal prolamin 20mg / ml, ethanol content 40%); after it is completely dry, immerse in 1mol / L citric acid aqueous solution, room temperature Incubate for 2-3 hours and then dry to obtain a cereal gliadin tube that can meet the size and mechanical requirements of bridging rat sciatic nerves. The inner diameter of the protein tube is 1.6mm, the average wall thickness is 129μm, the tube length is ≤3cm, the shape of the tube body is good, and the microspheres on the tube wall are dense and uniform. The cereal gliadin is a kind of gliadin derived from corn.

Embodiment 3

[0037] A polytetrafluoroethylene tube with a diameter of 12mm is used as the extraction mold, and the protein solution (250mg / ml~300mg / ml, ethanol content 70%~80%) is extracted for 7~10 times, and the mold is removed after it is thoroughly dried (visible The whole body of the protein tube is transparent); extract the cereal-like prolamin microsphere solution again (cereal-like prolamin 20mg / ml, ethanol content 40%); after it is completely dry, immerse in 1mol / L citric acid aqueous solution, and incubate at room temperature for 3~ After 4 hours of drying, polyaniline was deposited on the tube body for 12 hours, and a conductive cereal gliadin tube was obtained. The inner diameter of the protein tube is 12mm, the average wall thickness is 85μm, the tube length is ≤5cm, the tube body is in good shape, the whole body is green and opaque, and the resistance value is 3.9×10 3 Ω, which can meet the conduction requirements of nerve tissue in vivo. The cereal gliadin is a kind of glia...

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Abstract

The invention relates to a cereals alcohol-soluble protein conduit and a preparation method thereof in the field of biomedical technology; the method comprises the following steps: cereals alcohol-soluble protein and organic solvents are mixed to obtain protein solution; after the protein solution is extracted by a mold, drying treatment is carried out and the protein pipe is demoulded from the mold; secondary extraction is carried out to the protein pipe, so as to form a nanoscale spherical structure surface outside, and then form fixation is carried out; finally, conducting polymer is deposited on the protein conduit surface, so as to prepare the conductive cereals alcohol-soluble protein conduit. In the invention, the cereals alcohol-soluble protein is used as raw materials, and the protein conduit has good biocompatibility and degradation, is suitable for preparing tissue engineering conduits and is helpful for regeneration and functional rehabilitation of damaged tubular shaped soft tissues.

Description

technical field [0001] The invention relates to a material in the technical field of biomedicine and a preparation method thereof, in particular to a cereal prolamin tube with an inner diameter of 0.2-12 mm and a tube length of ≤ 5 cm and a preparation method thereof. Background technique [0002] The emergence of the concept of tissue engineering and its development in the past two decades have given us the hope to completely treat the damage and dysfunction of human tissues and organs. This is a new technology to directly obtain new tissues and new organs through cell culture. The most promising therapeutic method of the century [Liu Q, Tissue Engineering (tissue engineering), In: Biomaterials and Tissue Engineering, Shi DL ed, Beijing, Tsinghua University Publishing Press, 2004, p.195.]. In the field of tissue engineering research, biocompatible and degradable materials are usually used to induce regeneration of surrounding tissues or become temporary scaffolds for implan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L29/12A61L27/48
Inventor 王瑾晔狄伶
Owner SHANGHAI JIAO TONG UNIV
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