Homocysteine diagnosing/measuring reagent (kit) and method for measuring concentration of homocysteine

A homocysteine ​​and cystine transhydrogenase technology, which is applied in the field of medical inspection and measurement, can solve the problems of long test time, large variation of test data, complicated operation, etc.

Inactive Publication Date: 2010-06-30
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its characteristics are similar to those of high-performance liquid chromatography, but it has the disadvantages of complicated operation, long test time and large variation of test data.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0050] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0051] Stabilizer 500mmol / L

[0052] Coenzyme 3mmol / L

[0053] Glutathione Homocystine Transhydrogenase 10000U / L

[0054] Glutathione-coenzyme A-glutathione transhydrogenase 12000U / L

[0055] 6-methylsalicylic acid synthase 12000U / L

[0056] Glutathione disulfide 3mmol / L

[0057] Coenzyme A-Glutathione 3mmol / L

[0058] 6-methyl salicylate 5mmol / L

[0059] Sodium bicarbonate 12mmol / L

[0060] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0061] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, volume ratio of tested homocysteine ​​sampl...

Embodiment 2

[0064] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a dual reagent, including:

[0065] Reagent 1

[0066] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0067] Stabilizer 50mmol / L

[0068] Coenzyme 3mmol / L

[0069] Glutathione disulfide 3mmol / L

[0070] Coenzyme A-Glutathione 3mmol / L

[0071] 6-methyl salicylate 5mmol / L

[0072] Sodium bicarbonate 12mmol / L

[0073] Reagent 2

[0074] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0075] Stabilizer 50mmol / L

[0076] Glutathione Homocystine Transhydrogenase 10000U / L

[0077] Glutathione-coenzyme A-glutathione transhydrogenase 12000U / L

[0078] 6-methylsalicylic acid synthase 12000U / L

[0079] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0080] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0...

Embodiment 3

[0083] The homocysteine ​​diagnosis / measurement reagent of this embodiment is three reagents, including:

[0084] Reagent 1

[0085] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0086] Stabilizer 50mmol / L

[0087] Coenzyme 3mmol / L

[0088] Glutathione disulfide 3mmol / L

[0089] Coenzyme A-Glutathione 3mmol / L

[0090] 6-methyl salicylate 5mmol / L

[0091] Sodium bicarbonate 12mmol / L

[0092] Reagent 2

[0093] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0094] Stabilizer 500mmol / L

[0095] Glutathione-coenzyme A-glutathione transhydrogenase 12000U / L

[0096] 6-methylsalicylic acid synthase 12000U / L

[0097] Reagent 3

[0098] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0099] Stabilizer 500mmol / L

[0100] Glutathione Homocystine Transhydrogenase 10000U / L

[0101] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used d...

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PUM

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Abstract

The invention relates to a homocysteine diagnosing/measuring reagent (kit), which utilizes the technology of an enzymic colorimetric method and an enzyme coupled method. And the invention also relates to a method for measuring a concentration of homocysteine and compositions and components of the reagent and belongs to the technical field of the medical testing and measurement. The main components of the reagent (kit) of the invention comprise buffer solution, coenzyme, glutathione disulfide, coenzyme A-glutathione, 6-gaultherolin, sodium bicarbonate (carbon dioxide), glutathione homocysteine transhydrogenase, glutathione-coenzyme A-glutathione transhydrogenase, 6-methl salicylate synzyme and a stabilizing agent. The concentration of the homocysteine is measured by mixing a sample and the reagent in a certain volume ratio to make the sample and the reagent carry out a series of enzymatic reactions, arranging the reactant in an ultraviolet/visible light analyser and detecting the increasing degree of the absorbance at the 340nm position of the dominant wave.

Description

technical field [0001] The invention relates to a homocysteine ​​diagnosis / measurement reagent (box), and at the same time, the invention also relates to a method for measuring the homocysteine ​​concentration, which belongs to the technical field of medical examination and measurement. Background technique [0002] There are many methods for measuring homocysteine ​​in plasma, including high performance liquid chromatography (HPLC), amino acid analyzer assay, ion chromatography, enzyme-linked immunoassay (EIA), capillary gas chromatography-mass spectrometry, fluorescence Polarization immunoassay (FPIA), electrochemical method and enzymatic method, etc. [0003] 1. High-performance liquid chromatography (HPLC): It is a classic reference method, but it has the disadvantages of complicated operation, long test time, and large variation of test data. In clinical application, it is gradually adopted by enzyme-linked immunoassay, Fluorescence polarization analysis and enzymatic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/17G01N33/48C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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