Homocysteine diagnosing/measuring reagent (kit) and method for measuring concentration of homocysteine
A homocysteine and cystine transhydrogenase technology, which is applied in the field of medical inspection and measurement, can solve the problems of long test time, large variation of test data, complicated operation, etc.
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Embodiment 1
[0049] The homocysteine diagnosis / measurement reagent of this embodiment is a single reagent, including:
[0050] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0051] Stabilizer 500mmol / L
[0052] Coenzyme 3mmol / L
[0053] Glutathione Homocystine Transhydrogenase 10000U / L
[0054] Glutathione-coenzyme A-glutathione transhydrogenase 12000U / L
[0055] 6-methylsalicylic acid synthase 12000U / L
[0056] Glutathione disulfide 3mmol / L
[0057] Coenzyme A-Glutathione 3mmol / L
[0058] 6-methyl salicylate 5mmol / L
[0059] Sodium bicarbonate 12mmol / L
[0060] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0061] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, volume ratio of tested homocysteine sampl...
Embodiment 2
[0064] The homocysteine diagnosis / measurement reagent of this embodiment is a dual reagent, including:
[0065] Reagent 1
[0066] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0067] Stabilizer 50mmol / L
[0068] Coenzyme 3mmol / L
[0069] Glutathione disulfide 3mmol / L
[0070] Coenzyme A-Glutathione 3mmol / L
[0071] 6-methyl salicylate 5mmol / L
[0072] Sodium bicarbonate 12mmol / L
[0073] Reagent 2
[0074] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0075] Stabilizer 50mmol / L
[0076] Glutathione Homocystine Transhydrogenase 10000U / L
[0077] Glutathione-coenzyme A-glutathione transhydrogenase 12000U / L
[0078] 6-methylsalicylic acid synthase 12000U / L
[0079] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0080] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0...
Embodiment 3
[0083] The homocysteine diagnosis / measurement reagent of this embodiment is three reagents, including:
[0084] Reagent 1
[0085] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0086] Stabilizer 50mmol / L
[0087] Coenzyme 3mmol / L
[0088] Glutathione disulfide 3mmol / L
[0089] Coenzyme A-Glutathione 3mmol / L
[0090] 6-methyl salicylate 5mmol / L
[0091] Sodium bicarbonate 12mmol / L
[0092] Reagent 2
[0093] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0094] Stabilizer 500mmol / L
[0095] Glutathione-coenzyme A-glutathione transhydrogenase 12000U / L
[0096] 6-methylsalicylic acid synthase 12000U / L
[0097] Reagent 3
[0098] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0099] Stabilizer 500mmol / L
[0100] Glutathione Homocystine Transhydrogenase 10000U / L
[0101] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used d...
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