Rana chensinensis functional gene Rd-RNase3 sequence, construction method and amino acid sequence and application thereof

Abstract

The invention discloses a rana chensinensis functional gene Rd-RNase3 sequence which is characterized by being a nucleotide sequence shown in a sequence table SEQ ID No.1. In the invention, a tumor cell agglutination mediated by the rana chensinensis ribonuclease is not relevant to the selective cytotoxicity of tumor cells and the existence of the wild-type p53 tumor restraint substance, cannot produce gene mutation, and has better stability compared with the prior art. Long-term tumor restraint experiment proves that the Rd-RNase3 gene recombination fusion protein of the invention has strong cytotoxicity on the tumor cells under extremely low concentration, but has no cytotoxicity on normal cells, and thereby having the great advantage on preparing antitumor drugs compared with the existing gene drugs.

Description

technical field

[0001] The invention relates to a functional sequence of Rana chinensis and its coded protein sequence, in particular to a functional gene Rd-RNase3 sequence of Rana chinensis and its coded amino acid sequence and its application. Background technique

[0002] There are 100 million newly diagnosed cancer cases in the world every year, and 250 people die of cancer in every 100,000 people. Therefore, defeating cancer is one of the most important challenges of modern medicine. Although surgery can cure resectable cancers, unresectable cancers can only be treated with chemotherapy or radiation. The main problems of conventional drugs for treatment are lack of selectivity, strong toxicity and immunogenicity of foreign proteins, which often cause side effects, such as temporary diarrhea, nausea, hair loss, and susceptibility to infection. Sometimes long-term effects on the heart, lungs, kidneys, and bone marrow.

[0003] Apoptosis caused by most DNA-degrading dru...

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