Evaluation method of biodegradability of organic matter

An evaluation method, a technology of organic matter, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of complex measurement process, influence on evaluation conclusion, complicated preparation work, etc., and achieve the effect of convenient operation, avoiding result deviation, and simple method

Inactive Publication Date: 2010-08-11
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] ② The measurement period is long and the measurement process is relatively complicated: BOD 5 The measurement generally takes 5 days, the measurement cycle is too long, and the preparatory work is relatively compli...

Method used

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  • Evaluation method of biodegradability of organic matter
  • Evaluation method of biodegradability of organic matter
  • Evaluation method of biodegradability of organic matter

Examples

Experimental program
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Embodiment 1

[0024] Example 1: Biodegradability evaluation of a single organic substance and evaluation results and BOD 5 / COD index comparison

[0025] The embodiment is specifically: select acetone, sodium valerate, aniline, phenol, dichloromethane and 1-chlorobenzene, etc. six common substances in industrial applications as the substance to be tested, and the standard substances are glucose (strong), isobutyl acetate (general) and benzene (poor), the concentration is 150mg / L. The activated sludge taken from the aeration tank of the sewage treatment plant was air-exposed for 12 hours, and diluted with sterile water to 10 7 CFU / mL, while washing and sterilizing the 96-well microplate. There are 8 sample wells for each substance to be tested, 4 of which are blank holes with deionized water instead of the substance to be tested, which is used to subtract the color (absorbance) of the activated sludge solution itself. The loading method of the standard substance is the same as that of the...

Embodiment 2

[0030] Example 2: Biodegradability evaluation and evaluation results and BOD of different concentrations of organic matter 5 / COD index comparison

[0031]The embodiment is specifically as follows: select acetone, sodium valerate and 1-chlorobenzene as three common substances in industrial applications as the substances to be tested, and the standard substances are glucose, isobutyl acetate and benzene. After the activated sludge was air-exposed for 12 hours, it was diluted with sterile water to 10 7 CFU / mL, while washing and sterilizing the 96-well microplate. There are 16 sample holes for each substance to be tested, 4 of which use deionized water instead of the substance to be tested as blank holes to deduct the color (absorbance) of the activated sludge solution itself; the remaining holes are used as concentration gradient holes, followed by 10mg / L, 50mg / L, 100mg / L, 250mg / L, 500mg / L and 1000mg / L, each concentration as a parallel sample, a total of 12 wells. The loading...

Embodiment 3

[0035] Example 3: Biodegradability evaluation of mixed organic compounds and evaluation results and BOD 5 / COD index comparison

[0036] Because in practical application, the target pollutant is usually a mixture, so the evaluation method of the present invention is used to evaluate the biodegradability of the mixed organic compound, and compare it with BOD 5 / COD index comparison. The embodiment is specifically: using vacuum ultraviolet to treat α-pinene waste gas under the conditions of different humidity (2-3%, 35-45% and 75-85%) and different reaction media (high-purity nitrogen and standard air) For photolysis, absorb the photolysis product with phosphate buffer solution (pH7.2). This absorption solution is used as the substance to be tested, and the standard substances are still glucose, isobutyl acetate and benzene. After the activated sludge was air-exposed for 12 hours, it was diluted with sterile water to 10 7 CFU / mL, while washing and sterilizing the 96-well micr...

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Abstract

The invention provides an evaluation method of biodegradability of an organic matter and relates to an evaluation method and a technology of an organic matter environment. In order to solve the problems of measurement error and over long measurement period caused by dilution during BOD5 measurement, the invention uses the growth indicator TTC spectrophotometry to measure the effect of an organic compound on the microbial activity, thereby determining the biodegradability of the organic matter; the method comprises the following steps: using the statistical average method to calculate the corresponding Rsi value; if the Rsi value of the organic matter under measurement is more than 1, the organic matter can be utilized by the microorganism, and the more Rsi value is, the better the biodegradability of the organic matter is; if Rsi value of the organic matter under measurement is lower than 1, the organic matter can hardly be utilized by the microorganism, and the lower Rsi value is, the poorer the biodegradability of the organic matter is. The evaluation method used by the invention is simple and quick, can be used to measure the biodegradability of a single organic compound, also can be used to measure the biodegradability of a mixture of unknown component, and simultaneously avoids the measurement error caused by the high dilution.

Description

(1) Technical field [0001] The invention relates to an evaluation method for the biodegradability of organic matter, belonging to the field of environmental behavior evaluation of environmental pollutants. (2) Background technology [0002] Biological treatment of organic waste has the advantages of low cost, easy operation, and no secondary pollution, and has attracted the attention of many researchers in recent years. When using biological methods, the biodegradability of target pollutants must be considered. Currently commonly used biodegradability evaluation indicators are BOD 5 / COD, BOD 5 / TOC, BOD 5 / TOC, etc., the most commonly used evaluation index is BOD 5 / COD. Looking at these evaluation indicators, they all involve BOD 5 Determination. [0003] BOD 5 It is the five-day biochemical oxygen demand, which refers to the amount of dissolved oxygen consumed by microorganisms to decompose organic matter in water at a water temperature of 20°C and cultured for 5 ...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 蒋轶锋成卓韦陈建孟
Owner ZHEJIANG UNIV OF TECH
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