Compound separated and extracted from marine penicillium and application thereof

A compound, the technology of Penicillium, applied in the field of marine microorganisms, can solve the problems such as no effective results reported, and achieve the effects of obvious inhibition of quorum sensing, simple and easy-to-control process, and outstanding social benefits

Inactive Publication Date: 2013-01-30
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

By screening marine fungal extracts, marine fungi with quorum sensing inhibitory activity of Pseudomonas aeruginosa are found, and compounds with quorum sensing inhibitory activity of Pseudomonas aeruginosa are isolated from their extracts, which is the technical field researchers are discussing topics, so far no effective results have been reported

Method used

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  • Compound separated and extracted from marine penicillium and application thereof
  • Compound separated and extracted from marine penicillium and application thereof
  • Compound separated and extracted from marine penicillium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Acquisition of marine Penicillium QJ012 CCTCC No.M 209218

[0016] 1. Strain acquisition

[0017] 1. Seawater and sea mud samples for cultivation were collected from Qianhai and Jiaozhou Bay in Qingdao.

[0018] 2. Isolation and cultivation of marine fungi: the following culture medium is used for fungal purification: peel the potatoes, wash them, slice them, weigh 200g, put them in 1000mL of old sea water, boil them with a slow fire for 10-20min, filter them with four layers of gauze, add water to the filtrate to make up to 1000mL , add glucose 20g, agar 20g, 121℃, 1.05kg / cm 2 Sterilize for 20 minutes, add 30 μg / mL of penicillin and streptomycin before use; weigh about 1 g of sea mud in a sterile state, add 10 mL of sterile seawater, shake and mix, and then dilute 10 times and 100 times respectively to make a total of For 3 concentration gradient samples, take 0.2mL each and spread it on the solid medium, and spread multiple plates for each gradient; take ...

Embodiment 2

[0028] Embodiment 2: Fermentation marine penicillium prepares compound

[0029] 1. Fermentation culture uses the following medium: sorbitol 20g, maltose 20g, glutamine 10g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.3g, tryptophan 0.5g, yeast extract 3g, dilute to 1000mL with aged sea water, adjust pH to 6.5, 121°C, 1.05kg / cm 2 Sterilize for 20 minutes.

[0030] 2. Fermentation process: After activating the plate of Penicillium marinum QJ012, pick an appropriate amount of mycelia and inoculate it into a Erlenmeyer flask containing 200 mL of the above-mentioned liquid medium. The temperature is 28° C., the rotation speed is 140 rpm, and the culture is shaken for 10 days.

[0031] 3. Fermentation product extraction: After the fermentation, ultrasonically break the fermented bacteria and supernatant mixture, add an equal volume of ethyl acetate, stir overnight at room temperature, centrifuge at 6000rpm for 10min to take the supernatant, then add an equal volume of ethyl acetate and re...

Embodiment 3

[0039] Embodiment 3: activity test of formula (I) compound

[0040] One. The effect of different concentrations of formula (I) compounds on the growth of wild-type Pseudomonas aeruginosa PAO1

[0041] 1. Pick wild-type Pseudomonas aeruginosa PAO1 in fresh LB medium, and culture it at 37°C and 140rpm until logarithmic phase;

[0042] 2. Dilute the bacteria cultured to the logarithmic phase with fresh LB to OD 600 0.05, divided into 5 test tubes, 5mL in each tube, added the compound of formula (I) to make the final concentration respectively 0mg / L, 6.25mg / L, 12.5mg / L, 25mg / L, 50mg / L, 37℃ , 140rpm shaker culture;

[0043] 3. Measure the OD of each tube every 1-2 hours 600 Until the late logarithmic period; take the culture time (h) as the abscissa, and take the light absorbance OD at 600nm 600 For the ordinate, draw the growth curve of wild type Pseudomonas aeruginosa PAO1 under the effect of formula (I) compound, from growth curve ( figure 2 ) It can be seen that the compo...

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PUM

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Abstract

The invention belongs to the technical field of preparing compounds, and relates to a compound separated and extracted from marine penicillium and application thereof. The compound is used for preventing and treating in-vitra and in-vivo pseudomonas aeruginosa infection of people or animals, and the chemical formula of the compound which is obtained by fermenting the marine penicillium and has the effect of inhibiting quorum sensing of pseudomonas aeruginosa is 4,6-dimethyl-7-[(1R,2E,4aS,7R,8R,8aR)-1,2.4a,5,6,7,8,8a-octahydro-7-hydroxyl-2-(3-hydroxyl-2-oxygen-propylene)-3,6,8-trimethyl-1-naphthyl]-,(2E,4E,6E)-heptantriene acid, and the molecular formula of the compound is C25H34O5; the compound does not inhibit the growth of the wild type pseudomonas aeruginosa; the quorum-sensing inhibition effect of the compound is concentration-dependent, and is gradually strengthened with the increase of the concentration; virulence factors controlled by the quorum sensing of the pseudomonas aeruginosa can be inhibited; and the compound also has the advantages of readily available raw materials, simple and easily controlled preparation process, obvious sensing inhibition effect and prominent social benefits.

Description

Technical field: [0001] The invention belongs to the technical field of marine microorganisms, and relates to a compound isolated from marine Penicillium sp. Prevention and treatment of Pseudomonas infection. Background technique: [0002] With the widespread use of antibacterial drugs, bacterial resistance has become more and more serious, and has become a major problem that endangers human health worldwide. Finding new ways to treat bacterial infections has become the frontier and hot spot of life science research. Quorum Sensing (QS) is a bacterial population behavior regulation system that has received widespread attention recently. Studies have found that the pathogenesis of many human or plant pathogenic bacteria is regulated by the QS system, and many bacterial metabolites are also controlled by this system. Therefore, the purpose of weakening the pathogenicity of animal and plant pathogens can be achieved by interfering with the QS system of pathogenic bacteria. Its...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C59/90C12P7/42A61K31/192A61P31/04C12R1/80
Inventor 于文功宫倩红邹姗姗刘红兵尹守亮
Owner OCEAN UNIV OF CHINA
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